Strain Fitness in Escherichia coli BW25113 around b3368

Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
3,494,888	-				+0.4
3,494,888	-				+0.3
3,494,939	+				+0.0
3,494,939	+				-0.1
3,494,947	-				+0.5
3,494,947	-				-0.9
3,495,048	+				+0.1
3,495,056	-				-0.0
3,495,062	-				+0.2
3,495,071	+				-0.5
3,495,079	-				-0.5
3,495,113	+	nirC	b3367	0.11	-1.7
3,495,113	+	nirC	b3367	0.11	-1.4
3,495,123	+	nirC	b3367	0.12	+0.9
3,495,124	-	nirC	b3367	0.12	-0.6
3,495,124	-	nirC	b3367	0.12	-0.2
3,495,129	+	nirC	b3367	0.13	+1.0
3,495,129	+	nirC	b3367	0.13	+1.1
3,495,131	+	nirC	b3367	0.13	+0.3
3,495,148	-	nirC	b3367	0.15	-0.8
3,495,148	-	nirC	b3367	0.15	+0.9
3,495,148	-	nirC	b3367	0.15	-0.3
3,495,148	-	nirC	b3367	0.15	+0.3
3,495,182	+	nirC	b3367	0.19	-0.0
3,495,195	-	nirC	b3367	0.21	+0.4
3,495,197	+	nirC	b3367	0.21	+0.4
3,495,197	+	nirC	b3367	0.21	-0.7
3,495,251	+	nirC	b3367	0.28	-1.1
3,495,251	+	nirC	b3367	0.28	+0.7
3,495,251	+	nirC	b3367	0.28	+0.7
3,495,259	-	nirC	b3367	0.29	-0.7
3,495,271	-	nirC	b3367	0.30	-1.4
3,495,308	+	nirC	b3367	0.35	+0.4
3,495,308	+	nirC	b3367	0.35	+0.3
3,495,311	-	nirC	b3367	0.35	-0.1
3,495,311	-	nirC	b3367	0.35	+0.1
3,495,353	-	nirC	b3367	0.41	+0.2
3,495,358	+	nirC	b3367	0.41	+0.2
3,495,388	+	nirC	b3367	0.45	+0.4
3,495,388	+	nirC	b3367	0.45	-0.2
3,495,388	+	nirC	b3367	0.45	+0.4
3,495,388	+	nirC	b3367	0.45	-0.5
3,495,388	+	nirC	b3367	0.45	-0.3
3,495,396	-	nirC	b3367	0.46	-0.0
3,495,491	+	nirC	b3367	0.58	-0.3
3,495,535	+	nirC	b3367	0.63	-0.3
3,495,579	-	nirC	b3367	0.69	-0.5
3,495,609	+	nirC	b3367	0.72	+0.5
3,495,623	-	nirC	b3367	0.74	-0.1
3,495,623	-	nirC	b3367	0.74	-0.2
3,495,626	+	nirC	b3367	0.74	-1.6
3,495,626	+	nirC	b3367	0.74	-0.4
3,495,645	+	nirC	b3367	0.77	-0.1
3,495,645	+	nirC	b3367	0.77	+0.9
3,495,653	+	nirC	b3367	0.78	-0.4
3,495,654	-	nirC	b3367	0.78	-0.0
3,495,678	-	nirC	b3367	0.81	+0.2
3,495,678	-	nirC	b3367	0.81	-1.2
3,495,702	+	nirC	b3367	0.84	-0.5
3,495,705	-	nirC	b3367	0.84	-1.2
3,495,710	-	nirC	b3367	0.85	-0.7
3,495,715	-	nirC	b3367	0.86	+0.6
3,495,717	+	nirC	b3367	0.86	-0.5
3,495,744	-	nirC	b3367	0.89	+0.1
3,495,832	-				-0.5
3,495,893	+				-3.0
3,495,932	-				-0.6
3,495,936	+				-3.1
3,495,983	+				-2.7
3,495,993	+	cysG	b3368	0.10	-1.7
3,496,019	+	cysG	b3368	0.12	-2.0
3,496,019	+	cysG	b3368	0.12	-0.5
3,496,019	+	cysG	b3368	0.12	-1.3
3,496,026	-	cysG	b3368	0.13	-2.9
3,496,065	-	cysG	b3368	0.16	-1.2
3,496,070	-	cysG	b3368	0.16	-3.3
3,496,100	+	cysG	b3368	0.18	-3.2
3,496,100	+	cysG	b3368	0.18	-1.0
3,496,119	-	cysG	b3368	0.20	-2.6
3,496,119	-	cysG	b3368	0.20	-2.2
3,496,237	-	cysG	b3368	0.28	-1.6
3,496,300	-	cysG	b3368	0.33	-0.6
3,496,300	-	cysG	b3368	0.33	-2.1
3,496,345	+	cysG	b3368	0.36	-2.7
3,496,351	+	cysG	b3368	0.36	-2.5
3,496,371	-	cysG	b3368	0.38	-1.8
3,496,417	-	cysG	b3368	0.41	-1.6
3,496,422	-	cysG	b3368	0.42	-1.9
3,496,456	-	cysG	b3368	0.44	+0.7
3,496,502	-	cysG	b3368	0.47	-2.8
3,496,504	+	cysG	b3368	0.48	-3.2
3,496,504	+	cysG	b3368	0.48	-1.0
3,496,514	+	cysG	b3368	0.48	-1.3
3,496,514	+	cysG	b3368	0.48	-1.3
3,496,514	+	cysG	b3368	0.48	-0.7
3,496,519	-	cysG	b3368	0.49	-1.8
3,496,529	+	cysG	b3368	0.49	-2.5
3,496,529	+	cysG	b3368	0.49	-0.8
3,496,539	+	cysG	b3368	0.50	-1.3
3,496,539	+	cysG	b3368	0.50	-3.0
3,496,582	-	cysG	b3368	0.53	-2.8
3,496,582	-	cysG	b3368	0.53	-1.2
3,496,582	-	cysG	b3368	0.53	-3.0
3,496,585	+	cysG	b3368	0.53	-1.4
3,496,585	+	cysG	b3368	0.53	-0.8
3,496,585	+	cysG	b3368	0.53	-2.1
3,496,585	-	cysG	b3368	0.53	-1.4
3,496,617	-	cysG	b3368	0.56	-2.4
3,496,622	-	cysG	b3368	0.56	-2.9
3,496,634	+	cysG	b3368	0.57	-1.2
3,496,666	+	cysG	b3368	0.59	-2.9
3,496,666	+	cysG	b3368	0.59	-1.0
3,496,675	+	cysG	b3368	0.60	-3.5
3,496,675	+	cysG	b3368	0.60	-0.7
3,496,679	-	cysG	b3368	0.60	-0.9
3,496,679	-	cysG	b3368	0.60	-1.5
3,496,682	-	cysG	b3368	0.61	-3.6
3,496,697	+	cysG	b3368	0.62	-3.1
3,496,697	+	cysG	b3368	0.62	-2.6
3,496,702	+	cysG	b3368	0.62	-1.6
3,496,702	+	cysG	b3368	0.62	-2.6
3,496,708	-	cysG	b3368	0.62	-0.1
3,496,786	+	cysG	b3368	0.68	-2.3
3,496,794	-	cysG	b3368	0.69	-0.1
3,496,797	+	cysG	b3368	0.69	-2.7
3,496,800	+	cysG	b3368	0.69	-2.3
3,496,800	+	cysG	b3368	0.69	-2.3
3,496,800	+	cysG	b3368	0.69	-1.5
3,496,823	+	cysG	b3368	0.71	-1.7
3,496,823	+	cysG	b3368	0.71	-2.6
3,496,825	+	cysG	b3368	0.71	-1.6
3,496,835	+	cysG	b3368	0.72	-0.2
3,496,841	-	cysG	b3368	0.72	-0.4
3,496,846	+	cysG	b3368	0.72	-2.4
3,496,861	+	cysG	b3368	0.74	-3.1
3,496,861	+	cysG	b3368	0.74	+0.2
3,496,861	+				-0.8
3,496,877	+	cysG	b3368	0.75	-2.5
3,496,877	+	cysG	b3368	0.75	-1.6
3,496,892	+	cysG	b3368	0.76	-0.6
3,496,892	+	cysG	b3368	0.76	-3.1
3,496,904	+	cysG	b3368	0.77	-1.2
3,496,904	+	cysG	b3368	0.77	-2.5
3,496,942	+	cysG	b3368	0.79	-1.4
3,496,942	+	cysG	b3368	0.79	-1.4
3,496,947	+	cysG	b3368	0.80	-2.2
3,496,960	-	cysG	b3368	0.81	-0.4
3,496,981	+	cysG	b3368	0.82	-3.6
3,496,981	+	cysG	b3368	0.82	-1.8
3,496,986	+	cysG	b3368	0.83	-1.4
3,496,986	+	cysG	b3368	0.83	-2.8
3,496,986	+	cysG	b3368	0.83	-2.6
3,496,986	+	cysG	b3368	0.83	-2.2
3,496,988	+	cysG	b3368	0.83	-1.8
3,496,989	+	cysG	b3368	0.83	-1.4
3,496,989	+	cysG	b3368	0.83	-1.3
3,496,989	+	cysG	b3368	0.83	-2.9
3,496,991	+	cysG	b3368	0.83	-3.5
3,496,994	-	cysG	b3368	0.83	+1.2
3,496,994	-	cysG	b3368	0.83	+0.2
3,496,994	-	cysG	b3368	0.83	+1.2
3,496,996	+	cysG	b3368	0.83	-1.1
3,496,996	+	cysG	b3368	0.83	-1.1
3,497,012	+	cysG	b3368	0.85	-1.7
3,497,017	+	cysG	b3368	0.85	-1.8
3,497,054	+	cysG	b3368	0.88	-0.5
3,497,089	+				-0.4
3,497,097	+				-1.2
3,497,097	+				-2.6
3,497,113	+				-1.0
3,497,114	+				-2.2
3,497,114	+				-2.5
3,497,114	+				-2.6
3,497,114	+				-3.3
3,497,114	+				-1.3
3,497,114	+				-0.7
3,497,116	+				-2.0
3,497,117	+				-2.0
3,497,118	+				-2.3
3,497,124	+				-2.8
3,497,124	+				-2.0
3,497,124	+				-1.0
3,497,130	-				+2.2
3,497,136	+				-3.0
3,497,142	+				-1.5
3,497,142	+				-1.6
3,497,143	+				-1.5
3,497,143	+				-2.9
3,497,146	+				-2.4
3,497,159	+				-1.3
3,497,165	+				-1.2
3,497,172	+				-1.0
3,497,173	+				-2.4
3,497,173	+				-1.8
3,497,180	+				-1.3
3,497,180	+				-2.1
3,497,180	+				-2.3
3,497,180	+				-3.7
3,497,190	+				-1.7
3,497,192	+				-1.0
3,497,192	+				-0.4
3,497,192	+				-0.6
3,497,192	+				+1.2
3,497,192	+				-1.8
3,497,192	+				-0.9
3,497,192	+				-1.9
3,497,193	+				+0.0
3,497,205	+				-0.2
3,497,205	+				+1.2
3,497,205	+				-0.6
3,497,213	+				+0.4
3,497,454	-				-0.6
3,497,454	-				-1.2
3,497,494	+	yhfL	b3369	0.14	+1.2
3,497,497	+	yhfL	b3369	0.16	+1.2
3,497,501	+	yhfL	b3369	0.18	-1.4
3,497,501	+	yhfL	b3369	0.18	-0.1
3,497,504	-	yhfL	b3369	0.20	+0.6
3,497,504	-	yhfL	b3369	0.20	+0.5
3,497,506	+	yhfL	b3369	0.21	+1.5
3,497,506	-	yhfL	b3369	0.21	+0.2
3,497,508	+	yhfL	b3369	0.23	-0.6
3,497,508	+	yhfL	b3369	0.23	+2.2
3,497,509	-	yhfL	b3369	0.23	+0.4
3,497,509	-	yhfL	b3369	0.23	-0.1
3,497,509	-	yhfL	b3369	0.23	-1.3
3,497,511	+	yhfL	b3369	0.24	-1.6
3,497,514	-	yhfL	b3369	0.26	+0.3
3,497,514	-	yhfL	b3369	0.26	+0.2
3,497,514	-	yhfL	b3369	0.26	+0.9
3,497,514	-	yhfL	b3369	0.26	+0.2
3,497,514	-	yhfL	b3369	0.26	+0.5
3,497,514	-	yhfL	b3369	0.26	+0.7
3,497,514	-	yhfL	b3369	0.26	+0.6
3,497,514	-	yhfL	b3369	0.26	+0.1
3,497,514	-	yhfL	b3369	0.26	+0.0
3,497,516	+	yhfL	b3369	0.27	+1.2
3,497,516	+	yhfL	b3369	0.27	-0.1
3,497,518	-	yhfL	b3369	0.29	-0.2
3,497,518	-	yhfL	b3369	0.29	+0.6
3,497,518	-	yhfL	b3369	0.29	-0.4
3,497,518	-	yhfL	b3369	0.29	-1.2
3,497,519	-	yhfL	b3369	0.29	+0.1
3,497,519	-	yhfL	b3369	0.29	-0.2
3,497,519	-	yhfL	b3369	0.29	+0.2
3,497,522	+	yhfL	b3369	0.31	+1.4
3,497,522	+	yhfL	b3369	0.31	+1.1
3,497,522	+	yhfL	b3369	0.31	-0.5
3,497,524	-	yhfL	b3369	0.32	-0.5
3,497,524	-	yhfL	b3369	0.32	+0.5
3,497,524	-	yhfL	b3369	0.32	-0.1
3,497,524	-	yhfL	b3369	0.32	+0.2
3,497,524	-	yhfL	b3369	0.32	+0.9
3,497,527	-	yhfL	b3369	0.34	-0.4
3,497,527	-	yhfL	b3369	0.34	+1.2
3,497,531	-	yhfL	b3369	0.36	+1.3
3,497,531	-	yhfL	b3369	0.36	-2.4
3,497,534	-	yhfL	b3369	0.38	-0.3
3,497,534	-	yhfL	b3369	0.38	-0.3
3,497,536	-	yhfL	b3369	0.39	-0.9
3,497,538	-	yhfL	b3369	0.40	-2.4
3,497,547	-	yhfL	b3369	0.46	-0.1
3,497,556	+	yhfL	b3369	0.51	+0.7
3,497,559	-	yhfL	b3369	0.53	-0.6
3,497,562	+	yhfL	b3369	0.55	+1.4
3,497,563	+	yhfL	b3369	0.55	+0.1
3,497,563	+	yhfL	b3369	0.55	-1.0
3,497,563	+	yhfL	b3369	0.55	-0.9
3,497,567	+	yhfL	b3369	0.58	-1.0
3,497,569	-	yhfL	b3369	0.59	+0.3
3,497,571	-	yhfL	b3369	0.60	+0.3
3,497,571	-	yhfL	b3369	0.60	-1.8
3,497,571	-	yhfL	b3369	0.60	+0.0
3,497,572	-	yhfL	b3369	0.61	+1.0
3,497,575	+	yhfL	b3369	0.62	+0.0
3,497,575	+	yhfL	b3369	0.62	-1.8
3,497,580	-	yhfL	b3369	0.65	-0.7
3,497,580	-	yhfL	b3369	0.65	-1.0
3,497,580	-	yhfL	b3369	0.65	+0.1
3,497,583	-	yhfL	b3369	0.67	-0.0
3,497,619	+	yhfL	b3369	0.89	-0.2
3,497,621	+				-0.7
3,497,624	+				-0.1
3,497,627	+				-0.5
3,497,627	-				-0.2
3,497,629	+				-1.1
3,497,629	+				-0.4
3,497,629	-				+0.3
3,497,640	-				-0.9
3,497,711	-				+0.8
3,497,777	-				+0.7
3,497,784	-				-0.7
3,497,784	-				+0.2
3,497,788	+				-0.7
3,497,788	+				-0.7
3,497,801	-				-0.1
3,497,896	+				-0.1
3,497,904	-				-0.6
3,497,907	-				-0.1
3,497,937	-				+0.3
3,497,939	+				-0.4
3,497,939	+				+1.1
3,498,022	-				-1.0
3,498,025	+				-0.6
3,498,025	+				-0.4
3,498,034	-				-0.9
3,498,034	-				+0.8
3,498,048	+				-0.0
3,498,059	+				+1.4
3,498,059	+				-0.2
3,498,059	+				+0.0
3,498,061	-				-0.1
3,498,067	-	yhfM	b3370	0.10	+0.7
3,498,067	-	yhfM	b3370	0.10	-0.7
3,498,077	-	yhfM	b3370	0.11	+0.1
3,498,077	-	yhfM	b3370	0.11	+0.4
3,498,085	+	yhfM	b3370	0.11	-2.1
3,498,089	+	yhfM	b3370	0.12	+0.5
3,498,111	-	yhfM	b3370	0.13	-1.1
3,498,119	+	yhfM	b3370	0.14	+0.2
3,498,129	+	yhfM	b3370	0.15	+0.3
3,498,129	+	yhfM	b3370	0.15	-0.4
3,498,137	-	yhfM	b3370	0.15	-1.0
3,498,137	-	yhfM	b3370	0.15	-1.6
3,498,157	+	yhfM	b3370	0.17	+0.7
3,498,196	+	yhfM	b3370	0.20	+0.2
3,498,196	+	yhfM	b3370	0.20	-0.6
3,498,203	+	yhfM	b3370	0.20	+0.9
3,498,214	+	yhfM	b3370	0.21	-0.5