Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydgD and mdtI are separated by 38 nucleotides mdtI and mdtJ overlap by 14 nucleotides mdtJ and ydgG are separated by 411 nucleotides
b1598: ydgD - predicted peptidase (NCBI), at 1,669,984 to 1,670,805
ydgD
b1599: mdtI - multidrug efflux system transporter (NCBI), at 1,670,844 to 1,671,173
mdtI
b1600: mdtJ - multidrug efflux system transporter (NCBI), at 1,671,160 to 1,671,525
mdtJ
b1601: ydgG - predicted inner membrane protein (NCBI), at 1,671,937 to 1,672,971
ydgG
Position (kb)
1671
1672 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1670.161 kb on - strand, within ydgD at 1670.248 kb on + strand, within ydgD at 1670.337 kb on - strand, within ydgD at 1670.575 kb on + strand, within ydgD at 1670.673 kb on - strand, within ydgD at 1671.156 kb on - strand at 1671.156 kb on - strand at 1671.165 kb on - strand at 1671.182 kb on - strand at 1671.186 kb on + strand at 1671.251 kb on - strand, within mdtJ at 1671.253 kb on - strand, within mdtJ at 1671.346 kb on + strand, within mdtJ at 1671.348 kb on - strand, within mdtJ at 1671.348 kb on - strand, within mdtJ at 1671.354 kb on - strand, within mdtJ at 1671.354 kb on - strand, within mdtJ at 1671.355 kb on - strand, within mdtJ at 1671.449 kb on - strand, within mdtJ at 1671.466 kb on - strand, within mdtJ at 1671.466 kb on - strand, within mdtJ at 1671.474 kb on - strand, within mdtJ at 1671.525 kb on - strand at 1671.595 kb on - strand at 1671.615 kb on - strand at 1671.653 kb on - strand at 1671.653 kb on - strand at 1671.653 kb on - strand at 1671.653 kb on - strand at 1671.665 kb on + strand at 1671.761 kb on - strand at 1671.761 kb on - strand at 1671.761 kb on - strand at 1671.800 kb on - strand at 1671.800 kb on - strand at 1671.927 kb on + strand at 1671.935 kb on + strand at 1671.941 kb on - strand at 1671.943 kb on - strand at 1672.027 kb on + strand at 1672.082 kb on - strand, within ydgG at 1672.082 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.102 kb on - strand, within ydgG at 1672.106 kb on + strand, within ydgG at 1672.151 kb on - strand, within ydgG at 1672.157 kb on - strand, within ydgG at 1672.291 kb on - strand, within ydgG at 1672.365 kb on + strand, within ydgG at 1672.365 kb on + strand, within ydgG at 1672.413 kb on + strand, within ydgG at 1672.481 kb on + strand, within ydgG
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 1,670,161 - ydgD b1598 0.22 -0.4 1,670,248 + ydgD b1598 0.32 -0.4 1,670,337 - ydgD b1598 0.43 -0.2 1,670,575 + ydgD b1598 0.72 -0.0 1,670,673 - ydgD b1598 0.84 -0.2 1,671,156 - +0.1 1,671,156 - +0.2 1,671,165 - +0.5 1,671,182 - +0.1 1,671,186 + +0.6 1,671,251 - mdtJ b1600 0.25 +0.2 1,671,253 - mdtJ b1600 0.25 +1.2 1,671,346 + mdtJ b1600 0.51 +1.8 1,671,348 - mdtJ b1600 0.51 +0.2 1,671,348 - mdtJ b1600 0.51 +0.8 1,671,354 - mdtJ b1600 0.53 +0.5 1,671,354 - mdtJ b1600 0.53 +0.8 1,671,355 - mdtJ b1600 0.53 -2.0 1,671,449 - mdtJ b1600 0.79 +1.8 1,671,466 - mdtJ b1600 0.84 -0.2 1,671,466 - mdtJ b1600 0.84 -0.5 1,671,474 - mdtJ b1600 0.86 +0.8 1,671,525 - +1.8 1,671,595 - +0.0 1,671,615 - +0.2 1,671,653 - -0.5 1,671,653 - +0.0 1,671,653 - +0.9 1,671,653 - -0.3 1,671,665 + +1.8 1,671,761 - -0.9 1,671,761 - -0.0 1,671,761 - +0.5 1,671,800 - +0.9 1,671,800 - +1.2 1,671,927 + +1.8 1,671,935 + +1.8 1,671,941 - +0.2 1,671,943 - +1.8 1,672,027 + -0.8 1,672,082 - ydgG b1601 0.14 +0.4 1,672,082 - ydgG b1601 0.14 +0.1 1,672,095 - ydgG b1601 0.15 +1.2 1,672,095 - ydgG b1601 0.15 +0.8 1,672,095 - ydgG b1601 0.15 +1.2 1,672,102 - ydgG b1601 0.16 +0.7 1,672,106 + ydgG b1601 0.16 -0.4 1,672,151 - ydgG b1601 0.21 +0.7 1,672,157 - ydgG b1601 0.21 -0.4 1,672,291 - ydgG b1601 0.34 +0.6 1,672,365 + ydgG b1601 0.41 -0.6 1,672,365 + ydgG b1601 0.41 +0.1 1,672,413 + ydgG b1601 0.46 -0.9 1,672,481 + ydgG b1601 0.53 -1.0
Or see this region's nucleotide sequence