Experiment: M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 0.25 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeB and rcsF overlap by 4 nucleotides rcsF and metQ are separated by 117 nucleotides metQ and metI are separated by 39 nucleotides metI and metN overlap by 8 nucleotides
b0195: yaeB - hypothetical protein (NCBI), at 218,887 to 219,594
yaeB
b0196: rcsF - predicted outer membrane protein, signal (NCBI), at 219,591 to 219,995
rcsF
b0197: metQ - DL-methionine transporter subunit (NCBI), at 220,113 to 220,928
metQ
b0198: metI - DL-methionine transporter subunit (NCBI), at 220,968 to 221,621
metI
b0199: metN - DL-methionine transporter subunit (NCBI), at 221,614 to 222,645
metN
Position (kb) 220
221 Strain fitness (log2 ratio) -2
-1
0
1 at 219.200 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.261 kb on + strand, within yaeB at 219.312 kb on + strand, within yaeB at 219.446 kb on - strand, within yaeB at 219.465 kb on + strand, within yaeB at 219.466 kb on + strand, within yaeB at 219.489 kb on - strand, within yaeB at 219.513 kb on + strand, within yaeB at 219.548 kb on - strand at 219.598 kb on + strand at 219.598 kb on + strand at 219.643 kb on - strand, within rcsF at 219.678 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.756 kb on + strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.842 kb on - strand, within rcsF at 219.902 kb on + strand, within rcsF at 219.944 kb on - strand, within rcsF at 219.968 kb on + strand at 219.976 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.127 kb on - strand at 220.127 kb on - strand at 220.249 kb on - strand, within metQ at 220.321 kb on + strand, within metQ at 220.321 kb on + strand, within metQ at 220.323 kb on + strand, within metQ at 220.361 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.405 kb on + strand, within metQ at 220.405 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.504 kb on + strand, within metQ at 220.532 kb on + strand, within metQ at 220.543 kb on + strand, within metQ at 220.661 kb on + strand, within metQ at 220.675 kb on + strand, within metQ at 220.694 kb on - strand, within metQ at 220.694 kb on - strand, within metQ at 220.750 kb on - strand, within metQ at 220.754 kb on + strand, within metQ at 220.764 kb on + strand, within metQ at 220.825 kb on + strand, within metQ at 220.865 kb on + strand at 221.043 kb on - strand, within metI at 221.047 kb on + strand, within metI at 221.052 kb on + strand, within metI at 221.060 kb on - strand, within metI at 221.076 kb on + strand, within metI at 221.076 kb on + strand, within metI at 221.080 kb on + strand, within metI at 221.080 kb on + strand, within metI at 221.096 kb on - strand, within metI at 221.105 kb on - strand, within metI at 221.106 kb on - strand, within metI at 221.128 kb on - strand, within metI at 221.148 kb on + strand, within metI at 221.148 kb on + strand, within metI at 221.181 kb on + strand, within metI at 221.214 kb on + strand, within metI at 221.238 kb on + strand, within metI at 221.238 kb on + strand, within metI at 221.250 kb on - strand, within metI at 221.269 kb on - strand, within metI at 221.280 kb on + strand, within metI at 221.300 kb on - strand, within metI at 221.347 kb on - strand, within metI at 221.383 kb on - strand, within metI at 221.383 kb on - strand, within metI at 221.466 kb on - strand, within metI at 221.469 kb on - strand, within metI at 221.511 kb on - strand, within metI at 221.511 kb on - strand, within metI at 221.595 kb on - strand at 221.595 kb on - strand at 221.619 kb on + strand at 221.623 kb on + strand at 221.655 kb on - strand at 221.680 kb on + strand at 221.685 kb on + strand at 221.688 kb on - strand at 221.802 kb on + strand, within metN at 221.830 kb on + strand, within metN at 221.830 kb on + strand, within metN at 221.846 kb on + strand, within metN at 221.847 kb on + strand, within metN at 221.848 kb on + strand, within metN at 221.856 kb on + strand, within metN at 221.911 kb on - strand, within metN
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Pyridoxine HCl 100 nM and 4-Deoxypyridoxine 0.25 uM remove 219,200 + yaeB b0195 0.44 +0.8 219,240 + yaeB b0195 0.50 -0.2 219,240 + yaeB b0195 0.50 -0.1 219,261 + yaeB b0195 0.53 +1.1 219,312 + yaeB b0195 0.60 +0.3 219,446 - yaeB b0195 0.79 +0.2 219,465 + yaeB b0195 0.82 +0.6 219,466 + yaeB b0195 0.82 +0.9 219,489 - yaeB b0195 0.85 +0.1 219,513 + yaeB b0195 0.88 +0.3 219,548 - +0.1 219,598 + +0.9 219,598 + +1.6 219,643 - rcsF b0196 0.13 +0.0 219,678 + rcsF b0196 0.21 +0.8 219,680 + rcsF b0196 0.22 +0.4 219,680 + rcsF b0196 0.22 +0.2 219,680 + rcsF b0196 0.22 +0.9 219,720 - rcsF b0196 0.32 -0.9 219,720 - rcsF b0196 0.32 -0.0 219,756 + rcsF b0196 0.41 +0.1 219,764 - rcsF b0196 0.43 +0.5 219,764 - rcsF b0196 0.43 +0.6 219,764 - rcsF b0196 0.43 +0.3 219,842 - rcsF b0196 0.62 -0.2 219,902 + rcsF b0196 0.77 +0.8 219,944 - rcsF b0196 0.87 +1.5 219,968 + +0.2 219,976 - +0.7 220,041 - +1.0 220,041 - +0.0 220,041 - +0.8 220,127 - -0.1 220,127 - +0.4 220,249 - metQ b0197 0.17 +0.3 220,321 + metQ b0197 0.25 -1.0 220,321 + metQ b0197 0.25 +0.8 220,323 + metQ b0197 0.26 -0.4 220,361 - metQ b0197 0.30 -0.1 220,386 - metQ b0197 0.33 -0.5 220,386 - metQ b0197 0.33 +0.6 220,405 + metQ b0197 0.36 -1.8 220,405 + metQ b0197 0.36 -0.4 220,432 + metQ b0197 0.39 +0.3 220,432 + metQ b0197 0.39 +0.1 220,504 + metQ b0197 0.48 -0.3 220,532 + metQ b0197 0.51 +1.1 220,543 + metQ b0197 0.53 -0.1 220,661 + metQ b0197 0.67 +0.1 220,675 + metQ b0197 0.69 +0.4 220,694 - metQ b0197 0.71 -0.2 220,694 - metQ b0197 0.71 -0.3 220,750 - metQ b0197 0.78 +0.1 220,754 + metQ b0197 0.79 -0.6 220,764 + metQ b0197 0.80 +0.4 220,825 + metQ b0197 0.87 -0.6 220,865 + -0.1 221,043 - metI b0198 0.11 -0.7 221,047 + metI b0198 0.12 +0.3 221,052 + metI b0198 0.13 +0.4 221,060 - metI b0198 0.14 -0.8 221,076 + metI b0198 0.17 +0.4 221,076 + metI b0198 0.17 +0.2 221,080 + metI b0198 0.17 -0.3 221,080 + metI b0198 0.17 +1.6 221,096 - metI b0198 0.20 -0.2 221,105 - metI b0198 0.21 -0.2 221,106 - metI b0198 0.21 -0.2 221,128 - metI b0198 0.24 -0.2 221,148 + metI b0198 0.28 +0.0 221,148 + metI b0198 0.28 +0.3 221,181 + metI b0198 0.33 -0.7 221,214 + metI b0198 0.38 +1.3 221,238 + metI b0198 0.41 -0.3 221,238 + metI b0198 0.41 -0.3 221,250 - metI b0198 0.43 +0.2 221,269 - metI b0198 0.46 -0.8 221,280 + metI b0198 0.48 +0.8 221,300 - metI b0198 0.51 -0.3 221,347 - metI b0198 0.58 +0.3 221,383 - metI b0198 0.63 -0.5 221,383 - metI b0198 0.63 -0.0 221,466 - metI b0198 0.76 +0.5 221,469 - metI b0198 0.77 +0.1 221,511 - metI b0198 0.83 +0.0 221,511 - metI b0198 0.83 +0.5 221,595 - +0.2 221,595 - -0.1 221,619 + -0.2 221,623 + +0.4 221,655 - -0.1 221,680 + -0.4 221,685 + -0.1 221,688 - -0.5 221,802 + metN b0199 0.18 +0.1 221,830 + metN b0199 0.21 -1.5 221,830 + metN b0199 0.21 +0.3 221,846 + metN b0199 0.22 +0.4 221,847 + metN b0199 0.23 -0.4 221,848 + metN b0199 0.23 +0.2 221,856 + metN b0199 0.23 +0.2 221,911 - metN b0199 0.29 -0.4
Or see this region's nucleotide sequence