Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0069 and PP_0070 are separated by 42 nucleotides PP_0070 and PP_5425 are separated by 133 nucleotides PP_5425 and PP_0071 are separated by 413 nucleotides PP_0071 and PP_0072 are separated by 127 nucleotides
PP_0069: PP_0069 - putative Rossmann fold nucleotide-binding protein, at 79,246 to 80,343
_0069
PP_0070: PP_0070 - Threonylcarbamoyl-AMP synthase, at 80,386 to 80,943
_0070
PP_5425: PP_5425 - conserved protein of unknown function, at 81,077 to 81,277
_5425
PP_0071: PP_0071 - conserved protein of unknown function, at 81,691 to 82,023
_0071
PP_0072: PP_0072 - quinone oxidoreductase, at 82,151 to 83,128
_0072
Position (kb)
81
82 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 80.341 kb on + strand at 80.341 kb on + strand at 80.345 kb on + strand at 80.345 kb on + strand at 80.346 kb on - strand at 80.346 kb on - strand at 80.346 kb on - strand at 80.365 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.945 kb on - strand at 80.945 kb on - strand at 81.007 kb on + strand at 81.007 kb on + strand at 81.007 kb on + strand at 81.116 kb on + strand, within PP_5425 at 81.116 kb on + strand, within PP_5425 at 81.116 kb on + strand, within PP_5425 at 81.117 kb on - strand, within PP_5425 at 81.183 kb on - strand, within PP_5425 at 81.183 kb on - strand, within PP_5425 at 81.205 kb on + strand, within PP_5425 at 81.206 kb on - strand, within PP_5425 at 81.206 kb on - strand, within PP_5425 at 81.244 kb on - strand, within PP_5425 at 81.244 kb on - strand, within PP_5425 at 81.270 kb on + strand at 81.271 kb on - strand at 81.318 kb on + strand at 81.334 kb on + strand at 81.334 kb on + strand at 81.368 kb on + strand at 81.369 kb on - strand at 81.369 kb on - strand at 81.369 kb on - strand at 81.391 kb on + strand at 81.392 kb on - strand at 81.451 kb on + strand at 81.451 kb on + strand at 81.452 kb on - strand at 81.456 kb on + strand at 81.457 kb on - strand at 81.457 kb on - strand at 81.505 kb on + strand at 81.506 kb on - strand at 81.548 kb on - strand at 81.618 kb on + strand at 81.636 kb on + strand at 81.648 kb on + strand at 81.649 kb on - strand at 81.649 kb on - strand at 81.673 kb on - strand at 81.704 kb on + strand at 81.704 kb on + strand at 81.704 kb on + strand at 81.705 kb on - strand at 81.705 kb on - strand at 81.705 kb on - strand at 81.705 kb on - strand at 81.737 kb on + strand, within PP_0071 at 81.839 kb on + strand, within PP_0071 at 81.839 kb on + strand, within PP_0071 at 81.840 kb on - strand, within PP_0071 at 81.840 kb on - strand, within PP_0071 at 81.845 kb on + strand, within PP_0071 at 81.845 kb on + strand, within PP_0071 at 81.846 kb on - strand, within PP_0071 at 81.846 kb on - strand, within PP_0071 at 81.847 kb on + strand, within PP_0071 at 81.848 kb on - strand, within PP_0071 at 81.848 kb on - strand, within PP_0071 at 81.848 kb on - strand, within PP_0071 at 81.848 kb on - strand, within PP_0071 at 81.848 kb on - strand, within PP_0071 at 81.862 kb on + strand, within PP_0071 at 81.969 kb on - strand, within PP_0071 at 82.022 kb on - strand at 82.047 kb on + strand at 82.047 kb on + strand at 82.120 kb on + strand at 82.132 kb on + strand at 82.133 kb on - strand at 82.163 kb on + strand at 82.163 kb on + strand at 82.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 80,341 + +1.0 80,341 + +0.5 80,345 + +1.5 80,345 + +0.2 80,346 - -1.5 80,346 - -0.3 80,346 - +1.0 80,365 + +0.3 80,944 + -0.6 80,944 + -0.1 80,944 + -0.8 80,944 + -0.1 80,944 + -0.7 80,945 - +0.6 80,945 - -1.6 81,007 + +0.2 81,007 + -0.4 81,007 + -0.5 81,116 + PP_5425 0.19 -0.3 81,116 + PP_5425 0.19 -0.7 81,116 + PP_5425 0.19 -0.7 81,117 - PP_5425 0.20 -1.1 81,183 - PP_5425 0.53 -1.3 81,183 - PP_5425 0.53 -1.8 81,205 + PP_5425 0.64 -1.1 81,206 - PP_5425 0.64 -0.5 81,206 - PP_5425 0.64 +1.1 81,244 - PP_5425 0.83 -1.0 81,244 - PP_5425 0.83 -1.5 81,270 + +0.4 81,271 - -1.2 81,318 + -0.8 81,334 + +0.0 81,334 + +0.1 81,368 + +0.1 81,369 - -0.1 81,369 - +0.5 81,369 - +0.1 81,391 + -0.1 81,392 - -1.2 81,451 + +0.2 81,451 + +1.5 81,452 - -0.7 81,456 + +0.2 81,457 - -0.2 81,457 - +0.7 81,505 + +0.7 81,506 - +0.9 81,548 - -1.1 81,618 + -1.3 81,636 + +0.3 81,648 + +0.4 81,649 - -0.4 81,649 - -0.6 81,673 - -0.6 81,704 + -1.2 81,704 + +0.8 81,704 + -0.2 81,705 - +0.0 81,705 - -0.7 81,705 - +1.2 81,705 - -0.3 81,737 + PP_0071 0.14 +0.3 81,839 + PP_0071 0.44 +0.2 81,839 + PP_0071 0.44 +1.8 81,840 - PP_0071 0.45 +1.0 81,840 - PP_0071 0.45 -0.3 81,845 + PP_0071 0.46 -1.2 81,845 + PP_0071 0.46 -1.6 81,846 - PP_0071 0.47 -0.1 81,846 - PP_0071 0.47 -0.2 81,847 + PP_0071 0.47 -0.4 81,848 - PP_0071 0.47 +0.1 81,848 - PP_0071 0.47 +0.9 81,848 - PP_0071 0.47 -0.5 81,848 - PP_0071 0.47 +0.4 81,848 - PP_0071 0.47 +1.1 81,862 + PP_0071 0.51 -0.2 81,969 - PP_0071 0.83 +0.3 82,022 - +1.0 82,047 + +0.2 82,047 + +1.2 82,120 + -1.0 82,132 + -0.9 82,133 - -1.7 82,163 + +0.2 82,163 + -0.1 82,164 - +0.1
Or see this region's nucleotide sequence