Strain Fitness in Escherichia coli BW25113 around b0883

Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclpA and serW are separated by 343 nucleotidesserW and infA are separated by 253 nucleotidesinfA and aat are separated by 284 nucleotides b0882: clpA - ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI), at 922,487 to 924,763 clpA b0883: serW - tRNA-Ser (NCBI), at 925,107 to 925,194 serW b0884: infA - translation initiation factor IF-1 (NCBI), at 925,448 to 925,666 infA b0885: aat - leucyl/phenylalanyl-tRNA--protein transferase (NCBI), at 925,951 to 926,655 aat Position (kb) 925 926Strain fitness (log2 ratio) -2 -1 0 1at 924.151 kb on - strand, within clpAat 924.151 kb on - strand, within clpAat 924.151 kb on - strand, within clpAat 924.344 kb on + strand, within clpAat 924.344 kb on + strand, within clpAat 924.542 kb on + strandat 924.566 kb on + strandat 924.616 kb on - strandat 924.616 kb on - strandat 924.644 kb on + strandat 924.644 kb on + strandat 924.734 kb on + strandat 924.734 kb on + strandat 924.765 kb on - strandat 924.780 kb on + strandat 924.954 kb on + strandat 925.246 kb on - strandat 925.246 kb on - strandat 925.319 kb on + strandat 925.319 kb on + strandat 925.319 kb on + strandat 925.327 kb on - strandat 925.686 kb on - strandat 925.687 kb on - strandat 925.687 kb on - strandat 925.779 kb on - strandat 925.885 kb on + strandat 925.967 kb on + strandat 925.967 kb on + strandat 926.089 kb on + strand, within aat

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
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924,151 - clpA b0882 0.73 -0.1
924,151 - clpA b0882 0.73 +1.0
924,151 - clpA b0882 0.73 -0.2
924,344 + clpA b0882 0.82 -0.2
924,344 + clpA b0882 0.82 -0.1
924,542 + -1.1
924,566 + -1.8
924,616 - -0.3
924,616 - -0.6
924,644 + -0.3
924,644 + +0.5
924,734 + +1.2
924,734 + -0.7
924,765 - +0.6
924,780 + +1.0
924,954 + +0.6
925,246 - +0.0
925,246 - -0.6
925,319 + -0.2
925,319 + -0.4
925,319 + -0.6
925,327 - +0.1
925,686 - +0.1
925,687 - -0.1
925,687 - +0.7
925,779 - -0.2
925,885 + +0.3
925,967 + +0.5
925,967 + +0.3
926,089 + aat b0885 0.20 -0.6

Or see this region's nucleotide sequence