Experiment: M9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebL and znuC are separated by 78 nucleotides znuC and znuB overlap by 4 nucleotides znuB and ruvB are separated by 146 nucleotides
b1857: yebL - putative adhesin (VIMSS), at 1,939,675 to 1,940,607
yebL
b1858: znuC - high-affinity zinc transporter ATPase (NCBI), at 1,940,686 to 1,941,441
znuC
b1859: znuB - high-affinity zinc transporter membrane component (NCBI), at 1,941,438 to 1,942,223
znuB
b1860: ruvB - Holliday junction DNA helicase B (NCBI), at 1,942,370 to 1,943,380
ruvB
Position (kb)
1940
1941
1942 Strain fitness (log2 ratio)
-2
-1
0
1 at 1939.694 kb on + strand at 1939.813 kb on + strand, within yebL at 1939.826 kb on + strand, within yebL at 1939.826 kb on + strand, within yebL at 1939.834 kb on - strand, within yebL at 1939.834 kb on - strand, within yebL at 1939.834 kb on - strand, within yebL at 1940.017 kb on - strand, within yebL at 1940.017 kb on - strand, within yebL at 1940.087 kb on - strand, within yebL at 1940.087 kb on - strand, within yebL at 1940.238 kb on - strand, within yebL at 1940.238 kb on - strand, within yebL at 1940.554 kb on + strand at 1940.831 kb on - strand, within znuC at 1940.831 kb on - strand, within znuC at 1940.844 kb on + strand, within znuC at 1940.964 kb on + strand, within znuC at 1940.970 kb on - strand, within znuC at 1940.970 kb on - strand, within znuC at 1940.972 kb on - strand, within znuC at 1940.972 kb on - strand, within znuC at 1941.053 kb on - strand, within znuC at 1941.053 kb on - strand, within znuC at 1941.081 kb on - strand, within znuC at 1941.195 kb on + strand, within znuC at 1941.208 kb on - strand, within znuC at 1941.228 kb on + strand, within znuC at 1941.385 kb on - strand at 1941.492 kb on - strand at 1941.587 kb on + strand, within znuB at 1941.587 kb on + strand, within znuB at 1941.589 kb on + strand, within znuB at 1941.662 kb on + strand, within znuB at 1941.662 kb on + strand at 1941.704 kb on + strand, within znuB at 1941.712 kb on - strand at 1941.714 kb on + strand, within znuB at 1941.749 kb on + strand, within znuB at 1941.914 kb on + strand, within znuB at 1941.914 kb on + strand, within znuB at 1941.978 kb on + strand, within znuB at 1942.038 kb on + strand, within znuB at 1942.038 kb on + strand, within znuB at 1942.094 kb on + strand, within znuB at 1942.094 kb on + strand, within znuB at 1942.156 kb on - strand at 1942.156 kb on - strand at 1942.184 kb on + strand at 1942.295 kb on + strand at 1942.295 kb on + strand at 1942.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 remove 1,939,694 + -0.6 1,939,813 + yebL b1857 0.15 -1.6 1,939,826 + yebL b1857 0.16 -2.0 1,939,826 + yebL b1857 0.16 -2.7 1,939,834 - yebL b1857 0.17 -1.2 1,939,834 - yebL b1857 0.17 -1.4 1,939,834 - yebL b1857 0.17 -0.4 1,940,017 - yebL b1857 0.37 -0.6 1,940,017 - yebL b1857 0.37 -1.6 1,940,087 - yebL b1857 0.44 -2.4 1,940,087 - yebL b1857 0.44 -1.5 1,940,238 - yebL b1857 0.60 -1.9 1,940,238 - yebL b1857 0.60 -1.0 1,940,554 + -1.0 1,940,831 - znuC b1858 0.19 -0.8 1,940,831 - znuC b1858 0.19 -1.6 1,940,844 + znuC b1858 0.21 -1.0 1,940,964 + znuC b1858 0.37 -1.9 1,940,970 - znuC b1858 0.38 -1.1 1,940,970 - znuC b1858 0.38 -2.7 1,940,972 - znuC b1858 0.38 -1.5 1,940,972 - znuC b1858 0.38 -0.3 1,941,053 - znuC b1858 0.49 -1.2 1,941,053 - znuC b1858 0.49 -1.1 1,941,081 - znuC b1858 0.52 -0.1 1,941,195 + znuC b1858 0.67 -0.7 1,941,208 - znuC b1858 0.69 -2.6 1,941,228 + znuC b1858 0.72 -1.1 1,941,385 - -0.8 1,941,492 - -1.9 1,941,587 + znuB b1859 0.19 -0.7 1,941,587 + znuB b1859 0.19 -1.1 1,941,589 + znuB b1859 0.19 -0.3 1,941,662 + znuB b1859 0.28 -1.8 1,941,662 + -1.6 1,941,704 + znuB b1859 0.34 -0.2 1,941,712 - +0.4 1,941,714 + znuB b1859 0.35 -2.0 1,941,749 + znuB b1859 0.40 -1.4 1,941,914 + znuB b1859 0.61 -1.2 1,941,914 + znuB b1859 0.61 -1.0 1,941,978 + znuB b1859 0.69 -1.7 1,942,038 + znuB b1859 0.76 -0.7 1,942,038 + znuB b1859 0.76 -1.6 1,942,094 + znuB b1859 0.83 -1.5 1,942,094 + znuB b1859 0.83 -1.0 1,942,156 - +0.8 1,942,156 - -2.1 1,942,184 + +0.4 1,942,295 + -0.4 1,942,295 + -1.0 1,942,423 + -0.3
Or see this region's nucleotide sequence