Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcV and sixA are separated by 680 nucleotides sixA and yfcX are separated by 202 nucleotides
b2339: yfcV - predicted fimbrial-like adhesin protein (NCBI), at 2,453,105 to 2,453,668
yfcV
b2340: sixA - phosphohistidine phosphatase (NCBI), at 2,454,349 to 2,454,834
sixA
b2341: yfcX - fused enoyl-CoA hydratase and epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase (NCBI), at 2,455,037 to 2,457,181
yfcX
Position (kb)
2454
2455 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2453.383 kb on + strand, within yfcV at 2453.383 kb on + strand, within yfcV at 2453.385 kb on + strand, within yfcV at 2453.385 kb on + strand, within yfcV at 2453.389 kb on + strand, within yfcV at 2453.391 kb on - strand, within yfcV at 2453.397 kb on - strand, within yfcV at 2453.397 kb on - strand, within yfcV at 2453.404 kb on + strand, within yfcV at 2453.458 kb on + strand, within yfcV at 2453.493 kb on + strand, within yfcV at 2453.515 kb on + strand, within yfcV at 2453.532 kb on - strand, within yfcV at 2453.535 kb on + strand, within yfcV at 2453.538 kb on + strand, within yfcV at 2453.549 kb on + strand, within yfcV at 2453.549 kb on + strand, within yfcV at 2453.598 kb on + strand, within yfcV at 2453.598 kb on + strand, within yfcV at 2453.635 kb on + strand at 2454.088 kb on - strand at 2454.088 kb on - strand at 2454.178 kb on - strand at 2454.185 kb on - strand at 2454.201 kb on - strand at 2454.201 kb on - strand at 2454.216 kb on - strand at 2454.268 kb on - strand at 2454.275 kb on - strand at 2454.275 kb on - strand at 2454.280 kb on + strand at 2454.290 kb on - strand at 2454.290 kb on - strand at 2454.295 kb on - strand at 2454.295 kb on - strand at 2454.330 kb on - strand at 2454.375 kb on - strand at 2454.375 kb on - strand at 2454.389 kb on - strand at 2454.476 kb on + strand, within sixA at 2454.476 kb on + strand, within sixA at 2454.482 kb on - strand, within sixA at 2454.509 kb on + strand, within sixA at 2454.520 kb on + strand, within sixA at 2454.535 kb on - strand, within sixA at 2454.535 kb on - strand, within sixA at 2454.550 kb on - strand, within sixA at 2454.584 kb on - strand, within sixA at 2454.584 kb on - strand, within sixA at 2455.035 kb on + strand at 2455.071 kb on + strand at 2455.071 kb on + strand at 2455.084 kb on + strand at 2455.185 kb on + strand at 2455.185 kb on + strand at 2455.262 kb on + strand, within yfcX at 2455.268 kb on + strand, within yfcX at 2455.321 kb on + strand, within yfcX at 2455.414 kb on - strand, within yfcX at 2455.418 kb on - strand, within yfcX at 2455.587 kb on - strand, within yfcX at 2455.620 kb on + strand, within yfcX at 2455.796 kb on - strand, within yfcX
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 2,453,383 + yfcV b2339 0.49 -0.0 2,453,383 + yfcV b2339 0.49 -0.7 2,453,385 + yfcV b2339 0.50 -0.6 2,453,385 + yfcV b2339 0.50 +0.6 2,453,389 + yfcV b2339 0.50 +1.2 2,453,391 - yfcV b2339 0.51 +1.0 2,453,397 - yfcV b2339 0.52 +2.0 2,453,397 - yfcV b2339 0.52 +2.0 2,453,404 + yfcV b2339 0.53 +1.0 2,453,458 + yfcV b2339 0.63 +0.6 2,453,493 + yfcV b2339 0.69 -0.7 2,453,515 + yfcV b2339 0.73 +1.0 2,453,532 - yfcV b2339 0.76 +1.0 2,453,535 + yfcV b2339 0.76 +1.0 2,453,538 + yfcV b2339 0.77 +1.6 2,453,549 + yfcV b2339 0.79 -0.0 2,453,549 + yfcV b2339 0.79 -0.6 2,453,598 + yfcV b2339 0.87 -0.6 2,453,598 + yfcV b2339 0.87 +1.2 2,453,635 + -0.4 2,454,088 - -0.8 2,454,088 - +2.0 2,454,178 - -0.3 2,454,185 - -0.6 2,454,201 - +0.4 2,454,201 - +1.1 2,454,216 - +2.0 2,454,268 - -0.0 2,454,275 - +2.0 2,454,275 - -0.6 2,454,280 + -0.5 2,454,290 - +2.0 2,454,290 - -0.6 2,454,295 - +1.0 2,454,295 - -2.0 2,454,330 - -1.8 2,454,375 - +0.7 2,454,375 - -0.5 2,454,389 - -0.8 2,454,476 + sixA b2340 0.26 -0.6 2,454,476 + sixA b2340 0.26 +1.0 2,454,482 - sixA b2340 0.27 -1.5 2,454,509 + sixA b2340 0.33 -0.6 2,454,520 + sixA b2340 0.35 -2.0 2,454,535 - sixA b2340 0.38 +0.4 2,454,535 - sixA b2340 0.38 -2.2 2,454,550 - sixA b2340 0.41 -0.7 2,454,584 - sixA b2340 0.48 -1.3 2,454,584 - sixA b2340 0.48 -0.2 2,455,035 + -0.7 2,455,071 + -1.1 2,455,071 + -1.6 2,455,084 + -0.1 2,455,185 + +0.2 2,455,185 + +0.1 2,455,262 + yfcX b2341 0.10 -0.0 2,455,268 + yfcX b2341 0.11 -0.2 2,455,321 + yfcX b2341 0.13 -0.6 2,455,414 - yfcX b2341 0.18 -0.7 2,455,418 - yfcX b2341 0.18 +0.3 2,455,587 - yfcX b2341 0.26 -0.1 2,455,620 + yfcX b2341 0.27 -0.7 2,455,796 - yfcX b2341 0.35 -0.1
Or see this region's nucleotide sequence