Experiment: M9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rrlE and rrfE are separated by 93 nucleotides rrfE and yjaA are separated by 74 nucleotides yjaA and yjaB are separated by 62 nucleotides yjaB and metA are separated by 156 nucleotides
b4009: rrlE - 23S ribosomal RNA (NCBI), at 4,208,066 to 4,210,969
rrlE
b4010: rrfE - 5S ribosomal RNA (NCBI), at 4,211,063 to 4,211,182
rrfE
b4011: yjaA - hypothetical protein (NCBI), at 4,211,257 to 4,211,640
yjaA
b4012: yjaB - predicted acetyltransferase (NCBI), at 4,211,703 to 4,212,146
yjaB
b4013: metA - homoserine O-succinyltransferase (NCBI), at 4,212,303 to 4,213,232
metA
Position (kb) 4211
4212 Strain fitness (log2 ratio) -1
0
1 at 4211.358 kb on - strand, within yjaA at 4211.358 kb on - strand, within yjaA at 4211.402 kb on + strand, within yjaA at 4211.605 kb on - strand at 4211.659 kb on - strand at 4211.767 kb on - strand, within yjaB at 4211.973 kb on + strand, within yjaB at 4211.994 kb on - strand, within yjaB at 4211.994 kb on - strand, within yjaB at 4212.254 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 remove 4,211,358 - yjaA b4011 0.26 +0.1 4,211,358 - yjaA b4011 0.26 +0.2 4,211,402 + yjaA b4011 0.38 +0.2 4,211,605 - +0.0 4,211,659 - +0.1 4,211,767 - yjaB b4012 0.14 +0.2 4,211,973 + yjaB b4012 0.61 -0.0 4,211,994 - yjaB b4012 0.66 +0.4 4,211,994 - yjaB b4012 0.66 -0.4 4,212,254 - -0.5
Or see this region's nucleotide sequence