Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 0.25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahC and yahD are separated by 257 nucleotides yahD and yahE are separated by 39 nucleotides yahE and yahF overlap by 11 nucleotides
b0317: yahC - predicted inner membrane protein (NCBI), at 333,749 to 334,246
yahC
b0318: yahD - predicted transcriptional regulator with ankyrin domain (NCBI), at 334,504 to 335,109
yahD
b0319: yahE - hypothetical protein (NCBI), at 335,149 to 336,012
yahE
b0320: yahF - predicted acyl-CoA synthetase with NAD(P)-binding domain and succinyl-CoA synthetase domain (NCBI), at 336,002 to 337,549
yahF
Position (kb) 335
336
337 Strain fitness (log2 ratio) -2
-1
0
1
2 at 334.186 kb on - strand, within yahC at 334.238 kb on - strand at 334.240 kb on + strand at 334.260 kb on + strand at 334.260 kb on + strand at 334.260 kb on + strand at 334.260 kb on + strand at 334.260 kb on + strand at 334.281 kb on + strand at 334.282 kb on + strand at 334.282 kb on + strand at 334.306 kb on - strand at 334.323 kb on + strand at 334.323 kb on + strand at 334.330 kb on + strand at 334.330 kb on + strand at 334.361 kb on + strand at 334.361 kb on + strand at 334.369 kb on - strand at 334.459 kb on + strand at 334.459 kb on + strand at 334.497 kb on + strand at 334.532 kb on + strand at 334.532 kb on + strand at 334.541 kb on + strand at 334.543 kb on + strand at 334.543 kb on + strand at 334.548 kb on + strand at 334.548 kb on + strand at 334.567 kb on + strand, within yahD at 334.782 kb on + strand, within yahD at 334.790 kb on - strand, within yahD at 334.791 kb on - strand, within yahD at 334.791 kb on - strand, within yahD at 334.805 kb on - strand, within yahD at 334.805 kb on - strand, within yahD at 334.809 kb on - strand, within yahD at 334.826 kb on + strand, within yahD at 334.828 kb on - strand, within yahD at 334.828 kb on - strand, within yahD at 334.835 kb on - strand, within yahD at 334.839 kb on - strand, within yahD at 334.895 kb on + strand, within yahD at 334.903 kb on - strand, within yahD at 334.903 kb on - strand, within yahD at 334.903 kb on - strand, within yahD at 334.908 kb on + strand, within yahD at 334.908 kb on + strand, within yahD at 334.908 kb on - strand, within yahD at 334.911 kb on + strand at 334.911 kb on + strand, within yahD at 334.927 kb on - strand, within yahD at 334.969 kb on - strand, within yahD at 334.969 kb on - strand, within yahD at 334.986 kb on + strand, within yahD at 334.998 kb on + strand, within yahD at 335.009 kb on + strand, within yahD at 335.009 kb on + strand, within yahD at 335.009 kb on + strand, within yahD at 335.009 kb on + strand, within yahD at 335.017 kb on - strand, within yahD at 335.017 kb on - strand, within yahD at 335.058 kb on + strand at 335.058 kb on + strand at 335.088 kb on + strand at 335.089 kb on - strand at 335.118 kb on - strand at 335.122 kb on + strand at 335.122 kb on + strand at 335.181 kb on + strand at 335.214 kb on + strand at 335.214 kb on + strand at 335.278 kb on - strand, within yahE at 335.280 kb on + strand, within yahE at 335.280 kb on + strand, within yahE at 335.280 kb on + strand, within yahE at 335.280 kb on + strand, within yahE at 335.286 kb on - strand, within yahE at 335.324 kb on + strand, within yahE at 335.324 kb on - strand, within yahE at 335.343 kb on + strand, within yahE at 335.357 kb on + strand, within yahE at 335.372 kb on + strand, within yahE at 335.372 kb on + strand, within yahE at 335.391 kb on + strand, within yahE at 335.391 kb on + strand, within yahE at 335.396 kb on + strand, within yahE at 335.417 kb on + strand, within yahE at 335.417 kb on + strand, within yahE at 335.418 kb on + strand, within yahE at 335.418 kb on + strand, within yahE at 335.429 kb on + strand, within yahE at 335.429 kb on + strand, within yahE at 335.469 kb on + strand, within yahE at 335.473 kb on + strand, within yahE at 335.473 kb on + strand, within yahE at 335.474 kb on + strand, within yahE at 335.479 kb on + strand, within yahE at 335.482 kb on + strand, within yahE at 335.494 kb on + strand, within yahE at 335.516 kb on + strand, within yahE at 335.652 kb on + strand, within yahE at 335.653 kb on + strand, within yahE at 335.654 kb on + strand, within yahE at 335.654 kb on + strand, within yahE at 335.654 kb on + strand, within yahE at 335.655 kb on + strand, within yahE at 335.655 kb on + strand, within yahE at 335.657 kb on - strand, within yahE at 335.661 kb on - strand, within yahE at 335.666 kb on + strand, within yahE at 335.673 kb on + strand, within yahE at 335.817 kb on - strand, within yahE at 335.817 kb on - strand, within yahE at 335.883 kb on + strand, within yahE at 335.883 kb on + strand, within yahE at 335.899 kb on - strand, within yahE at 335.899 kb on - strand, within yahE at 335.904 kb on - strand, within yahE at 335.944 kb on + strand at 335.944 kb on + strand at 335.949 kb on + strand at 335.950 kb on + strand at 335.950 kb on + strand at 335.950 kb on + strand at 335.952 kb on + strand at 335.952 kb on + strand at 335.957 kb on - strand at 335.957 kb on - strand at 335.958 kb on - strand at 335.958 kb on - strand at 335.958 kb on - strand at 335.960 kb on - strand at 335.960 kb on - strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.974 kb on + strand at 335.974 kb on + strand at 335.974 kb on - strand at 335.977 kb on - strand at 335.977 kb on - strand at 335.977 kb on - strand at 335.986 kb on + strand at 335.988 kb on - strand at 335.995 kb on - strand at 335.995 kb on - strand at 336.005 kb on - strand at 336.005 kb on - strand at 336.084 kb on - strand at 336.218 kb on - strand, within yahF at 336.218 kb on - strand, within yahF at 336.227 kb on + strand, within yahF at 336.236 kb on + strand, within yahF at 336.238 kb on + strand, within yahF at 336.252 kb on + strand, within yahF at 336.262 kb on + strand, within yahF at 336.262 kb on + strand, within yahF at 336.294 kb on + strand, within yahF at 336.306 kb on - strand, within yahF at 336.306 kb on - strand, within yahF at 336.308 kb on + strand, within yahF at 336.368 kb on + strand, within yahF at 336.368 kb on + strand, within yahF at 336.554 kb on - strand, within yahF
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 0.25 uM remove 334,186 - yahC b0317 0.88 +0.8 334,238 - -0.4 334,240 + +0.0 334,260 + -0.9 334,260 + +0.2 334,260 + +0.7 334,260 + -0.2 334,260 + +1.9 334,281 + +0.0 334,282 + +0.2 334,282 + +0.1 334,306 - -0.1 334,323 + +0.1 334,323 + +0.1 334,330 + +0.1 334,330 + -0.8 334,361 + -0.4 334,361 + -0.0 334,369 - -1.0 334,459 + +1.6 334,459 + +0.1 334,497 + +0.8 334,532 + +0.5 334,532 + -1.4 334,541 + -0.1 334,543 + +0.7 334,543 + +0.9 334,548 + -1.1 334,548 + +0.2 334,567 + yahD b0318 0.10 -0.2 334,782 + yahD b0318 0.46 +1.9 334,790 - yahD b0318 0.47 +0.7 334,791 - yahD b0318 0.47 -0.3 334,791 - yahD b0318 0.47 -0.7 334,805 - yahD b0318 0.50 -1.0 334,805 - yahD b0318 0.50 +0.2 334,809 - yahD b0318 0.50 -0.2 334,826 + yahD b0318 0.53 +1.4 334,828 - yahD b0318 0.53 +1.9 334,828 - yahD b0318 0.53 -0.1 334,835 - yahD b0318 0.55 -0.4 334,839 - yahD b0318 0.55 +0.4 334,895 + yahD b0318 0.65 -1.2 334,903 - yahD b0318 0.66 -0.9 334,903 - yahD b0318 0.66 -1.3 334,903 - yahD b0318 0.66 +1.2 334,908 + yahD b0318 0.67 -0.9 334,908 + yahD b0318 0.67 -0.2 334,908 - yahD b0318 0.67 -1.4 334,911 + +0.4 334,911 + yahD b0318 0.67 -0.9 334,927 - yahD b0318 0.70 -1.0 334,969 - yahD b0318 0.77 -0.0 334,969 - yahD b0318 0.77 +0.0 334,986 + yahD b0318 0.80 +0.1 334,998 + yahD b0318 0.82 -0.6 335,009 + yahD b0318 0.83 -0.6 335,009 + yahD b0318 0.83 +0.0 335,009 + yahD b0318 0.83 +0.4 335,009 + yahD b0318 0.83 -0.5 335,017 - yahD b0318 0.85 +0.7 335,017 - yahD b0318 0.85 +0.4 335,058 + +0.3 335,058 + +0.5 335,088 + -0.5 335,089 - -1.4 335,118 - +0.3 335,122 + +0.7 335,122 + -0.9 335,181 + -0.2 335,214 + +0.4 335,214 + +0.3 335,278 - yahE b0319 0.15 +1.7 335,280 + yahE b0319 0.15 +0.1 335,280 + yahE b0319 0.15 +0.9 335,280 + yahE b0319 0.15 +0.1 335,280 + yahE b0319 0.15 +0.5 335,286 - yahE b0319 0.16 -0.5 335,324 + yahE b0319 0.20 -0.2 335,324 - yahE b0319 0.20 -0.2 335,343 + yahE b0319 0.22 -1.0 335,357 + yahE b0319 0.24 -0.1 335,372 + yahE b0319 0.26 -0.2 335,372 + yahE b0319 0.26 +0.2 335,391 + yahE b0319 0.28 +0.5 335,391 + yahE b0319 0.28 +0.3 335,396 + yahE b0319 0.29 +0.2 335,417 + yahE b0319 0.31 +0.1 335,417 + yahE b0319 0.31 +0.2 335,418 + yahE b0319 0.31 -0.1 335,418 + yahE b0319 0.31 -0.3 335,429 + yahE b0319 0.32 +1.6 335,429 + yahE b0319 0.32 +0.5 335,469 + yahE b0319 0.37 -1.9 335,473 + yahE b0319 0.38 -0.5 335,473 + yahE b0319 0.38 -0.2 335,474 + yahE b0319 0.38 +0.4 335,479 + yahE b0319 0.38 -0.2 335,482 + yahE b0319 0.39 -0.2 335,494 + yahE b0319 0.40 -0.1 335,516 + yahE b0319 0.42 +0.0 335,652 + yahE b0319 0.58 +1.9 335,653 + yahE b0319 0.58 +1.9 335,654 + yahE b0319 0.58 +0.9 335,654 + yahE b0319 0.58 +1.9 335,654 + yahE b0319 0.58 +1.9 335,655 + yahE b0319 0.59 -0.4 335,655 + yahE b0319 0.59 +1.9 335,657 - yahE b0319 0.59 +1.9 335,661 - yahE b0319 0.59 +1.9 335,666 + yahE b0319 0.60 +1.9 335,673 + yahE b0319 0.61 -0.2 335,817 - yahE b0319 0.77 +1.9 335,817 - yahE b0319 0.77 +1.9 335,883 + yahE b0319 0.85 +0.9 335,883 + yahE b0319 0.85 +0.4 335,899 - yahE b0319 0.87 -0.6 335,899 - yahE b0319 0.87 -1.5 335,904 - yahE b0319 0.87 +1.5 335,944 + -0.1 335,944 + +0.9 335,949 + +1.3 335,950 + +0.0 335,950 + +0.2 335,950 + +0.1 335,952 + -0.1 335,952 + +0.9 335,957 - -1.6 335,957 - +0.3 335,958 - -0.0 335,958 - -0.5 335,958 - -0.2 335,960 - +0.6 335,960 - +0.4 335,969 + -1.1 335,969 + +0.2 335,969 + -0.8 335,969 + +1.1 335,969 + +0.7 335,969 + -0.3 335,969 + +0.4 335,969 + +0.3 335,970 - +0.1 335,970 - +0.0 335,970 - -0.8 335,970 - +0.2 335,970 - +1.4 335,970 - +0.6 335,974 + +1.0 335,974 + -1.7 335,974 - -0.9 335,977 - +0.1 335,977 - -0.8 335,977 - -1.9 335,986 + -0.4 335,988 - +1.9 335,995 - +0.2 335,995 - +0.5 336,005 - -0.1 336,005 - -0.6 336,084 - +0.1 336,218 - yahF b0320 0.14 +1.9 336,218 - yahF b0320 0.14 +0.8 336,227 + yahF b0320 0.15 +0.1 336,236 + yahF b0320 0.15 +0.4 336,238 + yahF b0320 0.15 +0.2 336,252 + yahF b0320 0.16 +0.1 336,262 + yahF b0320 0.17 +0.1 336,262 + yahF b0320 0.17 +0.2 336,294 + yahF b0320 0.19 -0.1 336,306 - yahF b0320 0.20 +0.1 336,306 - yahF b0320 0.20 +0.0 336,308 + yahF b0320 0.20 -1.8 336,368 + yahF b0320 0.24 -0.1 336,368 + yahF b0320 0.24 -0.4 336,554 - yahF b0320 0.36 +0.2
Or see this region's nucleotide sequence