Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speA and yqgB are separated by 8 nucleotides yqgB and yqgC are separated by 135 nucleotides yqgC and metK are separated by 303 nucleotides
b2938: speA - arginine decarboxylase (NCBI), at 3,081,957 to 3,083,933
speA
b2939: yqgB - predicted protein (RefSeq), at 3,083,942 to 3,084,073
yqgB
b2940: yqgC - hypothetical protein (NCBI), at 3,084,209 to 3,084,424
yqgC
b2942: metK - S-adenosylmethionine synthetase (NCBI), at 3,084,728 to 3,085,882
metK
Position (kb)
3083
3084
3085 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3083.018 kb on + strand, within speA at 3083.040 kb on + strand, within speA at 3083.040 kb on + strand, within speA at 3083.045 kb on + strand, within speA at 3083.045 kb on + strand, within speA at 3083.142 kb on + strand, within speA at 3083.142 kb on + strand, within speA at 3083.287 kb on - strand, within speA at 3083.287 kb on - strand, within speA at 3083.305 kb on + strand, within speA at 3083.305 kb on + strand, within speA at 3083.361 kb on + strand, within speA at 3083.361 kb on + strand, within speA at 3083.365 kb on - strand, within speA at 3083.365 kb on - strand, within speA at 3083.408 kb on + strand, within speA at 3083.416 kb on - strand, within speA at 3083.440 kb on - strand, within speA at 3083.440 kb on - strand, within speA at 3083.452 kb on - strand, within speA at 3083.452 kb on - strand, within speA at 3083.462 kb on - strand, within speA at 3083.575 kb on - strand, within speA at 3083.575 kb on - strand, within speA at 3083.618 kb on - strand, within speA at 3083.677 kb on - strand, within speA at 3083.693 kb on + strand, within speA at 3083.693 kb on + strand, within speA at 3083.761 kb on - strand at 3083.772 kb on - strand at 3083.802 kb on - strand at 3083.823 kb on + strand at 3083.834 kb on - strand at 3083.834 kb on - strand at 3083.872 kb on + strand at 3083.874 kb on + strand at 3083.880 kb on - strand at 3083.880 kb on - strand at 3083.911 kb on + strand at 3083.985 kb on + strand, within yqgB at 3084.042 kb on + strand, within yqgB at 3084.150 kb on - strand at 3084.153 kb on + strand at 3084.166 kb on - strand at 3084.181 kb on - strand at 3084.227 kb on + strand at 3084.242 kb on + strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.285 kb on - strand, within yqgC at 3084.345 kb on - strand, within yqgC at 3084.471 kb on + strand at 3084.632 kb on + strand at 3084.632 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 3,083,018 + speA b2938 0.54 -0.7 3,083,040 + speA b2938 0.55 +0.1 3,083,040 + speA b2938 0.55 +0.0 3,083,045 + speA b2938 0.55 +0.7 3,083,045 + speA b2938 0.55 +1.1 3,083,142 + speA b2938 0.60 -0.9 3,083,142 + speA b2938 0.60 +0.4 3,083,287 - speA b2938 0.67 -0.0 3,083,287 - speA b2938 0.67 -0.2 3,083,305 + speA b2938 0.68 -0.3 3,083,305 + speA b2938 0.68 +0.1 3,083,361 + speA b2938 0.71 +0.3 3,083,361 + speA b2938 0.71 +0.5 3,083,365 - speA b2938 0.71 -1.1 3,083,365 - speA b2938 0.71 +1.7 3,083,408 + speA b2938 0.73 -0.0 3,083,416 - speA b2938 0.74 -0.2 3,083,440 - speA b2938 0.75 -2.5 3,083,440 - speA b2938 0.75 +0.5 3,083,452 - speA b2938 0.76 +0.8 3,083,452 - speA b2938 0.76 -0.7 3,083,462 - speA b2938 0.76 -0.3 3,083,575 - speA b2938 0.82 -1.1 3,083,575 - speA b2938 0.82 -0.0 3,083,618 - speA b2938 0.84 -0.2 3,083,677 - speA b2938 0.87 +0.4 3,083,693 + speA b2938 0.88 +0.5 3,083,693 + speA b2938 0.88 +0.4 3,083,761 - -3.1 3,083,772 - -0.5 3,083,802 - -0.1 3,083,823 + +0.6 3,083,834 - +0.6 3,083,834 - +1.3 3,083,872 + -0.1 3,083,874 + -1.0 3,083,880 - +0.5 3,083,880 - -0.7 3,083,911 + +0.1 3,083,985 + yqgB b2939 0.33 +0.8 3,084,042 + yqgB b2939 0.76 -1.3 3,084,150 - -0.1 3,084,153 + -0.2 3,084,166 - -1.3 3,084,181 - -0.3 3,084,227 + +0.2 3,084,242 + yqgC b2940 0.15 -0.6 3,084,259 - yqgC b2940 0.23 +0.4 3,084,259 - yqgC b2940 0.23 -0.1 3,084,259 - yqgC b2940 0.23 +0.2 3,084,285 - yqgC b2940 0.35 -0.4 3,084,345 - yqgC b2940 0.63 +0.2 3,084,471 + -0.4 3,084,632 + -0.8 3,084,632 + -0.7
Or see this region's nucleotide sequence