Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaK and dnaJ are separated by 88 nucleotides dnaJ and insL-1 are separated by 146 nucleotides
b0014: dnaK - molecular chaperone DnaK (NCBI), at 12,163 to 14,079
dnaK
b0015: dnaJ - chaperone Hsp40, co-chaperone with DnaK (NCBI), at 14,168 to 15,298
dnaJ
b0016: insL-1 - IS186/IS421 transposase (NCBI), at 15,445 to 16,557
insL-1
Position (kb) 14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 13.673 kb on + strand, within dnaK at 13.673 kb on + strand, within dnaK at 13.673 kb on + strand, within dnaK at 13.859 kb on + strand, within dnaK at 13.894 kb on + strand at 13.894 kb on + strand at 14.116 kb on + strand at 14.145 kb on + strand at 14.245 kb on - strand at 14.260 kb on - strand at 14.264 kb on + strand at 14.264 kb on + strand at 14.325 kb on + strand, within dnaJ at 14.371 kb on + strand, within dnaJ at 14.374 kb on - strand, within dnaJ at 14.508 kb on + strand, within dnaJ at 14.508 kb on + strand, within dnaJ at 14.521 kb on + strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.596 kb on + strand, within dnaJ at 14.596 kb on + strand, within dnaJ at 14.596 kb on - strand, within dnaJ at 14.627 kb on - strand, within dnaJ at 14.664 kb on + strand, within dnaJ at 14.664 kb on + strand, within dnaJ at 14.672 kb on - strand, within dnaJ at 14.682 kb on - strand, within dnaJ at 14.702 kb on + strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.745 kb on + strand, within dnaJ at 14.781 kb on - strand, within dnaJ at 14.781 kb on - strand, within dnaJ at 14.829 kb on + strand, within dnaJ at 14.829 kb on + strand, within dnaJ at 14.842 kb on - strand, within dnaJ at 14.842 kb on - strand, within dnaJ at 14.859 kb on + strand, within dnaJ at 14.859 kb on + strand, within dnaJ at 14.923 kb on + strand, within dnaJ at 14.959 kb on - strand, within dnaJ at 15.087 kb on + strand, within dnaJ at 15.087 kb on + strand, within dnaJ at 15.111 kb on + strand, within dnaJ at 15.111 kb on + strand, within dnaJ at 15.111 kb on + strand, within dnaJ at 15.143 kb on + strand, within dnaJ at 15.164 kb on - strand, within dnaJ at 15.235 kb on - strand at 15.235 kb on - strand at 15.289 kb on + strand at 15.389 kb on - strand at 15.410 kb on - strand at 15.439 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 13,673 + dnaK b0014 0.79 +0.4 13,673 + dnaK b0014 0.79 -1.1 13,673 + dnaK b0014 0.79 +0.2 13,859 + dnaK b0014 0.88 -1.9 13,894 + +0.3 13,894 + -0.2 14,116 + -0.9 14,145 + -0.0 14,245 - -0.3 14,260 - -0.3 14,264 + +0.2 14,264 + -2.0 14,325 + dnaJ b0015 0.14 -0.9 14,371 + dnaJ b0015 0.18 -1.6 14,374 - dnaJ b0015 0.18 -0.9 14,508 + dnaJ b0015 0.30 -2.3 14,508 + dnaJ b0015 0.30 -1.1 14,521 + dnaJ b0015 0.31 -0.9 14,594 - dnaJ b0015 0.38 -1.0 14,594 - dnaJ b0015 0.38 -0.9 14,594 - dnaJ b0015 0.38 -0.6 14,596 + dnaJ b0015 0.38 -0.5 14,596 + dnaJ b0015 0.38 -1.3 14,596 - dnaJ b0015 0.38 -0.1 14,627 - dnaJ b0015 0.41 -0.9 14,664 + dnaJ b0015 0.44 -0.5 14,664 + dnaJ b0015 0.44 +0.1 14,672 - dnaJ b0015 0.45 -1.1 14,682 - dnaJ b0015 0.45 -0.1 14,702 + dnaJ b0015 0.47 -0.4 14,713 - dnaJ b0015 0.48 -3.2 14,713 - dnaJ b0015 0.48 -3.0 14,713 - dnaJ b0015 0.48 -0.4 14,745 + dnaJ b0015 0.51 -1.1 14,781 - dnaJ b0015 0.54 -0.9 14,781 - dnaJ b0015 0.54 -0.9 14,829 + dnaJ b0015 0.58 -1.5 14,829 + dnaJ b0015 0.58 -2.3 14,842 - dnaJ b0015 0.60 -0.8 14,842 - dnaJ b0015 0.60 +0.1 14,859 + dnaJ b0015 0.61 -1.3 14,859 + dnaJ b0015 0.61 -1.6 14,923 + dnaJ b0015 0.67 +0.0 14,959 - dnaJ b0015 0.70 -0.2 15,087 + dnaJ b0015 0.81 -0.6 15,087 + dnaJ b0015 0.81 -2.3 15,111 + dnaJ b0015 0.83 -2.7 15,111 + dnaJ b0015 0.83 -0.4 15,111 + dnaJ b0015 0.83 -1.2 15,143 + dnaJ b0015 0.86 -1.4 15,164 - dnaJ b0015 0.88 +0.1 15,235 - +0.5 15,235 - +0.4 15,289 + -1.0 15,389 - +0.0 15,410 - -0.1 15,439 - +0.7
Or see this region's nucleotide sequence