Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysI and cysJ overlap by 1 nucleotides cysJ and ygcM are separated by 315 nucleotides ygcM and ygcN are separated by 77 nucleotides
b2763: cysI - sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI), at 2,886,409 to 2,888,121
cysI
b2764: cysJ - sulfite reductase, alpha subunit, flavoprotein (NCBI), at 2,888,121 to 2,889,920
cysJ
b2765: ygcM - 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI), at 2,890,236 to 2,890,601
ygcM
b2766: ygcN - orf, hypothetical protein (VIMSS), at 2,890,679 to 2,891,950
ygcN
Position (kb)
2888
2889
2890 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2887.203 kb on - strand, within cysI at 2887.203 kb on - strand, within cysI at 2887.234 kb on - strand, within cysI at 2887.339 kb on - strand, within cysI at 2887.339 kb on - strand, within cysI at 2887.531 kb on - strand, within cysI at 2887.531 kb on - strand, within cysI at 2887.565 kb on + strand, within cysI at 2887.565 kb on + strand, within cysI at 2887.606 kb on + strand, within cysI at 2887.614 kb on - strand, within cysI at 2887.654 kb on + strand, within cysI at 2887.657 kb on - strand, within cysI at 2887.657 kb on - strand, within cysI at 2887.690 kb on - strand, within cysI at 2887.943 kb on + strand, within cysI at 2887.943 kb on + strand, within cysI at 2887.978 kb on - strand at 2887.979 kb on - strand at 2887.986 kb on + strand at 2888.091 kb on + strand at 2888.140 kb on - strand at 2888.145 kb on - strand at 2888.159 kb on + strand at 2888.159 kb on + strand at 2888.301 kb on + strand, within cysJ at 2888.306 kb on + strand, within cysJ at 2888.377 kb on - strand, within cysJ at 2888.377 kb on - strand, within cysJ at 2888.382 kb on - strand, within cysJ at 2888.382 kb on - strand, within cysJ at 2888.417 kb on + strand, within cysJ at 2888.513 kb on - strand, within cysJ at 2888.513 kb on - strand, within cysJ at 2888.515 kb on + strand, within cysJ at 2888.515 kb on + strand, within cysJ at 2888.525 kb on + strand, within cysJ at 2888.525 kb on + strand, within cysJ at 2888.551 kb on - strand, within cysJ at 2888.551 kb on - strand, within cysJ at 2888.648 kb on - strand, within cysJ at 2888.648 kb on - strand, within cysJ at 2888.662 kb on + strand, within cysJ at 2888.662 kb on + strand, within cysJ at 2888.857 kb on + strand, within cysJ at 2888.892 kb on - strand, within cysJ at 2888.928 kb on + strand, within cysJ at 2888.993 kb on - strand, within cysJ at 2888.993 kb on - strand, within cysJ at 2889.056 kb on + strand, within cysJ at 2889.056 kb on + strand, within cysJ at 2889.107 kb on + strand, within cysJ at 2889.114 kb on + strand, within cysJ at 2889.114 kb on + strand, within cysJ at 2889.375 kb on + strand, within cysJ at 2889.375 kb on + strand, within cysJ at 2889.391 kb on - strand, within cysJ at 2889.391 kb on - strand, within cysJ at 2889.481 kb on - strand, within cysJ at 2889.492 kb on - strand, within cysJ at 2889.495 kb on - strand, within cysJ at 2889.495 kb on - strand, within cysJ at 2889.583 kb on - strand, within cysJ at 2889.583 kb on - strand, within cysJ at 2889.804 kb on - strand at 2889.804 kb on - strand at 2889.919 kb on - strand at 2889.919 kb on - strand at 2889.988 kb on + strand at 2889.988 kb on + strand at 2890.285 kb on + strand, within ygcM at 2890.305 kb on - strand, within ygcM at 2890.305 kb on - strand, within ygcM at 2890.344 kb on - strand, within ygcM at 2890.344 kb on - strand, within ygcM at 2890.541 kb on + strand, within ygcM at 2890.541 kb on + strand, within ygcM at 2890.544 kb on - strand, within ygcM at 2890.544 kb on - strand, within ygcM at 2890.551 kb on + strand, within ygcM at 2890.607 kb on - strand at 2890.649 kb on - strand at 2890.820 kb on + strand, within ygcN at 2890.822 kb on + strand, within ygcN at 2890.825 kb on + strand, within ygcN at 2890.830 kb on - strand, within ygcN at 2890.830 kb on - strand, within ygcN at 2890.830 kb on - strand, within ygcN at 2890.830 kb on - strand, within ygcN at 2890.883 kb on - strand, within ygcN at 2890.883 kb on - strand, within ygcN at 2890.885 kb on - strand, within ygcN at 2890.902 kb on + strand, within ygcN
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 2,887,203 - cysI b2763 0.46 -0.2 2,887,203 - cysI b2763 0.46 -2.5 2,887,234 - cysI b2763 0.48 -2.5 2,887,339 - cysI b2763 0.54 -2.8 2,887,339 - cysI b2763 0.54 -1.7 2,887,531 - cysI b2763 0.65 -1.1 2,887,531 - cysI b2763 0.65 -2.1 2,887,565 + cysI b2763 0.67 -0.5 2,887,565 + cysI b2763 0.67 -1.9 2,887,606 + cysI b2763 0.70 -3.5 2,887,614 - cysI b2763 0.70 +0.3 2,887,654 + cysI b2763 0.73 -4.0 2,887,657 - cysI b2763 0.73 -2.1 2,887,657 - cysI b2763 0.73 -1.8 2,887,690 - cysI b2763 0.75 -3.7 2,887,943 + cysI b2763 0.90 -1.8 2,887,943 + cysI b2763 0.90 -3.4 2,887,978 - -3.0 2,887,979 - -3.0 2,887,986 + -2.4 2,888,091 + -1.3 2,888,140 - -2.1 2,888,145 - -3.4 2,888,159 + -1.0 2,888,159 + -1.8 2,888,301 + cysJ b2764 0.10 -2.0 2,888,306 + cysJ b2764 0.10 -2.8 2,888,377 - cysJ b2764 0.14 +0.8 2,888,377 - cysJ b2764 0.14 -1.2 2,888,382 - cysJ b2764 0.14 -1.8 2,888,382 - cysJ b2764 0.14 -3.5 2,888,417 + cysJ b2764 0.16 -2.9 2,888,513 - cysJ b2764 0.22 -0.6 2,888,513 - cysJ b2764 0.22 -2.2 2,888,515 + cysJ b2764 0.22 -0.6 2,888,515 + cysJ b2764 0.22 -2.6 2,888,525 + cysJ b2764 0.22 -2.4 2,888,525 + cysJ b2764 0.22 -2.6 2,888,551 - cysJ b2764 0.24 -2.0 2,888,551 - cysJ b2764 0.24 -0.6 2,888,648 - cysJ b2764 0.29 -1.3 2,888,648 - cysJ b2764 0.29 -4.2 2,888,662 + cysJ b2764 0.30 -0.7 2,888,662 + cysJ b2764 0.30 -0.2 2,888,857 + cysJ b2764 0.41 -4.1 2,888,892 - cysJ b2764 0.43 -2.0 2,888,928 + cysJ b2764 0.45 -3.8 2,888,993 - cysJ b2764 0.48 -2.3 2,888,993 - cysJ b2764 0.48 -1.8 2,889,056 + cysJ b2764 0.52 -2.9 2,889,056 + cysJ b2764 0.52 -3.4 2,889,107 + cysJ b2764 0.55 -2.3 2,889,114 + cysJ b2764 0.55 -1.8 2,889,114 + cysJ b2764 0.55 -2.6 2,889,375 + cysJ b2764 0.70 -3.4 2,889,375 + cysJ b2764 0.70 -0.3 2,889,391 - cysJ b2764 0.71 -3.6 2,889,391 - cysJ b2764 0.71 -2.1 2,889,481 - cysJ b2764 0.76 -3.8 2,889,492 - cysJ b2764 0.76 -2.0 2,889,495 - cysJ b2764 0.76 -1.4 2,889,495 - cysJ b2764 0.76 -0.3 2,889,583 - cysJ b2764 0.81 -3.5 2,889,583 - cysJ b2764 0.81 -3.6 2,889,804 - -2.2 2,889,804 - -2.3 2,889,919 - -2.7 2,889,919 - -1.4 2,889,988 + -1.6 2,889,988 + -3.7 2,890,285 + ygcM b2765 0.13 +0.5 2,890,305 - ygcM b2765 0.19 +0.2 2,890,305 - ygcM b2765 0.19 +0.7 2,890,344 - ygcM b2765 0.30 +0.3 2,890,344 - ygcM b2765 0.30 +1.2 2,890,541 + ygcM b2765 0.83 -0.0 2,890,541 + ygcM b2765 0.83 -1.8 2,890,544 - ygcM b2765 0.84 -0.9 2,890,544 - ygcM b2765 0.84 -1.0 2,890,551 + ygcM b2765 0.86 -0.2 2,890,607 - -0.1 2,890,649 - -0.8 2,890,820 + ygcN b2766 0.11 +0.2 2,890,822 + ygcN b2766 0.11 -0.4 2,890,825 + ygcN b2766 0.11 +0.2 2,890,830 - ygcN b2766 0.12 +0.6 2,890,830 - ygcN b2766 0.12 +1.2 2,890,830 - ygcN b2766 0.12 -0.3 2,890,830 - ygcN b2766 0.12 +0.7 2,890,883 - ygcN b2766 0.16 -0.1 2,890,883 - ygcN b2766 0.16 -0.4 2,890,885 - ygcN b2766 0.16 -0.0 2,890,902 + ygcN b2766 0.18 -1.2
Or see this region's nucleotide sequence