Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysN and cysD are separated by 1 nucleotides cysD and iap are separated by 251 nucleotides
b2751: cysN - sulfate adenylyltransferase subunit 1 (NCBI), at 2,872,014 to 2,873,441
cysN
b2752: cysD - sulfate adenylyltransferase subunit 2 (NCBI), at 2,873,443 to 2,874,351
cysD
b2753: iap - aminopeptidase in alkaline phosphatase isozyme conversion (NCBI), at 2,874,603 to 2,875,640
iap
Position (kb)
2873
2874
2875 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2872.478 kb on - strand, within cysN at 2872.760 kb on + strand, within cysN at 2872.768 kb on - strand, within cysN at 2872.768 kb on - strand, within cysN at 2872.963 kb on + strand, within cysN at 2872.965 kb on + strand, within cysN at 2872.971 kb on - strand, within cysN at 2873.014 kb on + strand, within cysN at 2873.085 kb on - strand, within cysN at 2873.093 kb on + strand, within cysN at 2873.179 kb on - strand, within cysN at 2873.260 kb on + strand, within cysN at 2873.472 kb on + strand at 2873.480 kb on - strand at 2873.480 kb on - strand at 2873.480 kb on - strand at 2873.614 kb on + strand, within cysD at 2873.773 kb on + strand, within cysD at 2873.773 kb on + strand, within cysD at 2873.837 kb on + strand, within cysD at 2873.951 kb on - strand, within cysD at 2873.951 kb on - strand, within cysD at 2873.953 kb on + strand, within cysD at 2874.048 kb on + strand, within cysD at 2874.225 kb on - strand, within cysD at 2874.306 kb on + strand at 2874.325 kb on - strand at 2874.330 kb on - strand at 2874.343 kb on - strand at 2874.362 kb on + strand at 2874.362 kb on + strand at 2874.367 kb on - strand at 2874.394 kb on - strand at 2874.394 kb on - strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.407 kb on - strand at 2874.407 kb on - strand at 2874.407 kb on - strand at 2874.435 kb on + strand at 2874.459 kb on + strand at 2874.499 kb on - strand at 2874.500 kb on + strand at 2874.561 kb on - strand at 2874.561 kb on - strand at 2874.562 kb on - strand at 2874.586 kb on + strand at 2874.620 kb on + strand at 2874.622 kb on + strand at 2874.623 kb on + strand at 2874.623 kb on + strand at 2874.623 kb on + strand at 2874.623 kb on + strand at 2874.628 kb on + strand at 2874.628 kb on - strand at 2874.631 kb on - strand at 2874.631 kb on - strand at 2874.638 kb on + strand at 2874.641 kb on - strand at 2874.641 kb on - strand at 2874.641 kb on - strand at 2874.658 kb on - strand at 2874.666 kb on + strand at 2874.706 kb on - strand at 2874.709 kb on + strand, within iap at 2874.747 kb on + strand, within iap at 2874.747 kb on + strand, within iap at 2874.751 kb on - strand, within iap at 2874.755 kb on - strand, within iap at 2874.755 kb on - strand, within iap at 2874.764 kb on + strand, within iap at 2874.764 kb on + strand, within iap at 2874.784 kb on - strand, within iap at 2874.841 kb on + strand, within iap at 2874.841 kb on + strand, within iap at 2874.845 kb on + strand, within iap at 2874.845 kb on + strand, within iap at 2874.853 kb on - strand, within iap at 2874.866 kb on + strand, within iap at 2874.878 kb on + strand, within iap at 2874.903 kb on - strand, within iap at 2874.915 kb on - strand, within iap at 2874.937 kb on + strand, within iap at 2874.937 kb on + strand, within iap at 2874.946 kb on - strand, within iap at 2874.961 kb on - strand, within iap at 2874.968 kb on - strand, within iap at 2874.969 kb on + strand, within iap at 2875.012 kb on + strand, within iap at 2875.012 kb on + strand, within iap at 2875.020 kb on - strand, within iap at 2875.021 kb on + strand, within iap at 2875.026 kb on + strand, within iap at 2875.026 kb on + strand, within iap at 2875.041 kb on + strand, within iap at 2875.053 kb on + strand, within iap at 2875.081 kb on - strand, within iap at 2875.094 kb on + strand, within iap at 2875.094 kb on + strand, within iap at 2875.097 kb on - strand, within iap at 2875.105 kb on + strand, within iap at 2875.146 kb on + strand, within iap at 2875.189 kb on + strand, within iap at 2875.214 kb on - strand, within iap at 2875.224 kb on - strand at 2875.321 kb on + strand, within iap at 2875.321 kb on + strand, within iap at 2875.321 kb on + strand, within iap at 2875.321 kb on + strand, within iap at 2875.321 kb on + strand, within iap
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 2,872,478 - cysN b2751 0.32 -1.2 2,872,760 + cysN b2751 0.52 -2.3 2,872,768 - cysN b2751 0.53 -0.9 2,872,768 - cysN b2751 0.53 -2.4 2,872,963 + cysN b2751 0.66 +0.1 2,872,965 + cysN b2751 0.67 -1.4 2,872,971 - cysN b2751 0.67 -2.8 2,873,014 + cysN b2751 0.70 -1.9 2,873,085 - cysN b2751 0.75 -2.0 2,873,093 + cysN b2751 0.76 -3.3 2,873,179 - cysN b2751 0.82 -2.8 2,873,260 + cysN b2751 0.87 -1.5 2,873,472 + -1.2 2,873,480 - -1.4 2,873,480 - -1.2 2,873,480 - -0.8 2,873,614 + cysD b2752 0.19 -1.3 2,873,773 + cysD b2752 0.36 -2.0 2,873,773 + cysD b2752 0.36 -0.5 2,873,837 + cysD b2752 0.43 -3.0 2,873,951 - cysD b2752 0.56 -3.0 2,873,951 - cysD b2752 0.56 -2.5 2,873,953 + cysD b2752 0.56 -1.2 2,874,048 + cysD b2752 0.67 -1.3 2,874,225 - cysD b2752 0.86 -1.8 2,874,306 + -1.2 2,874,325 - -1.7 2,874,330 - -0.8 2,874,343 - -1.8 2,874,362 + -2.6 2,874,362 + -1.6 2,874,367 - -0.2 2,874,394 - +0.7 2,874,394 - +0.3 2,874,399 + -1.2 2,874,399 + -1.8 2,874,399 + +0.3 2,874,399 + -1.0 2,874,407 - -0.5 2,874,407 - +0.6 2,874,407 - +0.2 2,874,435 + -1.3 2,874,459 + -0.1 2,874,499 - -0.2 2,874,500 + +0.4 2,874,561 - -1.5 2,874,561 - -1.4 2,874,562 - +0.5 2,874,586 + -0.3 2,874,620 + -0.3 2,874,622 + +0.9 2,874,623 + +0.1 2,874,623 + +0.1 2,874,623 + +0.2 2,874,623 + -0.0 2,874,628 + +0.4 2,874,628 - +1.1 2,874,631 - -0.2 2,874,631 - -0.1 2,874,638 + -0.7 2,874,641 - -0.9 2,874,641 - +0.1 2,874,641 - -0.1 2,874,658 - +0.2 2,874,666 + +0.3 2,874,706 - -0.2 2,874,709 + iap b2753 0.10 +0.7 2,874,747 + iap b2753 0.14 +0.1 2,874,747 + iap b2753 0.14 -0.7 2,874,751 - iap b2753 0.14 -0.3 2,874,755 - iap b2753 0.15 -0.0 2,874,755 - iap b2753 0.15 -0.7 2,874,764 + iap b2753 0.16 -0.7 2,874,764 + iap b2753 0.16 -0.8 2,874,784 - iap b2753 0.17 -0.1 2,874,841 + iap b2753 0.23 -0.0 2,874,841 + iap b2753 0.23 +0.3 2,874,845 + iap b2753 0.23 -1.1 2,874,845 + iap b2753 0.23 -1.6 2,874,853 - iap b2753 0.24 +1.2 2,874,866 + iap b2753 0.25 -0.3 2,874,878 + iap b2753 0.26 -0.5 2,874,903 - iap b2753 0.29 +2.3 2,874,915 - iap b2753 0.30 +1.0 2,874,937 + iap b2753 0.32 +0.3 2,874,937 + iap b2753 0.32 +0.5 2,874,946 - iap b2753 0.33 -0.5 2,874,961 - iap b2753 0.34 -0.6 2,874,968 - iap b2753 0.35 +0.6 2,874,969 + iap b2753 0.35 +0.2 2,875,012 + iap b2753 0.39 -1.6 2,875,012 + iap b2753 0.39 +0.3 2,875,020 - iap b2753 0.40 +0.1 2,875,021 + iap b2753 0.40 -0.3 2,875,026 + iap b2753 0.41 +0.3 2,875,026 + iap b2753 0.41 -0.4 2,875,041 + iap b2753 0.42 +0.1 2,875,053 + iap b2753 0.43 -0.2 2,875,081 - iap b2753 0.46 +1.4 2,875,094 + iap b2753 0.47 +0.2 2,875,094 + iap b2753 0.47 +0.2 2,875,097 - iap b2753 0.48 +0.7 2,875,105 + iap b2753 0.48 +0.2 2,875,146 + iap b2753 0.52 -0.0 2,875,189 + iap b2753 0.56 -0.3 2,875,214 - iap b2753 0.59 -0.7 2,875,224 - +1.7 2,875,321 + iap b2753 0.69 -0.1 2,875,321 + iap b2753 0.69 -0.6 2,875,321 + iap b2753 0.69 +1.3 2,875,321 + iap b2753 0.69 -0.4 2,875,321 + iap b2753 0.69 -0.0
Or see this region's nucleotide sequence