Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trxB and lrp are separated by 544 nucleotides lrp and ftsK are separated by 134 nucleotides
b0888: trxB - thioredoxin reductase, FAD/NAD(P)-binding (NCBI), at 930,308 to 931,273
trxB
b0889: lrp - DNA-binding transcriptional dual regulator, leucine-binding (NCBI), at 931,818 to 932,312
lrp
b0890: ftsK - DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI), at 932,447 to 936,436
ftsK
Position (kb)
931
932
933 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 930.848 kb on - strand, within trxB at 930.848 kb on - strand, within trxB at 930.863 kb on + strand, within trxB at 930.913 kb on - strand, within trxB at 930.939 kb on + strand, within trxB at 931.019 kb on + strand, within trxB at 931.137 kb on - strand, within trxB at 931.193 kb on - strand at 931.215 kb on - strand at 931.327 kb on - strand at 931.327 kb on - strand at 931.379 kb on + strand at 931.411 kb on + strand at 931.435 kb on + strand at 931.641 kb on - strand at 931.641 kb on - strand at 931.721 kb on - strand at 931.764 kb on - strand at 931.764 kb on - strand at 931.801 kb on + strand at 931.856 kb on - strand at 931.864 kb on + strand at 931.937 kb on + strand, within lrp at 932.002 kb on - strand, within lrp at 932.002 kb on - strand, within lrp at 932.004 kb on - strand, within lrp at 932.066 kb on - strand, within lrp at 932.078 kb on + strand, within lrp at 932.331 kb on - strand at 932.331 kb on - strand at 932.353 kb on - strand at 932.385 kb on + strand at 932.385 kb on + strand at 932.385 kb on + strand at 932.430 kb on + strand at 932.430 kb on + strand at 932.614 kb on + strand at 933.053 kb on + strand, within ftsK at 933.095 kb on + strand, within ftsK at 933.102 kb on - strand, within ftsK at 933.104 kb on + strand, within ftsK
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 930,848 - trxB b0888 0.56 +0.5 930,848 - trxB b0888 0.56 -1.3 930,863 + trxB b0888 0.57 -0.1 930,913 - trxB b0888 0.63 -1.7 930,939 + trxB b0888 0.65 -0.6 931,019 + trxB b0888 0.74 +0.7 931,137 - trxB b0888 0.86 -1.5 931,193 - +0.7 931,215 - -1.6 931,327 - -1.1 931,327 - -1.0 931,379 + -0.1 931,411 + +0.6 931,435 + -0.0 931,641 - -2.0 931,641 - -0.6 931,721 - -2.4 931,764 - -2.9 931,764 - -1.3 931,801 + +0.8 931,856 - -1.0 931,864 + -3.3 931,937 + lrp b0889 0.24 -1.8 932,002 - lrp b0889 0.37 -2.0 932,002 - lrp b0889 0.37 -0.7 932,004 - lrp b0889 0.38 -3.6 932,066 - lrp b0889 0.50 -1.7 932,078 + lrp b0889 0.53 -0.4 932,331 - -0.1 932,331 - -2.0 932,353 - -1.7 932,385 + -0.3 932,385 + -0.8 932,385 + -0.1 932,430 + -0.3 932,430 + +0.2 932,614 + +0.4 933,053 + ftsK b0890 0.15 -1.1 933,095 + ftsK b0890 0.16 +0.9 933,102 - ftsK b0890 0.16 +0.3 933,104 + ftsK b0890 0.16 +1.9
Or see this region's nucleotide sequence