Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cydB and ybgT are separated by 14 nucleotides ybgT and ybgE overlap by 1 nucleotides ybgE and ybgC are separated by 149 nucleotides ybgC and tolQ overlap by 4 nucleotides tolQ and tolR are separated by 3 nucleotides tolR and tolA are separated by 64 nucleotides
b0734: cydB - cytochrome d terminal oxidase, subunit II (NCBI), at 772,265 to 773,404
cydB
b4515: ybgT - hypothetical protein (NCBI), at 773,419 to 773,532
ybgT
b0735: ybgE - conserved inner membrane protein (NCBI), at 773,532 to 773,825
ybgE
b0736: ybgC - predicted acyl-CoA thioesterase (NCBI), at 773,975 to 774,379
ybgC
b0737: tolQ - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 774,376 to 775,068
tolQ
b0738: tolR - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 775,072 to 775,500
tolR
b0739: tolA - cell envelope integrity inner membrane protein TolA (NCBI), at 775,565 to 776,830
tolA
Position (kb) 774
775
776 Strain fitness (log2 ratio) -2
-1
0
1
2 at 773.380 kb on + strand at 773.486 kb on + strand, within ybgT at 773.572 kb on + strand, within ybgE at 773.617 kb on - strand, within ybgE at 773.693 kb on + strand, within ybgE at 773.693 kb on + strand, within ybgE at 773.702 kb on + strand, within ybgE at 773.702 kb on + strand, within ybgE at 773.857 kb on + strand at 773.857 kb on + strand at 773.900 kb on - strand at 773.900 kb on - strand at 773.968 kb on + strand at 773.974 kb on + strand at 773.974 kb on + strand at 774.007 kb on + strand at 774.007 kb on + strand at 774.103 kb on + strand, within ybgC at 774.103 kb on + strand, within ybgC at 774.256 kb on + strand, within ybgC at 774.265 kb on - strand, within ybgC at 774.299 kb on - strand, within ybgC at 774.336 kb on + strand, within ybgC at 774.390 kb on - strand at 774.561 kb on + strand, within tolQ at 774.571 kb on + strand, within tolQ at 774.649 kb on + strand, within tolQ at 774.668 kb on - strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.809 kb on - strand, within tolQ at 774.813 kb on - strand, within tolQ at 774.818 kb on - strand, within tolQ at 774.839 kb on + strand, within tolQ at 774.905 kb on + strand, within tolQ at 775.063 kb on + strand at 775.063 kb on + strand at 775.070 kb on - strand at 775.071 kb on - strand at 775.071 kb on - strand at 775.091 kb on - strand at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.146 kb on + strand, within tolR at 775.152 kb on - strand, within tolR at 775.194 kb on - strand, within tolR at 775.258 kb on + strand, within tolR at 775.265 kb on + strand, within tolR at 775.275 kb on + strand, within tolR at 775.280 kb on + strand, within tolR at 775.288 kb on - strand, within tolR at 775.288 kb on - strand, within tolR at 775.298 kb on - strand, within tolR at 775.333 kb on + strand, within tolR at 775.333 kb on + strand, within tolR at 775.357 kb on - strand, within tolR at 775.526 kb on - strand at 775.533 kb on + strand at 775.565 kb on + strand at 775.628 kb on + strand at 775.651 kb on + strand at 775.659 kb on - strand at 775.672 kb on + strand at 775.748 kb on + strand, within tolA at 775.753 kb on + strand, within tolA at 775.840 kb on + strand, within tolA at 775.930 kb on - strand, within tolA at 775.932 kb on + strand, within tolA at 775.947 kb on + strand, within tolA
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 25 uM remove 773,380 + -1.3 773,486 + ybgT b4515 0.59 -1.1 773,572 + ybgE b0735 0.14 -0.1 773,617 - ybgE b0735 0.29 -0.4 773,693 + ybgE b0735 0.55 -1.1 773,693 + ybgE b0735 0.55 +0.2 773,702 + ybgE b0735 0.58 +0.2 773,702 + ybgE b0735 0.58 +0.2 773,857 + +0.2 773,857 + +0.4 773,900 - +0.3 773,900 - -0.1 773,968 + -0.3 773,974 + +1.3 773,974 + +1.0 774,007 + -0.1 774,007 + -0.1 774,103 + ybgC b0736 0.32 -0.1 774,103 + ybgC b0736 0.32 -0.2 774,256 + ybgC b0736 0.69 -0.1 774,265 - ybgC b0736 0.72 +0.7 774,299 - ybgC b0736 0.80 +0.6 774,336 + ybgC b0736 0.89 +0.4 774,390 - +1.0 774,561 + tolQ b0737 0.27 -1.3 774,571 + tolQ b0737 0.28 -1.3 774,649 + tolQ b0737 0.39 +0.3 774,668 - tolQ b0737 0.42 +0.4 774,723 + tolQ b0737 0.50 -1.4 774,723 + tolQ b0737 0.50 -1.1 774,809 - tolQ b0737 0.62 -0.9 774,813 - tolQ b0737 0.63 -1.9 774,818 - tolQ b0737 0.64 -0.8 774,839 + tolQ b0737 0.67 -0.6 774,905 + tolQ b0737 0.76 -1.8 775,063 + +0.5 775,063 + -0.1 775,070 - -0.5 775,071 - -0.2 775,071 - +0.1 775,091 - +0.1 775,121 + tolR b0738 0.11 +0.4 775,121 + tolR b0738 0.11 +1.2 775,121 + tolR b0738 0.11 +0.6 775,146 + tolR b0738 0.17 +0.4 775,152 - tolR b0738 0.19 +0.6 775,194 - tolR b0738 0.28 -0.6 775,258 + tolR b0738 0.43 +1.0 775,265 + tolR b0738 0.45 -0.3 775,275 + tolR b0738 0.47 +2.0 775,280 + tolR b0738 0.48 -0.3 775,288 - tolR b0738 0.50 -0.3 775,288 - tolR b0738 0.50 +2.0 775,298 - tolR b0738 0.53 -1.3 775,333 + tolR b0738 0.61 -0.6 775,333 + tolR b0738 0.61 -1.0 775,357 - tolR b0738 0.66 +0.0 775,526 - +1.8 775,533 + -0.2 775,565 + -1.0 775,628 + +0.0 775,651 + -0.9 775,659 - +1.0 775,672 + +0.2 775,748 + tolA b0739 0.14 -0.4 775,753 + tolA b0739 0.15 +1.0 775,840 + tolA b0739 0.22 +0.0 775,930 - tolA b0739 0.29 -1.1 775,932 + tolA b0739 0.29 +1.0 775,947 + tolA b0739 0.30 -1.4
Or see this region's nucleotide sequence