Strain Fitness in Escherichia coli BW25113 around b2424

Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
2,538,824	+				-3.1
2,538,824	+				-0.7
2,538,846	-				+0.6
2,538,846	-				+0.3
2,538,952	+	cysW	b2423	0.14	-1.0
2,538,952	+	cysW	b2423	0.14	+0.2
2,538,959	+	cysW	b2423	0.15	-0.6
2,538,961	+	cysW	b2423	0.15	-2.4
2,538,986	+	cysW	b2423	0.18	-1.3
2,539,072	+	cysW	b2423	0.28	-0.6
2,539,173	+	cysW	b2423	0.40	-1.3
2,539,173	+	cysW	b2423	0.40	-1.5
2,539,240	+	cysW	b2423	0.47	-0.8
2,539,249	-	cysW	b2423	0.48	-1.4
2,539,249	-	cysW	b2423	0.48	-2.2
2,539,456	+	cysW	b2423	0.72	-1.1
2,539,468	-	cysW	b2423	0.73	+0.3
2,539,468	-	cysW	b2423	0.73	+1.1
2,539,468	-	cysW	b2423	0.73	-1.2
2,539,694	-				-0.8
2,540,062	+	cysU	b2424	0.43	-1.3
2,540,110	-	cysU	b2424	0.49	-1.2
2,540,110	-	cysU	b2424	0.49	-0.9
2,540,129	+	cysU	b2424	0.51	-3.4
2,540,492	+				-2.4
2,540,535	+				-1.6
2,540,563	-				-0.9
2,540,677	-	cysP	b2425	0.14	+0.0
2,540,677	-	cysP	b2425	0.14	+1.0
2,540,695	-	cysP	b2425	0.16	-0.9
2,540,752	-	cysP	b2425	0.21	-1.3
2,540,816	-	cysP	b2425	0.28	+0.2
2,540,858	+	cysP	b2425	0.32	-1.3
2,540,872	+	cysP	b2425	0.33	-1.9
2,541,064	+	cysP	b2425	0.52	-1.2
2,541,066	+	cysP	b2425	0.52	-0.1
2,541,077	-	cysP	b2425	0.53	+0.5
2,541,159	+	cysP	b2425	0.61	-1.2
2,541,181	-	cysP	b2425	0.64	-0.1
2,541,194	+	cysP	b2425	0.65	-0.9
2,541,229	-	cysP	b2425	0.68	+1.1
2,541,421	-	cysP	b2425	0.87	-0.8