Experiment: R2A control with 0.2X Vogels
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_23SD and PP_5SD are separated by 131 nucleotides PP_5SD and PP_0598 are separated by 258 nucleotides PP_0598 and PP_5745 overlap by 14 nucleotides
PP_23SD: PP_23SD - 23S ribosomal RNA, at 699,851 to 702,752
_23SD
PP_5SD: PP_5SD - 5S ribosomal RNA, at 702,884 to 703,003
_5SD
PP_0598: PP_0598 - paraquat-inducible protein A, at 703,262 to 703,921
_0598
PP_5745: PP_5745 - paraquat-inducible protein A, at 703,908 to 704,531
_5745
Position (kb) 702
703
704 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 703.138 kb on + strand at 703.226 kb on + strand at 703.226 kb on + strand at 703.227 kb on - strand at 703.227 kb on - strand at 703.232 kb on - strand at 703.232 kb on - strand at 703.246 kb on + strand at 703.247 kb on - strand at 703.247 kb on - strand at 703.247 kb on - strand at 703.249 kb on - strand at 703.261 kb on + strand at 703.261 kb on + strand at 703.261 kb on + strand at 703.262 kb on - strand at 703.262 kb on - strand at 703.407 kb on - strand, within PP_0598 at 703.407 kb on - strand, within PP_0598 at 703.407 kb on - strand, within PP_0598 at 703.407 kb on - strand, within PP_0598 at 703.407 kb on - strand, within PP_0598 at 703.531 kb on + strand, within PP_0598 at 703.551 kb on + strand, within PP_0598 at 703.551 kb on + strand at 703.552 kb on - strand, within PP_0598 at 703.552 kb on - strand, within PP_0598 at 703.559 kb on + strand, within PP_0598 at 703.559 kb on + strand, within PP_0598 at 703.559 kb on + strand, within PP_0598 at 703.559 kb on + strand, within PP_0598 at 703.560 kb on - strand, within PP_0598 at 703.560 kb on - strand, within PP_0598 at 703.560 kb on - strand, within PP_0598 at 703.593 kb on + strand, within PP_0598 at 703.593 kb on + strand, within PP_0598 at 703.593 kb on + strand, within PP_0598 at 703.593 kb on + strand, within PP_0598 at 703.593 kb on + strand, within PP_0598 at 703.594 kb on - strand, within PP_0598 at 703.594 kb on - strand, within PP_0598 at 703.594 kb on - strand, within PP_0598 at 703.594 kb on - strand, within PP_0598 at 703.603 kb on + strand, within PP_0598 at 703.603 kb on + strand, within PP_0598 at 703.603 kb on + strand, within PP_0598 at 703.603 kb on + strand, within PP_0598 at 703.604 kb on - strand, within PP_0598 at 703.604 kb on - strand, within PP_0598 at 703.604 kb on - strand, within PP_0598 at 703.604 kb on - strand, within PP_0598 at 703.682 kb on + strand, within PP_0598 at 703.682 kb on + strand, within PP_0598 at 703.683 kb on - strand, within PP_0598 at 703.698 kb on - strand, within PP_0598 at 703.706 kb on + strand, within PP_0598 at 703.707 kb on - strand at 703.742 kb on + strand, within PP_0598 at 703.834 kb on - strand, within PP_0598 at 703.834 kb on - strand, within PP_0598 at 703.834 kb on - strand, within PP_0598 at 703.834 kb on - strand, within PP_0598
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control with 0.2X Vogels remove 703,138 + -3.0 703,226 + +1.0 703,226 + -3.0 703,227 - -1.3 703,227 - +0.9 703,232 - -1.0 703,232 - -3.7 703,246 + -0.1 703,247 - +2.4 703,247 - +0.4 703,247 - +0.6 703,249 - -0.4 703,261 + -0.4 703,261 + +0.7 703,261 + +0.5 703,262 - +0.4 703,262 - -1.0 703,407 - PP_0598 0.22 +0.8 703,407 - PP_0598 0.22 +1.0 703,407 - PP_0598 0.22 -0.0 703,407 - PP_0598 0.22 +0.3 703,407 - PP_0598 0.22 -0.5 703,531 + PP_0598 0.41 +1.9 703,551 + PP_0598 0.44 -1.7 703,551 + -1.2 703,552 - PP_0598 0.44 +0.7 703,552 - PP_0598 0.44 -0.6 703,559 + PP_0598 0.45 +1.3 703,559 + PP_0598 0.45 +0.2 703,559 + PP_0598 0.45 +1.6 703,559 + PP_0598 0.45 +1.1 703,560 - PP_0598 0.45 +0.7 703,560 - PP_0598 0.45 -0.1 703,560 - PP_0598 0.45 +0.9 703,593 + PP_0598 0.50 +1.2 703,593 + PP_0598 0.50 -0.0 703,593 + PP_0598 0.50 +0.3 703,593 + PP_0598 0.50 +1.4 703,593 + PP_0598 0.50 +0.6 703,594 - PP_0598 0.50 +0.9 703,594 - PP_0598 0.50 +0.9 703,594 - PP_0598 0.50 +1.5 703,594 - PP_0598 0.50 +0.8 703,603 + PP_0598 0.52 +1.2 703,603 + PP_0598 0.52 +1.4 703,603 + PP_0598 0.52 +1.0 703,603 + PP_0598 0.52 +0.2 703,604 - PP_0598 0.52 +1.8 703,604 - PP_0598 0.52 +0.8 703,604 - PP_0598 0.52 +0.1 703,604 - PP_0598 0.52 +0.5 703,682 + PP_0598 0.64 +0.8 703,682 + PP_0598 0.64 +1.5 703,683 - PP_0598 0.64 -0.1 703,698 - PP_0598 0.66 +0.3 703,706 + PP_0598 0.67 +0.9 703,707 - -1.3 703,742 + PP_0598 0.73 +1.3 703,834 - PP_0598 0.87 +0.4 703,834 - PP_0598 0.87 +0.7 703,834 - PP_0598 0.87 +1.2 703,834 - PP_0598 0.87 -0.5
Or see this region's nucleotide sequence