Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycbK and ycbL are separated by 26 nucleotides ycbL and aspC are separated by 221 nucleotides aspC and ompF are separated by 184 nucleotides
b0926: ycbK - hypothetical protein (NCBI), at 982,298 to 982,846
ycbK
b0927: ycbL - predicted metal-binding enzyme (NCBI), at 982,873 to 983,520
ycbL
b0928: aspC - aspartate aminotransferase, PLP-dependent (NCBI), at 983,742 to 984,932
aspC
b0929: ompF - outer membrane porin 1a (Ia;b;F) (NCBI), at 985,117 to 986,205
ompF
Position (kb)
983
984
985 Strain fitness (log2 ratio)
-2
-1
0
1 at 982.765 kb on - strand, within ycbK at 982.779 kb on + strand, within ycbK at 982.790 kb on - strand, within ycbK at 982.792 kb on - strand at 982.818 kb on - strand at 982.850 kb on - strand at 983.009 kb on + strand, within ycbL at 983.020 kb on + strand, within ycbL at 983.020 kb on + strand, within ycbL at 983.050 kb on - strand, within ycbL at 983.050 kb on - strand, within ycbL at 983.052 kb on - strand, within ycbL at 983.052 kb on - strand, within ycbL at 983.423 kb on - strand, within ycbL at 983.522 kb on - strand at 983.586 kb on - strand at 983.691 kb on - strand at 983.786 kb on - strand at 983.786 kb on - strand at 983.884 kb on + strand, within aspC at 983.983 kb on - strand, within aspC at 984.232 kb on - strand, within aspC at 984.326 kb on + strand, within aspC at 984.365 kb on - strand, within aspC at 984.402 kb on + strand, within aspC at 984.402 kb on + strand, within aspC at 984.535 kb on - strand, within aspC at 984.560 kb on + strand, within aspC at 984.560 kb on + strand, within aspC at 984.633 kb on + strand, within aspC at 984.633 kb on + strand, within aspC at 984.683 kb on - strand, within aspC at 984.792 kb on + strand, within aspC at 984.939 kb on + strand at 984.942 kb on + strand at 984.944 kb on + strand at 984.975 kb on + strand at 985.123 kb on - strand at 985.169 kb on + strand at 985.240 kb on + strand, within ompF at 985.259 kb on - strand, within ompF at 985.483 kb on + strand, within ompF at 985.548 kb on - strand, within ompF at 985.548 kb on - strand, within ompF at 985.682 kb on + strand, within ompF at 985.682 kb on + strand, within ompF at 985.805 kb on + strand, within ompF at 985.813 kb on - strand, within ompF at 985.880 kb on - strand, within ompF
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 982,765 - ycbK b0926 0.85 +1.2 982,779 + ycbK b0926 0.88 +0.6 982,790 - ycbK b0926 0.90 +1.5 982,792 - +0.9 982,818 - +0.4 982,850 - +0.2 983,009 + ycbL b0927 0.21 -0.3 983,020 + ycbL b0927 0.23 -2.5 983,020 + ycbL b0927 0.23 +0.5 983,050 - ycbL b0927 0.27 +0.1 983,050 - ycbL b0927 0.27 -0.3 983,052 - ycbL b0927 0.28 +0.5 983,052 - ycbL b0927 0.28 +0.2 983,423 - ycbL b0927 0.85 +0.3 983,522 - -0.5 983,586 - -0.2 983,691 - +0.3 983,786 - +0.2 983,786 - +1.0 983,884 + aspC b0928 0.12 -1.5 983,983 - aspC b0928 0.20 -1.5 984,232 - aspC b0928 0.41 -0.2 984,326 + aspC b0928 0.49 -0.9 984,365 - aspC b0928 0.52 -1.6 984,402 + aspC b0928 0.55 -1.3 984,402 + aspC b0928 0.55 -2.3 984,535 - aspC b0928 0.67 -2.4 984,560 + aspC b0928 0.69 +0.1 984,560 + aspC b0928 0.69 -2.0 984,633 + aspC b0928 0.75 -1.4 984,633 + aspC b0928 0.75 -1.3 984,683 - aspC b0928 0.79 -1.1 984,792 + aspC b0928 0.88 -1.1 984,939 + -0.5 984,942 + -0.5 984,944 + -1.4 984,975 + -0.4 985,123 - +0.5 985,169 + +0.1 985,240 + ompF b0929 0.11 +0.5 985,259 - ompF b0929 0.13 -1.2 985,483 + ompF b0929 0.34 +0.2 985,548 - ompF b0929 0.40 -0.1 985,548 - ompF b0929 0.40 -0.5 985,682 + ompF b0929 0.52 +1.2 985,682 + ompF b0929 0.52 +0.1 985,805 + ompF b0929 0.63 -0.8 985,813 - ompF b0929 0.64 -0.3 985,880 - ompF b0929 0.70 -0.3
Or see this region's nucleotide sequence