Strain Fitness in Escherichia coli BW25113 around b4024

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjbC and yjbD are separated by 132 nucleotidesyjbD and lysC are separated by 252 nucleotideslysC and pgi are separated by 524 nucleotides b4022: yjbC - 23S rRNA pseudouridine synthase (NCBI), at 4,228,377 to 4,229,249 yjbC b4023: yjbD - hypothetical protein (NCBI), at 4,229,382 to 4,229,654 yjbD b4024: lysC - aspartate kinase III (NCBI), at 4,229,907 to 4,231,256 lysC b4025: pgi - glucose-6-phosphate isomerase (NCBI), at 4,231,781 to 4,233,430 pgi Position (kb) 4229 4230 4231 4232Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 4228.909 kb on + strand, within yjbCat 4228.915 kb on + strand, within yjbCat 4228.920 kb on - strand, within yjbCat 4228.923 kb on - strand, within yjbCat 4228.923 kb on - strand, within yjbCat 4228.929 kb on - strand, within yjbCat 4229.003 kb on - strand, within yjbCat 4229.003 kb on - strand, within yjbCat 4229.010 kb on + strand, within yjbCat 4229.010 kb on + strand, within yjbCat 4229.020 kb on + strand, within yjbCat 4229.020 kb on + strand, within yjbCat 4229.071 kb on + strand, within yjbCat 4229.072 kb on + strand, within yjbCat 4229.080 kb on - strand, within yjbCat 4229.080 kb on - strand, within yjbCat 4229.080 kb on - strand, within yjbCat 4229.154 kb on + strand, within yjbCat 4229.162 kb on - strandat 4229.255 kb on + strandat 4229.263 kb on - strandat 4229.263 kb on - strandat 4229.298 kb on - strandat 4229.298 kb on - strandat 4229.336 kb on + strandat 4229.421 kb on - strand, within yjbDat 4229.533 kb on + strand, within yjbDat 4229.539 kb on + strand, within yjbDat 4229.551 kb on + strand, within yjbDat 4229.555 kb on + strand, within yjbDat 4229.555 kb on + strand, within yjbDat 4229.563 kb on - strand, within yjbDat 4229.563 kb on - strand, within yjbDat 4229.563 kb on - strand, within yjbDat 4229.572 kb on + strand, within yjbDat 4229.583 kb on + strand, within yjbDat 4229.583 kb on + strand, within yjbDat 4229.591 kb on + strand, within yjbDat 4229.599 kb on + strand, within yjbDat 4229.603 kb on - strand, within yjbDat 4229.603 kb on - strand, within yjbDat 4229.609 kb on + strand, within yjbDat 4229.620 kb on + strand, within yjbDat 4229.638 kb on + strandat 4229.638 kb on + strandat 4229.638 kb on + strandat 4229.646 kb on - strandat 4229.648 kb on - strandat 4229.648 kb on - strandat 4229.650 kb on + strandat 4229.650 kb on + strandat 4229.650 kb on + strandat 4229.650 kb on + strandat 4229.651 kb on + strandat 4229.651 kb on + strandat 4229.659 kb on - strandat 4229.661 kb on + strandat 4229.663 kb on + strandat 4229.666 kb on + strandat 4229.668 kb on - strandat 4229.678 kb on + strandat 4229.693 kb on - strandat 4229.695 kb on - strandat 4229.734 kb on + strandat 4229.796 kb on + strandat 4229.818 kb on + strandat 4229.821 kb on - strandat 4229.826 kb on + strandat 4229.826 kb on - strandat 4229.831 kb on - strandat 4229.831 kb on - strandat 4229.831 kb on - strandat 4229.835 kb on - strandat 4229.836 kb on - strandat 4229.836 kb on - strandat 4229.836 kb on - strandat 4229.836 kb on - strandat 4229.845 kb on + strandat 4229.898 kb on + strandat 4229.898 kb on + strandat 4229.903 kb on + strandat 4229.903 kb on + strandat 4229.903 kb on + strandat 4229.910 kb on - strandat 4229.912 kb on - strandat 4229.951 kb on - strandat 4229.951 kb on - strandat 4229.953 kb on - strandat 4229.953 kb on - strandat 4229.964 kb on - strandat 4229.973 kb on + strandat 4229.973 kb on + strandat 4229.974 kb on - strandat 4229.978 kb on + strandat 4229.986 kb on - strandat 4229.986 kb on - strandat 4229.986 kb on - strandat 4229.997 kb on - strandat 4230.031 kb on - strandat 4230.031 kb on - strandat 4230.061 kb on + strand, within lysCat 4230.084 kb on - strand, within lysCat 4230.126 kb on - strand, within lysCat 4230.182 kb on - strand, within lysCat 4230.182 kb on - strand, within lysCat 4230.187 kb on - strand, within lysCat 4230.226 kb on - strand, within lysCat 4230.226 kb on - strand, within lysCat 4230.226 kb on - strand, within lysCat 4230.226 kb on - strand, within lysCat 4230.229 kb on + strand, within lysCat 4230.237 kb on - strand, within lysCat 4230.303 kb on - strand, within lysCat 4230.323 kb on - strand, within lysCat 4230.323 kb on - strand, within lysCat 4230.329 kb on - strand, within lysCat 4230.329 kb on - strand, within lysCat 4230.395 kb on + strand, within lysCat 4230.395 kb on + strand, within lysCat 4230.395 kb on + strand, within lysCat 4230.396 kb on + strand, within lysCat 4230.426 kb on + strand, within lysCat 4230.437 kb on - strand, within lysCat 4230.443 kb on - strand, within lysCat 4230.450 kb on + strand, within lysCat 4230.460 kb on - strand, within lysCat 4230.460 kb on - strand, within lysCat 4230.571 kb on + strand, within lysCat 4230.576 kb on + strand, within lysCat 4230.605 kb on + strand, within lysCat 4230.626 kb on - strand, within lysCat 4230.674 kb on - strand, within lysCat 4230.731 kb on + strand, within lysCat 4230.731 kb on + strand, within lysCat 4230.783 kb on + strand, within lysCat 4230.788 kb on + strand, within lysCat 4230.843 kb on - strand, within lysCat 4231.007 kb on - strand, within lysCat 4231.015 kb on + strand, within lysCat 4231.126 kb on - strandat 4231.140 kb on - strandat 4231.140 kb on - strandat 4231.194 kb on + strandat 4231.194 kb on + strandat 4231.200 kb on - strandat 4231.224 kb on - strandat 4231.224 kb on - strandat 4231.254 kb on + strandat 4231.254 kb on + strandat 4231.293 kb on + strandat 4231.331 kb on + strandat 4231.446 kb on - strandat 4231.485 kb on - strandat 4231.499 kb on + strandat 4231.524 kb on - strandat 4231.524 kb on - strandat 4231.570 kb on + strandat 4231.570 kb on + strandat 4231.590 kb on + strandat 4231.699 kb on - strandat 4231.859 kb on - strandat 4231.936 kb on - strandat 4231.936 kb on - strandat 4231.968 kb on - strand, within pgiat 4232.022 kb on - strand, within pgiat 4232.033 kb on + strand, within pgiat 4232.074 kb on - strand, within pgiat 4232.074 kb on - strand, within pgiat 4232.074 kb on - strand, within pgiat 4232.137 kb on + strand, within pgiat 4232.145 kb on - strand, within pgiat 4232.200 kb on + strand, within pgiat 4232.200 kb on + strand, within pgiat 4232.208 kb on - strand, within pgi

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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4,228,909 + yjbC b4022 0.61 -0.1
4,228,915 + yjbC b4022 0.62 +0.0
4,228,920 - yjbC b4022 0.62 -1.1
4,228,923 - yjbC b4022 0.63 +0.0
4,228,923 - yjbC b4022 0.63 +1.1
4,228,929 - yjbC b4022 0.63 -0.6
4,229,003 - yjbC b4022 0.72 -1.4
4,229,003 - yjbC b4022 0.72 -0.3
4,229,010 + yjbC b4022 0.73 +0.8
4,229,010 + yjbC b4022 0.73 +0.7
4,229,020 + yjbC b4022 0.74 +0.1
4,229,020 + yjbC b4022 0.74 +0.4
4,229,071 + yjbC b4022 0.79 -0.7
4,229,072 + yjbC b4022 0.80 -0.7
4,229,080 - yjbC b4022 0.81 +0.7
4,229,080 - yjbC b4022 0.81 +0.4
4,229,080 - yjbC b4022 0.81 -0.0
4,229,154 + yjbC b4022 0.89 -0.4
4,229,162 - -0.0
4,229,255 + -1.0
4,229,263 - +0.4
4,229,263 - -0.4
4,229,298 - +0.3
4,229,298 - -0.0
4,229,336 + -0.3
4,229,421 - yjbD b4023 0.14 -0.5
4,229,533 + yjbD b4023 0.55 -0.8
4,229,539 + yjbD b4023 0.58 -1.1
4,229,551 + yjbD b4023 0.62 +0.3
4,229,555 + yjbD b4023 0.63 -0.1
4,229,555 + yjbD b4023 0.63 -0.0
4,229,563 - yjbD b4023 0.66 -0.4
4,229,563 - yjbD b4023 0.66 +0.1
4,229,563 - yjbD b4023 0.66 -1.4
4,229,572 + yjbD b4023 0.70 -0.6
4,229,583 + yjbD b4023 0.74 -1.7
4,229,583 + yjbD b4023 0.74 -0.3
4,229,591 + yjbD b4023 0.77 +0.3
4,229,599 + yjbD b4023 0.79 +0.4
4,229,603 - yjbD b4023 0.81 -0.4
4,229,603 - yjbD b4023 0.81 +0.7
4,229,609 + yjbD b4023 0.83 +1.3
4,229,620 + yjbD b4023 0.87 -0.2
4,229,638 + -1.3
4,229,638 + +0.3
4,229,638 + +0.8
4,229,646 - +0.3
4,229,648 - -0.3
4,229,648 - +0.1
4,229,650 + +0.6
4,229,650 + -0.3
4,229,650 + +0.7
4,229,650 + -0.7
4,229,651 + +0.2
4,229,651 + -0.3
4,229,659 - -0.7
4,229,661 + +0.5
4,229,663 + +0.2
4,229,666 + -0.5
4,229,668 - +0.1
4,229,678 + -0.2
4,229,693 - +3.1
4,229,695 - +4.1
4,229,734 + +0.9
4,229,796 + +1.1
4,229,818 + -0.2
4,229,821 - +0.1
4,229,826 + -0.1
4,229,826 - -0.2
4,229,831 - +0.2
4,229,831 - -0.9
4,229,831 - +0.2
4,229,835 - +0.2
4,229,836 - -0.2
4,229,836 - -1.2
4,229,836 - +0.5
4,229,836 - -0.1
4,229,845 + +1.5
4,229,898 + +1.1
4,229,898 + +4.1
4,229,903 + +0.1
4,229,903 + +2.1
4,229,903 + +0.4
4,229,910 - -0.4
4,229,912 - -0.1
4,229,951 - +0.3
4,229,951 - -0.3
4,229,953 - -0.3
4,229,953 - -0.6
4,229,964 - -0.6
4,229,973 + -0.7
4,229,973 + -0.7
4,229,974 - -0.1
4,229,978 + -0.9
4,229,986 - -1.2
4,229,986 - +0.3
4,229,986 - +0.1
4,229,997 - +0.2
4,230,031 - -0.1
4,230,031 - +0.0
4,230,061 + lysC b4024 0.11 -1.4
4,230,084 - lysC b4024 0.13 -1.1
4,230,126 - lysC b4024 0.16 +0.2
4,230,182 - lysC b4024 0.20 +1.0
4,230,182 - lysC b4024 0.20 +0.1
4,230,187 - lysC b4024 0.21 +0.1
4,230,226 - lysC b4024 0.24 -0.2
4,230,226 - lysC b4024 0.24 +0.5
4,230,226 - lysC b4024 0.24 -0.1
4,230,226 - lysC b4024 0.24 +0.1
4,230,229 + lysC b4024 0.24 +0.0
4,230,237 - lysC b4024 0.24 -1.4
4,230,303 - lysC b4024 0.29 -0.7
4,230,323 - lysC b4024 0.31 +0.7
4,230,323 - lysC b4024 0.31 +1.0
4,230,329 - lysC b4024 0.31 -0.6
4,230,329 - lysC b4024 0.31 +0.7
4,230,395 + lysC b4024 0.36 -0.0
4,230,395 + lysC b4024 0.36 -0.9
4,230,395 + lysC b4024 0.36 +0.3
4,230,396 + lysC b4024 0.36 -0.3
4,230,426 + lysC b4024 0.38 +0.2
4,230,437 - lysC b4024 0.39 +2.7
4,230,443 - lysC b4024 0.40 +0.1
4,230,450 + lysC b4024 0.40 +0.4
4,230,460 - lysC b4024 0.41 -0.4
4,230,460 - lysC b4024 0.41 +0.3
4,230,571 + lysC b4024 0.49 +0.4
4,230,576 + lysC b4024 0.50 +0.4
4,230,605 + lysC b4024 0.52 -0.3
4,230,626 - lysC b4024 0.53 -0.3
4,230,674 - lysC b4024 0.57 -0.6
4,230,731 + lysC b4024 0.61 +0.8
4,230,731 + lysC b4024 0.61 -0.5
4,230,783 + lysC b4024 0.65 -0.2
4,230,788 + lysC b4024 0.65 -0.4
4,230,843 - lysC b4024 0.69 -0.3
4,231,007 - lysC b4024 0.81 -0.1
4,231,015 + lysC b4024 0.82 +0.6
4,231,126 - +0.3
4,231,140 - -0.9
4,231,140 - -0.4
4,231,194 + +0.8
4,231,194 + -0.6
4,231,200 - +2.1
4,231,224 - -0.3
4,231,224 - -0.2
4,231,254 + +0.3
4,231,254 + +0.4
4,231,293 + +1.1
4,231,331 + -0.5
4,231,446 - -0.4
4,231,485 - -1.5
4,231,499 + +0.1
4,231,524 - -0.1
4,231,524 - +0.1
4,231,570 + -0.9
4,231,570 + -0.1
4,231,590 + +0.2
4,231,699 - +0.1
4,231,859 - -1.0
4,231,936 - -0.4
4,231,936 - -0.3
4,231,968 - pgi b4025 0.11 -2.0
4,232,022 - pgi b4025 0.15 -0.3
4,232,033 + pgi b4025 0.15 -0.2
4,232,074 - pgi b4025 0.18 -1.3
4,232,074 - pgi b4025 0.18 -3.8
4,232,074 - pgi b4025 0.18 -0.9
4,232,137 + pgi b4025 0.22 -1.3
4,232,145 - pgi b4025 0.22 -0.4
4,232,200 + pgi b4025 0.25 -1.6
4,232,200 + pgi b4025 0.25 -1.4
4,232,208 - pgi b4025 0.26 -1.1

Or see this region's nucleotide sequence