Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygbE and cysC are separated by 49 nucleotides cysC and cysN overlap by 1 nucleotides cysN and cysD are separated by 1 nucleotides
b2749: ygbE - conserved inner membrane protein (NCBI), at 2,871,036 to 2,871,359
ygbE
b2750: cysC - adenylylsulfate kinase (NCBI), at 2,871,409 to 2,872,014
cysC
b2751: cysN - sulfate adenylyltransferase subunit 1 (NCBI), at 2,872,014 to 2,873,441
cysN
b2752: cysD - sulfate adenylyltransferase subunit 2 (NCBI), at 2,873,443 to 2,874,351
cysD
Position (kb)
2872
2873
2874 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2871.097 kb on + strand, within ygbE at 2871.140 kb on - strand, within ygbE at 2871.145 kb on - strand, within ygbE at 2871.145 kb on - strand, within ygbE at 2871.149 kb on + strand, within ygbE at 2871.197 kb on - strand, within ygbE at 2871.197 kb on - strand, within ygbE at 2871.197 kb on - strand, within ygbE at 2871.207 kb on - strand, within ygbE at 2871.207 kb on - strand, within ygbE at 2871.248 kb on + strand, within ygbE at 2871.285 kb on - strand, within ygbE at 2871.290 kb on + strand, within ygbE at 2871.311 kb on + strand, within ygbE at 2871.391 kb on + strand at 2871.421 kb on - strand at 2871.534 kb on - strand, within cysC at 2871.692 kb on - strand, within cysC at 2871.703 kb on + strand, within cysC at 2871.807 kb on - strand, within cysC at 2871.807 kb on - strand, within cysC at 2871.866 kb on + strand, within cysC at 2871.866 kb on + strand, within cysC at 2871.907 kb on - strand, within cysC at 2871.911 kb on - strand, within cysC at 2871.919 kb on + strand, within cysC at 2871.952 kb on + strand, within cysC at 2871.953 kb on - strand, within cysC at 2871.966 kb on - strand at 2871.968 kb on - strand at 2872.057 kb on - strand at 2872.264 kb on + strand, within cysN at 2872.298 kb on + strand, within cysN at 2872.478 kb on - strand, within cysN at 2872.760 kb on + strand, within cysN at 2872.768 kb on - strand, within cysN at 2872.768 kb on - strand, within cysN at 2872.963 kb on + strand, within cysN at 2872.965 kb on + strand, within cysN at 2872.971 kb on - strand, within cysN at 2873.014 kb on + strand, within cysN at 2873.085 kb on - strand, within cysN at 2873.093 kb on + strand, within cysN at 2873.179 kb on - strand, within cysN at 2873.260 kb on + strand, within cysN at 2873.472 kb on + strand at 2873.480 kb on - strand at 2873.480 kb on - strand at 2873.480 kb on - strand at 2873.614 kb on + strand, within cysD at 2873.773 kb on + strand, within cysD at 2873.773 kb on + strand, within cysD at 2873.837 kb on + strand, within cysD at 2873.951 kb on - strand, within cysD at 2873.951 kb on - strand, within cysD at 2873.953 kb on + strand, within cysD at 2874.048 kb on + strand, within cysD at 2874.225 kb on - strand, within cysD at 2874.306 kb on + strand at 2874.325 kb on - strand at 2874.330 kb on - strand at 2874.343 kb on - strand at 2874.362 kb on + strand at 2874.362 kb on + strand at 2874.367 kb on - strand at 2874.394 kb on - strand at 2874.394 kb on - strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.407 kb on - strand at 2874.407 kb on - strand at 2874.407 kb on - strand at 2874.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 2,871,097 + ygbE b2749 0.19 -1.0 2,871,140 - ygbE b2749 0.32 -0.5 2,871,145 - ygbE b2749 0.34 -0.7 2,871,145 - ygbE b2749 0.34 -0.2 2,871,149 + ygbE b2749 0.35 -0.8 2,871,197 - ygbE b2749 0.50 -0.4 2,871,197 - ygbE b2749 0.50 +2.1 2,871,197 - ygbE b2749 0.50 -0.1 2,871,207 - ygbE b2749 0.53 -0.6 2,871,207 - ygbE b2749 0.53 -0.7 2,871,248 + ygbE b2749 0.65 -1.5 2,871,285 - ygbE b2749 0.77 -0.3 2,871,290 + ygbE b2749 0.78 -1.1 2,871,311 + ygbE b2749 0.85 -0.9 2,871,391 + -1.1 2,871,421 - -0.8 2,871,534 - cysC b2750 0.21 -4.1 2,871,692 - cysC b2750 0.47 -3.0 2,871,703 + cysC b2750 0.49 -1.6 2,871,807 - cysC b2750 0.66 -2.0 2,871,807 - cysC b2750 0.66 -2.6 2,871,866 + cysC b2750 0.75 -2.9 2,871,866 + cysC b2750 0.75 -1.9 2,871,907 - cysC b2750 0.82 -1.9 2,871,911 - cysC b2750 0.83 -2.8 2,871,919 + cysC b2750 0.84 -3.3 2,871,952 + cysC b2750 0.90 -2.7 2,871,953 - cysC b2750 0.90 -1.8 2,871,966 - -2.6 2,871,968 - -1.9 2,872,057 - -0.6 2,872,264 + cysN b2751 0.18 -2.8 2,872,298 + cysN b2751 0.20 -2.4 2,872,478 - cysN b2751 0.32 -1.2 2,872,760 + cysN b2751 0.52 -2.3 2,872,768 - cysN b2751 0.53 -0.9 2,872,768 - cysN b2751 0.53 -2.4 2,872,963 + cysN b2751 0.66 +0.1 2,872,965 + cysN b2751 0.67 -1.4 2,872,971 - cysN b2751 0.67 -2.8 2,873,014 + cysN b2751 0.70 -1.9 2,873,085 - cysN b2751 0.75 -2.0 2,873,093 + cysN b2751 0.76 -3.3 2,873,179 - cysN b2751 0.82 -2.8 2,873,260 + cysN b2751 0.87 -1.5 2,873,472 + -1.2 2,873,480 - -1.4 2,873,480 - -1.2 2,873,480 - -0.8 2,873,614 + cysD b2752 0.19 -1.3 2,873,773 + cysD b2752 0.36 -2.0 2,873,773 + cysD b2752 0.36 -0.5 2,873,837 + cysD b2752 0.43 -3.0 2,873,951 - cysD b2752 0.56 -3.0 2,873,951 - cysD b2752 0.56 -2.5 2,873,953 + cysD b2752 0.56 -1.2 2,874,048 + cysD b2752 0.67 -1.3 2,874,225 - cysD b2752 0.86 -1.8 2,874,306 + -1.2 2,874,325 - -1.7 2,874,330 - -0.8 2,874,343 - -1.8 2,874,362 + -2.6 2,874,362 + -1.6 2,874,367 - -0.2 2,874,394 - +0.7 2,874,394 - +0.3 2,874,399 + -1.2 2,874,399 + -1.8 2,874,399 + +0.3 2,874,399 + -1.0 2,874,407 - -0.5 2,874,407 - +0.6 2,874,407 - +0.2 2,874,435 + -1.3
Or see this region's nucleotide sequence