Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides thrC and yaaX are separated by 213 nucleotides yaaX and yaaA are separated by 152 nucleotides
b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799
thrA
b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733
thrB
b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020
thrC
b0005: yaaX - hypothetical protein (NCBI), at 5,234 to 5,530
yaaX
b0006: yaaA - hypothetical protein (NCBI), at 5,683 to 6,459
yaaA
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 2.744 kb on - strand at 2.787 kb on - strand at 2.787 kb on - strand at 2.829 kb on + strand at 2.847 kb on + strand at 2.847 kb on + strand at 2.906 kb on - strand, within thrB at 2.934 kb on + strand, within thrB at 2.963 kb on - strand, within thrB at 3.020 kb on - strand, within thrB at 3.023 kb on + strand, within thrB at 3.131 kb on + strand, within thrB at 3.144 kb on - strand, within thrB at 3.186 kb on + strand, within thrB at 3.246 kb on + strand, within thrB at 3.248 kb on + strand, within thrB at 3.256 kb on + strand, within thrB at 3.293 kb on + strand, within thrB at 3.301 kb on - strand, within thrB at 3.318 kb on - strand, within thrB at 3.411 kb on + strand, within thrB at 3.444 kb on - strand, within thrB at 3.444 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.509 kb on - strand, within thrB at 3.511 kb on + strand, within thrB at 3.607 kb on + strand, within thrB at 3.634 kb on - strand, within thrB at 3.638 kb on + strand, within thrB at 3.638 kb on + strand, within thrB at 3.650 kb on + strand at 3.769 kb on + strand at 3.769 kb on + strand at 3.792 kb on - strand at 3.805 kb on - strand at 3.953 kb on - strand, within thrC at 3.953 kb on - strand, within thrC at 4.108 kb on - strand, within thrC at 4.174 kb on - strand, within thrC at 4.180 kb on + strand, within thrC at 4.180 kb on + strand, within thrC at 4.216 kb on + strand, within thrC at 4.253 kb on - strand, within thrC at 4.253 kb on - strand, within thrC at 4.464 kb on - strand, within thrC at 4.466 kb on + strand, within thrC at 4.619 kb on + strand, within thrC at 4.721 kb on + strand, within thrC at 4.729 kb on + strand, within thrC at 4.729 kb on - strand, within thrC at 4.729 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.771 kb on + strand, within thrC at 4.771 kb on + strand, within thrC at 4.779 kb on - strand, within thrC at 4.779 kb on - strand, within thrC at 4.796 kb on + strand, within thrC at 4.875 kb on + strand, within thrC at 4.932 kb on + strand at 4.932 kb on + strand at 4.993 kb on + strand at 5.238 kb on + strand at 5.241 kb on + strand at 5.241 kb on + strand at 5.273 kb on + strand, within yaaX at 5.273 kb on + strand, within yaaX at 5.280 kb on - strand, within yaaX at 5.283 kb on + strand, within yaaX at 5.285 kb on + strand, within yaaX at 5.358 kb on - strand, within yaaX at 5.470 kb on + strand, within yaaX at 5.470 kb on + strand, within yaaX at 5.639 kb on - strand at 5.699 kb on - strand at 5.699 kb on - strand at 5.788 kb on - strand, within yaaA at 5.788 kb on - strand, within yaaA at 5.835 kb on - strand, within yaaA at 5.879 kb on - strand, within yaaA at 5.891 kb on - strand, within yaaA at 5.898 kb on + strand, within yaaA at 5.958 kb on + strand, within yaaA at 5.976 kb on + strand, within yaaA at 5.976 kb on + strand, within yaaA at 5.995 kb on + strand, within yaaA at 6.005 kb on - strand, within yaaA
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 2,744 - -2.5 2,787 - -3.3 2,787 - -2.5 2,829 + -3.3 2,847 + -2.6 2,847 + -2.4 2,906 - thrB b0003 0.11 -2.6 2,934 + thrB b0003 0.14 -2.5 2,963 - thrB b0003 0.17 -1.8 3,020 - thrB b0003 0.23 -0.5 3,023 + thrB b0003 0.24 -1.6 3,131 + thrB b0003 0.35 -2.4 3,144 - thrB b0003 0.37 -2.9 3,186 + thrB b0003 0.41 -1.7 3,246 + thrB b0003 0.48 -2.3 3,248 + thrB b0003 0.48 -3.0 3,256 + thrB b0003 0.49 -2.3 3,293 + thrB b0003 0.53 -2.7 3,301 - thrB b0003 0.54 -3.4 3,318 - thrB b0003 0.55 -2.2 3,411 + thrB b0003 0.65 -4.1 3,444 - thrB b0003 0.69 -4.1 3,444 - thrB b0003 0.69 -0.7 3,504 - thrB b0003 0.75 -2.9 3,504 - thrB b0003 0.75 -2.5 3,509 - thrB b0003 0.76 -3.4 3,511 + thrB b0003 0.76 -3.5 3,607 + thrB b0003 0.86 -3.8 3,634 - thrB b0003 0.89 -3.0 3,638 + thrB b0003 0.90 -1.9 3,638 + thrB b0003 0.90 -2.9 3,650 + -2.0 3,769 + -2.8 3,769 + -3.6 3,792 - -4.1 3,805 - -3.1 3,953 - thrC b0004 0.17 -3.5 3,953 - thrC b0004 0.17 -2.4 4,108 - thrC b0004 0.29 -3.1 4,174 - thrC b0004 0.34 -2.4 4,180 + thrC b0004 0.35 -2.1 4,180 + thrC b0004 0.35 -3.3 4,216 + thrC b0004 0.37 -2.9 4,253 - thrC b0004 0.40 -1.8 4,253 - thrC b0004 0.40 -2.2 4,464 - thrC b0004 0.57 -3.1 4,466 + thrC b0004 0.57 -4.1 4,619 + thrC b0004 0.69 -4.0 4,721 + thrC b0004 0.77 -2.3 4,729 + thrC b0004 0.77 -3.0 4,729 - thrC b0004 0.77 -3.9 4,729 - thrC b0004 0.77 -3.4 4,755 - thrC b0004 0.79 -2.6 4,755 - thrC b0004 0.79 -2.1 4,755 - thrC b0004 0.79 -2.5 4,771 + thrC b0004 0.81 -2.3 4,771 + thrC b0004 0.81 -2.5 4,779 - thrC b0004 0.81 -2.1 4,779 - thrC b0004 0.81 -4.8 4,796 + thrC b0004 0.83 -2.6 4,875 + thrC b0004 0.89 -1.9 4,932 + -2.0 4,932 + -2.0 4,993 + +0.4 5,238 + +0.1 5,241 + +1.3 5,241 + -0.8 5,273 + yaaX b0005 0.13 -0.5 5,273 + yaaX b0005 0.13 +0.6 5,280 - yaaX b0005 0.15 -0.3 5,283 + yaaX b0005 0.16 +0.1 5,285 + yaaX b0005 0.17 +0.2 5,358 - yaaX b0005 0.42 +0.3 5,470 + yaaX b0005 0.79 -2.4 5,470 + yaaX b0005 0.79 +0.2 5,639 - +0.2 5,699 - -0.7 5,699 - -0.1 5,788 - yaaA b0006 0.14 +0.9 5,788 - yaaA b0006 0.14 +0.3 5,835 - yaaA b0006 0.20 +0.1 5,879 - yaaA b0006 0.25 -1.0 5,891 - yaaA b0006 0.27 +0.1 5,898 + yaaA b0006 0.28 +0.3 5,958 + yaaA b0006 0.35 -0.2 5,976 + yaaA b0006 0.38 +0.6 5,976 + yaaA b0006 0.38 -0.1 5,995 + yaaA b0006 0.40 -0.6 6,005 - yaaA b0006 0.41 -1.3
Or see this region's nucleotide sequence