Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaP and yoaK are separated by 32 nucleotides yoaK and yoaJ are separated by 2 nucleotides yoaJ and yeaQ are separated by 58 nucleotides yeaQ and yoaG are separated by 147 nucleotides yoaG and yeaR are separated by 3 nucleotides
b1794: yeaP - orf, hypothetical protein (VIMSS), at 1,875,739 to 1,876,764
yeaP
b4676: yoaK - expressed protein (RefSeq), at 1,876,797 to 1,876,895
yoaK
b4675: yoaJ - expressed protein (RefSeq), at 1,876,898 to 1,876,972
yoaJ
b1795: yeaQ - conserved inner membrane protein (NCBI), at 1,877,031 to 1,877,279
yeaQ
b1796: yoaG - hypothetical protein (NCBI), at 1,877,427 to 1,877,609
yoaG
b1797: yeaR - hypothetical protein (NCBI), at 1,877,613 to 1,877,972
yeaR
Position (kb)
1876
1877 Strain fitness (log2 ratio)
-1
0
1
2 at 1875.848 kb on - strand, within yeaP at 1876.009 kb on + strand, within yeaP at 1876.093 kb on + strand, within yeaP at 1876.241 kb on - strand, within yeaP at 1876.257 kb on + strand, within yeaP at 1876.257 kb on + strand, within yeaP at 1876.257 kb on + strand, within yeaP at 1876.257 kb on + strand, within yeaP at 1876.257 kb on + strand, within yeaP at 1876.347 kb on + strand, within yeaP at 1876.361 kb on - strand, within yeaP at 1876.377 kb on + strand, within yeaP at 1876.377 kb on + strand, within yeaP at 1876.421 kb on - strand, within yeaP at 1876.421 kb on - strand, within yeaP at 1876.452 kb on + strand, within yeaP at 1876.480 kb on + strand, within yeaP at 1876.506 kb on + strand, within yeaP at 1876.616 kb on + strand, within yeaP at 1876.639 kb on + strand, within yeaP at 1876.639 kb on + strand, within yeaP at 1876.668 kb on + strand at 1876.678 kb on - strand at 1876.707 kb on + strand at 1876.753 kb on + strand at 1876.753 kb on + strand at 1876.753 kb on + strand at 1876.770 kb on + strand at 1876.879 kb on - strand, within yoaK at 1876.879 kb on - strand, within yoaK at 1876.889 kb on - strand at 1876.889 kb on - strand at 1876.945 kb on + strand, within yoaJ at 1876.945 kb on + strand, within yoaJ at 1876.955 kb on + strand, within yoaJ at 1876.963 kb on - strand, within yoaJ at 1876.963 kb on - strand, within yoaJ at 1877.005 kb on + strand at 1877.014 kb on - strand at 1877.035 kb on + strand at 1877.135 kb on - strand, within yeaQ at 1877.137 kb on + strand, within yeaQ at 1877.145 kb on - strand, within yeaQ at 1877.145 kb on - strand, within yeaQ at 1877.174 kb on - strand, within yeaQ at 1877.175 kb on - strand, within yeaQ at 1877.260 kb on + strand at 1877.433 kb on + strand at 1877.476 kb on + strand, within yoaG at 1877.476 kb on + strand, within yoaG at 1877.484 kb on - strand, within yoaG at 1877.513 kb on - strand, within yoaG at 1877.571 kb on - strand, within yoaG at 1877.602 kb on - strand at 1877.658 kb on - strand, within yeaR at 1877.663 kb on + strand, within yeaR at 1877.663 kb on + strand, within yeaR at 1877.714 kb on + strand, within yeaR at 1877.715 kb on - strand, within yeaR at 1877.764 kb on + strand, within yeaR at 1877.765 kb on + strand, within yeaR at 1877.765 kb on + strand, within yeaR at 1877.791 kb on + strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.814 kb on - strand, within yeaR at 1877.859 kb on - strand, within yeaR
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 1,875,848 - yeaP b1794 0.11 -1.0 1,876,009 + yeaP b1794 0.26 +0.4 1,876,093 + yeaP b1794 0.35 +0.7 1,876,241 - yeaP b1794 0.49 -1.5 1,876,257 + yeaP b1794 0.50 -0.4 1,876,257 + yeaP b1794 0.50 -0.7 1,876,257 + yeaP b1794 0.50 +0.4 1,876,257 + yeaP b1794 0.50 -0.6 1,876,257 + yeaP b1794 0.50 +0.4 1,876,347 + yeaP b1794 0.59 -0.1 1,876,361 - yeaP b1794 0.61 -0.1 1,876,377 + yeaP b1794 0.62 -0.1 1,876,377 + yeaP b1794 0.62 -0.7 1,876,421 - yeaP b1794 0.66 -0.4 1,876,421 - yeaP b1794 0.66 +0.6 1,876,452 + yeaP b1794 0.69 -0.1 1,876,480 + yeaP b1794 0.72 -1.3 1,876,506 + yeaP b1794 0.75 -0.2 1,876,616 + yeaP b1794 0.85 -0.2 1,876,639 + yeaP b1794 0.88 +0.4 1,876,639 + yeaP b1794 0.88 +0.9 1,876,668 + -0.4 1,876,678 - +1.8 1,876,707 + -0.2 1,876,753 + -0.2 1,876,753 + -0.3 1,876,753 + +0.5 1,876,770 + +0.5 1,876,879 - yoaK b4676 0.83 +0.2 1,876,879 - yoaK b4676 0.83 +1.8 1,876,889 - -0.2 1,876,889 - -0.5 1,876,945 + yoaJ b4675 0.63 -0.1 1,876,945 + yoaJ b4675 0.63 -0.9 1,876,955 + yoaJ b4675 0.76 -0.2 1,876,963 - yoaJ b4675 0.87 +0.2 1,876,963 - yoaJ b4675 0.87 +0.8 1,877,005 + +0.8 1,877,014 - +0.6 1,877,035 + -0.5 1,877,135 - yeaQ b1795 0.42 -0.5 1,877,137 + yeaQ b1795 0.43 +0.4 1,877,145 - yeaQ b1795 0.46 +1.2 1,877,145 - yeaQ b1795 0.46 -0.0 1,877,174 - yeaQ b1795 0.57 -0.1 1,877,175 - yeaQ b1795 0.58 +0.0 1,877,260 + +0.3 1,877,433 + +0.5 1,877,476 + yoaG b1796 0.27 -1.5 1,877,476 + yoaG b1796 0.27 +0.1 1,877,484 - yoaG b1796 0.31 +0.2 1,877,513 - yoaG b1796 0.47 -0.2 1,877,571 - yoaG b1796 0.79 +0.4 1,877,602 - -0.4 1,877,658 - yeaR b1797 0.12 +0.3 1,877,663 + yeaR b1797 0.14 -0.7 1,877,663 + yeaR b1797 0.14 +0.0 1,877,714 + yeaR b1797 0.28 -0.6 1,877,715 - yeaR b1797 0.28 -1.1 1,877,764 + yeaR b1797 0.42 -1.4 1,877,765 + yeaR b1797 0.42 +0.6 1,877,765 + yeaR b1797 0.42 +0.1 1,877,791 + yeaR b1797 0.49 -0.4 1,877,804 - yeaR b1797 0.53 -1.0 1,877,804 - yeaR b1797 0.53 +0.5 1,877,804 - yeaR b1797 0.53 +0.1 1,877,814 - yeaR b1797 0.56 +0.8 1,877,859 - yeaR b1797 0.68 -1.1
Or see this region's nucleotide sequence