Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt yhdP and cafA are separated by 67 nucleotides cafA and yhdE overlap by 11 nucleotides yhdE and mreD are separated by 8 nucleotides 
        b4472: yhdP - conserved membrane protein, predicted transporter (NCBI), at 3,390,480 to 3,394,280 
        yhdP 
         
        
        b3247: cafA - bundles of cytoplasmic filaments (VIMSS), at 3,394,348 to 3,395,817 
        cafA 
         
        
        b3248: yhdE - Maf-like protein (NCBI), at 3,395,807 to 3,396,400 
        yhdE 
         
        
        b3249: mreD - cell wall structural complex MreBCD transmembrane component MreD (NCBI), at 3,396,409 to 3,396,897 
        mreD 
         Position (kb) 3394 
3395 
3396 Strain fitness (log2 ratio) -2 
-1 
0 
1 at 3393.446 kb on + strand, within yhdP at 3393.446 kb on + strand, within yhdP at 3393.496 kb on - strand, within yhdP at 3393.496 kb on - strand, within yhdP at 3393.555 kb on - strand, within yhdP at 3393.561 kb on - strand, within yhdP at 3393.574 kb on + strand, within yhdP at 3393.574 kb on + strand, within yhdP at 3393.605 kb on + strand, within yhdP at 3393.646 kb on + strand, within yhdP at 3393.677 kb on - strand, within yhdP at 3393.730 kb on - strand, within yhdP at 3393.749 kb on + strand, within yhdP at 3393.749 kb on + strand, within yhdP at 3393.768 kb on + strand, within yhdP at 3393.772 kb on - strand, within yhdP at 3393.773 kb on + strand, within yhdP at 3393.773 kb on + strand, within yhdP at 3393.791 kb on - strand, within yhdP at 3393.884 kb on - strand, within yhdP at 3393.895 kb on - strand, within yhdP at 3393.895 kb on - strand, within yhdP at 3393.897 kb on + strand, within yhdP at 3393.905 kb on - strand at 3393.906 kb on + strand at 3393.989 kb on - strand at 3394.001 kb on - strand at 3394.001 kb on - strand at 3394.062 kb on + strand at 3394.062 kb on + strand at 3394.171 kb on - strand at 3394.188 kb on + strand at 3394.207 kb on - strand at 3394.220 kb on - strand at 3394.235 kb on - strand at 3394.279 kb on + strand at 3394.343 kb on - strand at 3394.353 kb on + strand at 3394.354 kb on - strand at 3394.354 kb on - strand at 3394.354 kb on - strand at 3394.467 kb on + strand at 3394.475 kb on - strand at 3394.486 kb on + strand at 3394.486 kb on + strand at 3394.486 kb on + strand at 3394.617 kb on + strand, within cafA at 3394.626 kb on - strand, within cafA at 3394.651 kb on + strand, within cafA at 3394.651 kb on + strand, within cafA at 3394.722 kb on + strand, within cafA at 3394.818 kb on + strand, within cafA at 3394.898 kb on + strand, within cafA at 3394.922 kb on + strand, within cafA at 3395.036 kb on + strand, within cafA at 3395.041 kb on - strand, within cafA at 3395.041 kb on - strand, within cafA at 3395.044 kb on - strand, within cafA at 3395.097 kb on - strand, within cafA at 3395.105 kb on - strand, within cafA at 3395.121 kb on + strand, within cafA at 3395.139 kb on - strand, within cafA at 3395.188 kb on + strand, within cafA at 3395.193 kb on + strand, within cafA at 3395.201 kb on - strand, within cafA at 3395.231 kb on + strand, within cafA at 3395.254 kb on - strand, within cafA at 3395.254 kb on - strand, within cafA at 3395.272 kb on - strand, within cafA at 3395.283 kb on + strand, within cafA at 3395.291 kb on - strand, within cafA at 3395.292 kb on + strand, within cafA at 3395.292 kb on + strand, within cafA at 3395.318 kb on - strand, within cafA at 3395.318 kb on - strand, within cafA at 3395.328 kb on - strand, within cafA at 3395.330 kb on + strand, within cafA at 3395.330 kb on + strand, within cafA at 3395.335 kb on + strand, within cafA at 3395.387 kb on - strand, within cafA at 3395.397 kb on + strand, within cafA at 3395.397 kb on + strand, within cafA at 3395.414 kb on - strand, within cafA at 3395.479 kb on - strand, within cafA at 3395.532 kb on + strand, within cafA at 3395.532 kb on + strand, within cafA at 3395.542 kb on + strand, within cafA at 3395.560 kb on - strand, within cafA at 3395.568 kb on - strand, within cafA at 3395.568 kb on - strand, within cafA at 3395.599 kb on + strand, within cafA at 3395.599 kb on + strand, within cafA at 3395.666 kb on - strand, within cafA at 3395.677 kb on + strand at 3395.689 kb on + strand at 3395.690 kb on + strand at 3395.690 kb on + strand at 3395.787 kb on + strand at 3395.893 kb on - strand, within yhdE at 3395.897 kb on - strand, within yhdE at 3395.897 kb on - strand, within yhdE at 3395.923 kb on + strand, within yhdE at 3395.923 kb on + strand, within yhdE at 3395.941 kb on + strand, within yhdE at 3395.956 kb on - strand, within yhdE at 3395.964 kb on - strand, within yhdE at 3395.966 kb on + strand, within yhdE at 3395.987 kb on - strand, within yhdE at 3395.987 kb on - strand, within yhdE at 3396.058 kb on - strand, within yhdE at 3396.117 kb on - strand, within yhdE at 3396.139 kb on + strand, within yhdE at 3396.166 kb on - strand, within yhdE at 3396.166 kb on - strand, within yhdE at 3396.176 kb on - strand, within yhdE at 3396.186 kb on - strand, within yhdE at 3396.192 kb on + strand, within yhdE at 3396.231 kb on + strand, within yhdE at 3396.231 kb on + strand, within yhdE at 3396.256 kb on + strand, within yhdE at 3396.267 kb on + strand, within yhdE at 3396.269 kb on + strand, within yhdE at 3396.275 kb on - strand, within yhdE at 3396.275 kb on - strand, within yhdE at 3396.287 kb on - strand, within yhdE at 3396.287 kb on - strand, within yhdE at 3396.307 kb on - strand, within yhdE at 3396.317 kb on - strand, within yhdE at 3396.333 kb on - strand, within yhdE at 3396.344 kb on - strand at 3396.344 kb on - strand at 3396.364 kb on - strand at 3396.364 kb on - strand at 3396.417 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 3,393,446 +  yhdP  b4472 0.78  -0.9 3,393,446 +  yhdP  b4472 0.78  -0.4 3,393,496 -  yhdP  b4472 0.79  -0.3 3,393,496 -  yhdP  b4472 0.79  -0.1 3,393,555 -  yhdP  b4472 0.81  -0.3 3,393,561 -  yhdP  b4472 0.81  -0.9 3,393,574 +  yhdP  b4472 0.81  -0.7 3,393,574 +  yhdP  b4472 0.81  +0.4 3,393,605 +  yhdP  b4472 0.82  -0.2 3,393,646 +  yhdP  b4472 0.83  -0.3 3,393,677 -  yhdP  b4472 0.84  -0.4 3,393,730 -  yhdP  b4472 0.86  -1.3 3,393,749 +  yhdP  b4472 0.86  -0.0 3,393,749 +  yhdP  b4472 0.86  -1.0 3,393,768 +  yhdP  b4472 0.87  -0.1 3,393,772 -  yhdP  b4472 0.87  -1.0 3,393,773 +  yhdP  b4472 0.87  -1.5 3,393,773 +  yhdP  b4472 0.87  -0.5 3,393,791 -  yhdP  b4472 0.87  -1.6 3,393,884 -  yhdP  b4472 0.90  -0.5 3,393,895 -  yhdP  b4472 0.90  -1.2 3,393,895 -  yhdP  b4472 0.90  -2.7 3,393,897 +  yhdP  b4472 0.90  -0.7 3,393,905 -  -0.5 3,393,906 +  +0.7 3,393,989 -  -1.4 3,394,001 -  -1.8 3,394,001 -  -2.6 3,394,062 +  -1.3 3,394,062 +  -0.2 3,394,171 -  -0.2 3,394,188 +  +0.0 3,394,207 -  -1.6 3,394,220 -  -1.1 3,394,235 -  +0.5 3,394,279 +  -0.6 3,394,343 -  +0.1 3,394,353 +  -0.3 3,394,354 -  -0.9 3,394,354 -  -0.3 3,394,354 -  -0.6 3,394,467 +  +0.2 3,394,475 -  +0.5 3,394,486 +  -0.6 3,394,486 +  -1.1 3,394,486 +  -0.2 3,394,617 +  cafA  b3247 0.18  -1.2 3,394,626 -  cafA  b3247 0.19  -1.2 3,394,651 +  cafA  b3247 0.21  -2.0 3,394,651 +  cafA  b3247 0.21  -0.7 3,394,722 +  cafA  b3247 0.25  -1.0 3,394,818 +  cafA  b3247 0.32  -2.4 3,394,898 +  cafA  b3247 0.37  -1.3 3,394,922 +  cafA  b3247 0.39  -0.4 3,395,036 +  cafA  b3247 0.47  -0.2 3,395,041 -  cafA  b3247 0.47  -2.1 3,395,041 -  cafA  b3247 0.47  -0.4 3,395,044 -  cafA  b3247 0.47  -1.2 3,395,097 -  cafA  b3247 0.51  -0.4 3,395,105 -  cafA  b3247 0.51  -1.5 3,395,121 +  cafA  b3247 0.53  -0.9 3,395,139 -  cafA  b3247 0.54  -0.6 3,395,188 +  cafA  b3247 0.57  -1.3 3,395,193 +  cafA  b3247 0.57  -1.0 3,395,201 -  cafA  b3247 0.58  -2.0 3,395,231 +  cafA  b3247 0.60  -1.6 3,395,254 -  cafA  b3247 0.62  -0.4 3,395,254 -  cafA  b3247 0.62  +0.3 3,395,272 -  cafA  b3247 0.63  -0.3 3,395,283 +  cafA  b3247 0.64  -0.8 3,395,291 -  cafA  b3247 0.64  -0.8 3,395,292 +  cafA  b3247 0.64  -1.2 3,395,292 +  cafA  b3247 0.64  +0.2 3,395,318 -  cafA  b3247 0.66  -2.6 3,395,318 -  cafA  b3247 0.66  -0.2 3,395,328 -  cafA  b3247 0.67  -1.1 3,395,330 +  cafA  b3247 0.67  -0.4 3,395,330 +  cafA  b3247 0.67  -0.5 3,395,335 +  cafA  b3247 0.67  -2.4 3,395,387 -  cafA  b3247 0.71  +0.2 3,395,397 +  cafA  b3247 0.71  -1.9 3,395,397 +  cafA  b3247 0.71  -1.7 3,395,414 -  cafA  b3247 0.73  -2.4 3,395,479 -  cafA  b3247 0.77  -0.8 3,395,532 +  cafA  b3247 0.81  -1.3 3,395,532 +  cafA  b3247 0.81  -0.4 3,395,542 +  cafA  b3247 0.81  -1.1 3,395,560 -  cafA  b3247 0.82  -0.4 3,395,568 -  cafA  b3247 0.83  -1.5 3,395,568 -  cafA  b3247 0.83  -0.0 3,395,599 +  cafA  b3247 0.85  -0.8 3,395,599 +  cafA  b3247 0.85  -1.7 3,395,666 -  cafA  b3247 0.90  -2.5 3,395,677 +  -0.8 3,395,689 +  -1.8 3,395,690 +  -1.1 3,395,690 +  -1.5 3,395,787 +  -0.9 3,395,893 -  yhdE  b3248 0.14  +0.8 3,395,897 -  yhdE  b3248 0.15  +0.9 3,395,897 -  yhdE  b3248 0.15  +1.1 3,395,923 +  yhdE  b3248 0.20  -1.0 3,395,923 +  yhdE  b3248 0.20  -0.6 3,395,941 +  yhdE  b3248 0.23  -0.8 3,395,956 -  yhdE  b3248 0.25  -0.3 3,395,964 -  yhdE  b3248 0.26  +0.4 3,395,966 +  yhdE  b3248 0.27  +0.4 3,395,987 -  yhdE  b3248 0.30  +0.3 3,395,987 -  yhdE  b3248 0.30  +0.5 3,396,058 -  yhdE  b3248 0.42  +0.3 3,396,117 -  yhdE  b3248 0.52  +0.5 3,396,139 +  yhdE  b3248 0.56  -0.1 3,396,166 -  yhdE  b3248 0.60  +1.0 3,396,166 -  yhdE  b3248 0.60  +0.4 3,396,176 -  yhdE  b3248 0.62  +0.6 3,396,186 -  yhdE  b3248 0.64  +0.2 3,396,192 +  yhdE  b3248 0.65  -0.2 3,396,231 +  yhdE  b3248 0.71  -0.1 3,396,231 +  yhdE  b3248 0.71  +0.7 3,396,256 +  yhdE  b3248 0.76  -0.0 3,396,267 +  yhdE  b3248 0.77  +0.8 3,396,269 +  yhdE  b3248 0.78  -0.1 3,396,275 -  yhdE  b3248 0.79  +1.0 3,396,275 -  yhdE  b3248 0.79  -0.3 3,396,287 -  yhdE  b3248 0.81  -0.2 3,396,287 -  yhdE  b3248 0.81  -0.7 3,396,307 -  yhdE  b3248 0.84  +0.4 3,396,317 -  yhdE  b3248 0.86  -0.4 3,396,333 -  yhdE  b3248 0.89  -1.1 3,396,344 -  -0.3 3,396,344 -  -1.6 3,396,364 -  +0.8 3,396,364 -  +1.1 3,396,417 -  -0.2 
Or see this region's nucleotide sequence