Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5572 and PP_5573 are separated by 703 nucleotides PP_5573 and PP_3102 are separated by 89 nucleotides PP_3102 and PP_3103 overlap by 4 nucleotides
PP_5572: PP_5572 - protein of unknown function, at 3,503,024 to 3,503,305
_5572
PP_5573: PP_5573 - protein of unknown function, at 3,504,009 to 3,504,185
_5573
PP_3102: PP_3102 - conserved protein of unknown function, at 3,504,275 to 3,504,571
_3102
PP_3103: PP_3103 - conserved protein of unknown function, at 3,504,568 to 3,505,641
_3103
Position (kb)
3504
3505 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3503.427 kb on - strand at 3503.454 kb on - strand at 3503.454 kb on - strand at 3503.586 kb on + strand at 3503.608 kb on - strand at 3503.701 kb on + strand at 3503.762 kb on - strand at 3503.803 kb on - strand at 3503.980 kb on + strand at 3504.010 kb on + strand at 3504.090 kb on + strand, within PP_5573 at 3504.207 kb on + strand at 3504.208 kb on - strand at 3504.208 kb on - strand at 3504.214 kb on + strand at 3504.215 kb on - strand at 3504.216 kb on + strand at 3504.217 kb on - strand at 3504.217 kb on - strand at 3504.320 kb on + strand, within PP_3102 at 3504.321 kb on - strand, within PP_3102 at 3504.325 kb on - strand, within PP_3102 at 3504.422 kb on - strand, within PP_3102 at 3504.456 kb on + strand, within PP_3102 at 3504.470 kb on + strand, within PP_3102 at 3504.473 kb on + strand, within PP_3102 at 3504.484 kb on - strand, within PP_3102 at 3504.493 kb on - strand, within PP_3102 at 3504.542 kb on + strand at 3504.542 kb on + strand at 3504.542 kb on + strand at 3504.542 kb on + strand at 3504.543 kb on - strand at 3504.543 kb on - strand at 3504.543 kb on - strand at 3504.571 kb on + strand at 3504.571 kb on + strand at 3504.571 kb on + strand at 3504.587 kb on - strand at 3504.647 kb on + strand at 3504.647 kb on + strand at 3504.648 kb on - strand at 3504.694 kb on - strand, within PP_3103 at 3504.694 kb on - strand, within PP_3103 at 3504.729 kb on - strand, within PP_3103 at 3504.828 kb on - strand, within PP_3103 at 3504.932 kb on + strand, within PP_3103 at 3504.933 kb on - strand, within PP_3103 at 3504.933 kb on - strand, within PP_3103 at 3504.941 kb on + strand, within PP_3103 at 3504.941 kb on + strand, within PP_3103 at 3504.954 kb on + strand, within PP_3103 at 3504.955 kb on - strand, within PP_3103 at 3504.956 kb on + strand, within PP_3103 at 3504.957 kb on - strand, within PP_3103 at 3504.957 kb on - strand, within PP_3103 at 3504.979 kb on + strand, within PP_3103 at 3505.003 kb on + strand, within PP_3103 at 3505.003 kb on + strand, within PP_3103 at 3505.003 kb on + strand, within PP_3103 at 3505.004 kb on - strand, within PP_3103 at 3505.004 kb on - strand, within PP_3103 at 3505.332 kb on + strand, within PP_3103 at 3505.333 kb on - strand, within PP_3103 at 3505.337 kb on + strand, within PP_3103 at 3505.337 kb on + strand, within PP_3103 at 3505.337 kb on + strand, within PP_3103 at 3505.338 kb on - strand, within PP_3103 at 3505.338 kb on - strand, within PP_3103 at 3505.338 kb on - strand, within PP_3103 at 3505.357 kb on + strand, within PP_3103 at 3505.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,503,427 - -0.6 3,503,454 - +0.8 3,503,454 - -1.4 3,503,586 + +1.0 3,503,608 - -1.3 3,503,701 + +0.5 3,503,762 - -0.3 3,503,803 - -1.7 3,503,980 + +0.2 3,504,010 + +0.3 3,504,090 + PP_5573 0.46 +0.2 3,504,207 + +1.0 3,504,208 - -0.2 3,504,208 - -0.2 3,504,214 + -0.9 3,504,215 - -0.4 3,504,216 + -0.7 3,504,217 - -1.6 3,504,217 - -0.1 3,504,320 + PP_3102 0.15 +3.4 3,504,321 - PP_3102 0.15 +1.5 3,504,325 - PP_3102 0.17 +1.8 3,504,422 - PP_3102 0.49 -0.2 3,504,456 + PP_3102 0.61 +0.7 3,504,470 + PP_3102 0.66 +0.1 3,504,473 + PP_3102 0.67 +1.1 3,504,484 - PP_3102 0.70 +5.1 3,504,493 - PP_3102 0.73 -0.2 3,504,542 + -0.3 3,504,542 + +1.5 3,504,542 + +0.3 3,504,542 + -1.2 3,504,543 - +0.7 3,504,543 - -1.2 3,504,543 - -0.0 3,504,571 + +0.2 3,504,571 + +1.7 3,504,571 + -1.8 3,504,587 - -1.0 3,504,647 + -1.3 3,504,647 + -0.0 3,504,648 - -0.9 3,504,694 - PP_3103 0.12 -0.2 3,504,694 - PP_3103 0.12 +0.4 3,504,729 - PP_3103 0.15 -0.6 3,504,828 - PP_3103 0.24 +0.5 3,504,932 + PP_3103 0.34 -0.9 3,504,933 - PP_3103 0.34 -1.2 3,504,933 - PP_3103 0.34 -0.9 3,504,941 + PP_3103 0.35 -0.1 3,504,941 + PP_3103 0.35 -0.3 3,504,954 + PP_3103 0.36 +0.8 3,504,955 - PP_3103 0.36 +1.0 3,504,956 + PP_3103 0.36 -1.3 3,504,957 - PP_3103 0.36 -1.6 3,504,957 - PP_3103 0.36 -3.8 3,504,979 + PP_3103 0.38 -1.0 3,505,003 + PP_3103 0.41 -1.8 3,505,003 + PP_3103 0.41 +0.9 3,505,003 + PP_3103 0.41 -1.5 3,505,004 - PP_3103 0.41 +1.0 3,505,004 - PP_3103 0.41 +1.7 3,505,332 + PP_3103 0.71 +0.4 3,505,333 - PP_3103 0.71 +0.1 3,505,337 + PP_3103 0.72 -1.2 3,505,337 + PP_3103 0.72 +0.2 3,505,337 + PP_3103 0.72 +0.6 3,505,338 - PP_3103 0.72 +0.5 3,505,338 - PP_3103 0.72 +0.7 3,505,338 - PP_3103 0.72 +0.1 3,505,357 + PP_3103 0.73 -0.6 3,505,542 - -0.3
Or see this region's nucleotide sequence