Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebA and yebL are separated by 15 nucleotides yebL and znuC are separated by 78 nucleotides znuC and znuB overlap by 4 nucleotides
b1856: yebA - orf, hypothetical protein (VIMSS), at 1,938,337 to 1,939,659
yebA
b1857: yebL - putative adhesin (VIMSS), at 1,939,675 to 1,940,607
yebL
b1858: znuC - high-affinity zinc transporter ATPase (NCBI), at 1,940,686 to 1,941,441
znuC
b1859: znuB - high-affinity zinc transporter membrane component (NCBI), at 1,941,438 to 1,942,223
znuB
Position (kb)
1939
1940
1941 Strain fitness (log2 ratio)
-2
-1
0
1 at 1938.726 kb on - strand, within yebA at 1938.822 kb on + strand, within yebA at 1938.822 kb on + strand, within yebA at 1939.004 kb on - strand, within yebA at 1939.128 kb on - strand, within yebA at 1939.179 kb on - strand, within yebA at 1939.204 kb on - strand, within yebA at 1939.241 kb on - strand, within yebA at 1939.243 kb on - strand, within yebA at 1939.287 kb on + strand, within yebA at 1939.301 kb on + strand, within yebA at 1939.301 kb on + strand, within yebA at 1939.423 kb on + strand, within yebA at 1939.440 kb on - strand, within yebA at 1939.476 kb on - strand, within yebA at 1939.652 kb on - strand at 1939.694 kb on + strand at 1939.813 kb on + strand, within yebL at 1939.826 kb on + strand, within yebL at 1939.826 kb on + strand, within yebL at 1939.834 kb on - strand, within yebL at 1939.834 kb on - strand, within yebL at 1939.834 kb on - strand, within yebL at 1940.017 kb on - strand, within yebL at 1940.017 kb on - strand, within yebL at 1940.087 kb on - strand, within yebL at 1940.087 kb on - strand, within yebL at 1940.238 kb on - strand, within yebL at 1940.238 kb on - strand, within yebL at 1940.554 kb on + strand at 1940.831 kb on - strand, within znuC at 1940.831 kb on - strand, within znuC at 1940.844 kb on + strand, within znuC at 1940.964 kb on + strand, within znuC at 1940.970 kb on - strand, within znuC at 1940.970 kb on - strand, within znuC at 1940.972 kb on - strand, within znuC at 1940.972 kb on - strand, within znuC at 1941.053 kb on - strand, within znuC at 1941.053 kb on - strand, within znuC at 1941.081 kb on - strand, within znuC at 1941.195 kb on + strand, within znuC at 1941.208 kb on - strand, within znuC at 1941.228 kb on + strand, within znuC at 1941.385 kb on - strand at 1941.492 kb on - strand at 1941.587 kb on + strand, within znuB at 1941.587 kb on + strand, within znuB at 1941.589 kb on + strand, within znuB
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 1,938,726 - yebA b1856 0.29 -2.2 1,938,822 + yebA b1856 0.37 +0.6 1,938,822 + yebA b1856 0.37 +1.2 1,939,004 - yebA b1856 0.50 -0.0 1,939,128 - yebA b1856 0.60 +0.4 1,939,179 - yebA b1856 0.64 -0.7 1,939,204 - yebA b1856 0.66 +0.2 1,939,241 - yebA b1856 0.68 +0.1 1,939,243 - yebA b1856 0.68 -0.4 1,939,287 + yebA b1856 0.72 +0.2 1,939,301 + yebA b1856 0.73 -1.3 1,939,301 + yebA b1856 0.73 -0.2 1,939,423 + yebA b1856 0.82 -0.5 1,939,440 - yebA b1856 0.83 +0.5 1,939,476 - yebA b1856 0.86 +0.3 1,939,652 - -0.2 1,939,694 + +0.8 1,939,813 + yebL b1857 0.15 -1.4 1,939,826 + yebL b1857 0.16 -1.9 1,939,826 + yebL b1857 0.16 +0.6 1,939,834 - yebL b1857 0.17 -0.6 1,939,834 - yebL b1857 0.17 -0.8 1,939,834 - yebL b1857 0.17 -1.0 1,940,017 - yebL b1857 0.37 -1.8 1,940,017 - yebL b1857 0.37 -0.8 1,940,087 - yebL b1857 0.44 -1.9 1,940,087 - yebL b1857 0.44 -1.1 1,940,238 - yebL b1857 0.60 -0.6 1,940,238 - yebL b1857 0.60 -0.9 1,940,554 + -1.6 1,940,831 - znuC b1858 0.19 -0.8 1,940,831 - znuC b1858 0.19 -1.1 1,940,844 + znuC b1858 0.21 -0.9 1,940,964 + znuC b1858 0.37 -1.5 1,940,970 - znuC b1858 0.38 +0.6 1,940,970 - znuC b1858 0.38 -1.3 1,940,972 - znuC b1858 0.38 -1.7 1,940,972 - znuC b1858 0.38 -1.2 1,941,053 - znuC b1858 0.49 -0.8 1,941,053 - znuC b1858 0.49 -0.6 1,941,081 - znuC b1858 0.52 -0.1 1,941,195 + znuC b1858 0.67 -0.7 1,941,208 - znuC b1858 0.69 -2.2 1,941,228 + znuC b1858 0.72 -0.2 1,941,385 - -0.1 1,941,492 - -0.5 1,941,587 + znuB b1859 0.19 -0.6 1,941,587 + znuB b1859 0.19 -0.6 1,941,589 + znuB b1859 0.19 -0.1
Or see this region's nucleotide sequence