Strain Fitness in Escherichia coli BW25113 around b0077

Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
84,635	+	leuO	b0076	0.28	-0.1
84,635	+	leuO	b0076	0.28	-0.3
84,644	-	leuO	b0076	0.29	+2.1
84,648	+	leuO	b0076	0.30	-0.5
84,648	+	leuO	b0076	0.30	-1.5
84,648	+	leuO	b0076	0.30	+2.1
84,648	+	leuO	b0076	0.30	+0.1
84,658	-	leuO	b0076	0.31	+1.1
84,658	-	leuO	b0076	0.31	+1.1
84,671	+	leuO	b0076	0.32	-1.3
84,696	+	leuO	b0076	0.35	+0.3
84,696	+	leuO	b0076	0.35	+0.5
84,728	+	leuO	b0076	0.38	+0.4
84,728	+	leuO	b0076	0.38	+0.7
84,728	+	leuO	b0076	0.38	+0.5
84,728	+	leuO	b0076	0.38	-0.6
84,731	+	leuO	b0076	0.38	-0.9
84,736	-	leuO	b0076	0.39	+2.1
84,736	-	leuO	b0076	0.39	+2.5
84,769	+	leuO	b0076	0.42	-2.0
84,786	+	leuO	b0076	0.44	-0.1
84,786	+	leuO	b0076	0.44	-0.6
84,799	+	leuO	b0076	0.46	-0.1
84,799	+	leuO	b0076	0.46	+0.5
84,805	-	leuO	b0076	0.46	+2.1
84,805	-	leuO	b0076	0.46	+1.1
84,825	+	leuO	b0076	0.48	+1.7
84,825	-	leuO	b0076	0.48	-0.9
84,827	+	leuO	b0076	0.49	-0.5
84,827	+	leuO	b0076	0.49	-1.1
84,851	-	leuO	b0076	0.51	+0.5
84,859	+	leuO	b0076	0.52	+0.4
84,867	+	leuO	b0076	0.53	+0.8
84,875	-	leuO	b0076	0.54	+2.1
84,900	-	leuO	b0076	0.56	+1.5
84,918	+	leuO	b0076	0.58	-0.3
84,918	+	leuO	b0076	0.58	+1.3
84,922	-	leuO	b0076	0.59	-1.5
84,947	-	leuO	b0076	0.61	-1.3
84,947	-	leuO	b0076	0.61	+0.5
84,975	-	leuO	b0076	0.64	-1.2
84,975	-	leuO	b0076	0.64	-0.2
84,978	-	leuO	b0076	0.65	+0.9
84,978	-	leuO	b0076	0.65	+0.2
84,990	-	leuO	b0076	0.66	-0.1
85,019	-	leuO	b0076	0.69	+1.1
85,028	+	leuO	b0076	0.70	+0.4
85,028	+	leuO	b0076	0.70	-0.1
85,037	-	leuO	b0076	0.71	-0.2
85,040	+	leuO	b0076	0.71	+1.5
85,045	+	leuO	b0076	0.72	+0.2
85,045	+	leuO	b0076	0.72	+0.4
85,048	-	leuO	b0076	0.72	-0.1
85,049	+	leuO	b0076	0.72	-0.5
85,050	+	leuO	b0076	0.72	+0.1
85,051	-	leuO	b0076	0.72	+0.1
85,051	-	leuO	b0076	0.72	+2.5
85,055	+	leuO	b0076	0.73	-1.8
85,055	+	leuO	b0076	0.73	+0.3
85,055	+	leuO	b0076	0.73	-0.9
85,058	-	leuO	b0076	0.73	-0.9
85,063	-	leuO	b0076	0.74	+0.9
85,063	-	leuO	b0076	0.74	+1.1
85,063	-	leuO	b0076	0.74	-0.4
85,064	-	leuO	b0076	0.74	-0.7
85,065	-	leuO	b0076	0.74	-0.5
85,066	+	leuO	b0076	0.74	-0.1
85,068	+	leuO	b0076	0.74	-1.8
85,069	-	leuO	b0076	0.74	+0.6
85,070	+	leuO	b0076	0.74	-0.4
85,071	+	leuO	b0076	0.74	-0.3
85,071	+	leuO	b0076	0.74	-1.0
85,071	+	leuO	b0076	0.74	-1.4
85,074	-	leuO	b0076	0.75	-0.9
85,076	+	leuO	b0076	0.75	-0.8
85,076	-	leuO	b0076	0.75	+0.2
85,076	-	leuO	b0076	0.75	-0.5
85,077	+	leuO	b0076	0.75	-0.7
85,079	+	leuO	b0076	0.75	-1.2
85,079	+	leuO	b0076	0.75	+0.2
85,079	-	leuO	b0076	0.75	-0.5
85,086	+	leuO	b0076	0.76	+0.3
85,086	+	leuO	b0076	0.76	+0.1
85,087	-	leuO	b0076	0.76	-0.2
85,087	-	leuO	b0076	0.76	+0.8
85,087	-	leuO	b0076	0.76	+0.7
85,098	+	leuO	b0076	0.77	-0.2
85,098	+	leuO	b0076	0.77	-0.3
85,098	+	leuO	b0076	0.77	+0.4
85,099	-	leuO	b0076	0.77	+0.4
85,099	-	leuO	b0076	0.77	+0.5
85,099	-	leuO	b0076	0.77	+0.8
85,102	-	leuO	b0076	0.78	+1.1
85,104	+	leuO	b0076	0.78	-0.9
85,104	+	leuO	b0076	0.78	+0.3
85,104	+	leuO	b0076	0.78	-0.7
85,104	+	leuO	b0076	0.78	-0.1
85,104	+	leuO	b0076	0.78	-0.5
85,104	+	leuO	b0076	0.78	+0.9
85,104	+	leuO	b0076	0.78	+0.8
85,105	+	leuO	b0076	0.78	+1.3
85,105	+	leuO	b0076	0.78	+0.3
85,108	-	leuO	b0076	0.78	-0.5
85,108	-	leuO	b0076	0.78	-1.5
85,108	-	leuO	b0076	0.78	-0.3
85,108	-	leuO	b0076	0.78	+2.1
85,110	+	leuO	b0076	0.79	-0.5
85,112	-	leuO	b0076	0.79	+1.5
85,112	-	leuO	b0076	0.79	+1.1
85,112	-	leuO	b0076	0.79	-1.2
85,137	+	leuO	b0076	0.81	-0.1
85,137	+	leuO	b0076	0.81	+0.0
85,137	+	leuO	b0076	0.81	+0.4
85,137	+	leuO	b0076	0.81	-0.6
85,145	-	leuO	b0076	0.82	+2.1
85,145	-	leuO	b0076	0.82	+1.1
85,147	+	leuO	b0076	0.82	-0.7
85,157	+	leuO	b0076	0.83	-0.0
85,157	+	leuO	b0076	0.83	-1.9
85,157	+	leuO	b0076	0.83	+0.1
85,157	+	leuO	b0076	0.83	-1.0
85,216	-	leuO	b0076	0.90	+2.1
85,224	+				-1.0
85,224	+				-1.1
85,224	-				+1.1
85,227	+				-1.1
85,227	+				+0.1
85,234	+				-0.7
85,234	+				+0.0
85,234	+				-0.4
85,234	+				-0.1
85,240	+				-0.1
85,250	+				-0.5
85,250	+				-0.6
85,259	+				-0.8
85,266	-				-0.5
85,273	+				-0.8
85,277	+				-0.3
85,292	+				-1.3
85,327	+				-0.7
85,327	+				-1.8
85,327	+				-0.8
85,332	+				-1.6
85,332	+				+0.2
85,334	+				-0.4
85,388	+				-1.4
85,388	+				-1.6
85,548	-				-0.9
85,548	-				-0.7
85,582	-				-0.1
85,582	-				-3.0
85,584	+				+1.8
85,592	-				-0.7
85,620	+				+1.7
85,623	-				-2.5
85,626	+				-0.3
85,626	+				-0.1
85,626	+				-1.3
85,626	+				+0.4
85,626	-				-1.3
85,627	+				-0.9
85,627	+				+0.5
85,634	-				-0.2
85,634	-				-1.5
85,634	-				-1.4
85,634	-				-2.6
85,635	-				-2.2
85,635	-				-1.1
85,635	-				-1.3
85,635	-				-1.9
85,636	-				-0.6
85,638	-				-1.9
85,643	-				-1.8
85,643	-				-2.1
85,646	-				-1.8
85,646	-				-2.7
85,649	-				-2.8
85,649	-				-3.0
85,725	-				+0.4
85,725	-				-0.9
85,738	+				-2.2
85,738	+				-1.2
85,745	-				-2.7
85,745	-				-0.2
85,757	-				-0.9
85,765	+				-2.8
85,793	-				-0.8
85,794	-				-1.3
85,802	-				-1.6
85,802	-				-1.0
85,802	-				-0.9
85,802	-				-1.6
85,815	-	ilvI	b0077	0.11	-0.6
85,815	-	ilvI	b0077	0.11	-1.3
85,830	+	ilvI	b0077	0.12	-1.6
85,856	+	ilvI	b0077	0.13	-1.4
85,856	+	ilvI	b0077	0.13	-1.3
85,864	-	ilvI	b0077	0.14	-2.2
85,931	+	ilvI	b0077	0.17	-1.9
85,937	+	ilvI	b0077	0.18	-1.8
86,112	+	ilvI	b0077	0.28	-1.3
86,348	+	ilvI	b0077	0.42	-1.9
86,348	+	ilvI	b0077	0.42	-1.2
86,357	+	ilvI	b0077	0.42	-2.1
86,357	+	ilvI	b0077	0.42	-0.5
86,366	-	ilvI	b0077	0.43	-2.5
86,367	+	ilvI	b0077	0.43	-1.6
86,438	+	ilvI	b0077	0.47	-0.9
86,447	-	ilvI	b0077	0.47	-0.5
86,447	-	ilvI	b0077	0.47	-1.5
86,508	+	ilvI	b0077	0.51	-2.2
86,517	+	ilvI	b0077	0.51	-1.5
86,535	-	ilvI	b0077	0.52	-1.2
86,535	-	ilvI	b0077	0.52	-2.3
86,548	-	ilvI	b0077	0.53	-2.0
86,593	-	ilvI	b0077	0.56	-1.6
86,602	+	ilvI	b0077	0.56	-1.7
86,602	+	ilvI	b0077	0.56	-1.1
86,797	+	ilvI	b0077	0.68	-1.9
86,841	+	ilvI	b0077	0.70	-2.3
86,894	-	ilvI	b0077	0.73	-2.9
86,899	+	ilvI	b0077	0.74	-0.6
87,001	-	ilvI	b0077	0.79	-2.4
87,026	+	ilvI	b0077	0.81	-1.7
87,034	-	ilvI	b0077	0.81	-1.7
87,062	-	ilvI	b0077	0.83	-2.9
87,070	+	ilvI	b0077	0.83	-2.3
87,070	+	ilvI	b0077	0.83	-0.6
87,092	-	ilvI	b0077	0.85	-2.6
87,098	-	ilvI	b0077	0.85	-2.9
87,098	-	ilvI	b0077	0.85	-2.7
87,130	+	ilvI	b0077	0.87	-2.4
87,165	-	ilvI	b0077	0.89	-1.1
87,166	-	ilvI	b0077	0.89	-3.0
87,250	+				-0.7
87,285	+				-1.5
87,443	-	ilvH	b0078	0.17	+1.5
87,480	+	ilvH	b0078	0.25	-2.0
87,497	+	ilvH	b0078	0.28	-2.1
87,571	+	ilvH	b0078	0.43	-1.8
87,571	+	ilvH	b0078	0.43	-2.4
87,588	-	ilvH	b0078	0.47	-1.1
87,617	-	ilvH	b0078	0.53	-0.7
87,617	-	ilvH	b0078	0.53	-3.5
87,640	+	ilvH	b0078	0.58	-1.1
87,665	-	ilvH	b0078	0.63	+0.6
87,698	-	ilvH	b0078	0.69	+1.1
87,705	+	ilvH	b0078	0.71	-1.3
87,710	+	ilvH	b0078	0.72	-2.0
87,710	+	ilvH	b0078	0.72	+0.0
87,718	-	ilvH	b0078	0.73	-2.1
87,718	-	ilvH	b0078	0.73	-1.1
87,718	-	ilvH	b0078	0.73	-2.7
87,718	-	ilvH	b0078	0.73	-3.0
87,740	-	ilvH	b0078	0.78	-2.2
87,744	-	ilvH	b0078	0.79	-2.1
87,785	+	ilvH	b0078	0.87	-2.2
87,802	+				-2.4
87,815	-				-1.5
87,918	-				-0.4
87,951	-				-0.1
87,955	+				-0.2
88,009	+				-0.3
88,074	+				-0.8
88,085	+				+1.4
88,111	-				-0.2
88,111	-				-0.5
88,111	-				-0.5
88,111	-				-0.8
88,113	-				-0.2
88,187	+	fruR	b0080	0.16	+0.1
88,187	+	fruR	b0080	0.16	-0.0
88,259	-	fruR	b0080	0.23	-0.4
88,265	+	fruR	b0080	0.24	+0.3