Experiment: M9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpB and trpC are separated by 11 nucleotides trpC and trpD are separated by 3 nucleotides
b1261: trpB - tryptophan synthase subunit beta (NCBI), at 1,315,246 to 1,316,439
trpB
b1262: trpC - N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS), at 1,316,451 to 1,317,809
trpC
b1263: trpD - bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI), at 1,317,813 to 1,319,408
trpD
Position (kb)
1316
1317
1318 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1315.453 kb on + strand, within trpB at 1315.671 kb on + strand, within trpB at 1315.984 kb on - strand, within trpB at 1316.375 kb on - strand at 1316.375 kb on - strand at 1316.600 kb on + strand, within trpC at 1316.692 kb on + strand, within trpC at 1316.692 kb on + strand, within trpC at 1317.035 kb on - strand, within trpC at 1317.035 kb on - strand, within trpC at 1317.113 kb on - strand, within trpC at 1317.144 kb on - strand, within trpC at 1317.153 kb on + strand, within trpC at 1317.181 kb on + strand, within trpC at 1317.305 kb on - strand, within trpC at 1317.389 kb on + strand, within trpC at 1317.389 kb on + strand, within trpC at 1317.643 kb on + strand, within trpC at 1317.684 kb on - strand at 1317.684 kb on - strand at 1317.766 kb on - strand at 1318.316 kb on + strand, within trpD at 1318.316 kb on + strand, within trpD
Per-strain Table
Position Strand Gene LocusTag Fraction M9 remove 1,315,453 + trpB b1261 0.17 -4.0 1,315,671 + trpB b1261 0.36 -3.7 1,315,984 - trpB b1261 0.62 -3.1 1,316,375 - -2.6 1,316,375 - -2.9 1,316,600 + trpC b1262 0.11 -3.7 1,316,692 + trpC b1262 0.18 -2.8 1,316,692 + trpC b1262 0.18 -1.1 1,317,035 - trpC b1262 0.43 +0.0 1,317,035 - trpC b1262 0.43 -0.3 1,317,113 - trpC b1262 0.49 +0.9 1,317,144 - trpC b1262 0.51 -0.3 1,317,153 + trpC b1262 0.52 -2.7 1,317,181 + trpC b1262 0.54 -2.5 1,317,305 - trpC b1262 0.63 -0.9 1,317,389 + trpC b1262 0.69 -3.0 1,317,389 + trpC b1262 0.69 -2.2 1,317,643 + trpC b1262 0.88 -3.4 1,317,684 - -0.2 1,317,684 - -1.2 1,317,766 - -0.3 1,318,316 + trpD b1263 0.32 -0.4 1,318,316 + trpD b1263 0.32 -0.9
Or see this region's nucleotide sequence