Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcaR and hcaE are separated by 135 nucleotides hcaE and hcaF overlap by 4 nucleotides hcaF and hcaC overlap by 1 nucleotides hcaC and hcaB overlap by 4 nucleotides
b2537: hcaR - DNA-binding transcriptional activator of 3-phenylpropionic acid catabolism (NCBI), at 2,666,028 to 2,666,918
hcaR
b2538: hcaE - 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI), at 2,667,054 to 2,668,415
hcaE
b2539: hcaF - 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI), at 2,668,412 to 2,668,930
hcaF
b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250
hcaC
b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059
hcaB
Position (kb)
2667
2668
2669 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 2666.111 kb on - strand at 2666.203 kb on - strand, within hcaR at 2666.213 kb on + strand, within hcaR at 2666.213 kb on + strand, within hcaR at 2666.216 kb on - strand, within hcaR at 2666.269 kb on - strand, within hcaR at 2666.334 kb on - strand, within hcaR at 2666.359 kb on + strand, within hcaR at 2666.361 kb on + strand, within hcaR at 2666.389 kb on - strand, within hcaR at 2666.389 kb on - strand, within hcaR at 2666.551 kb on + strand, within hcaR at 2666.563 kb on + strand, within hcaR at 2666.824 kb on + strand, within hcaR at 2666.839 kb on - strand at 2666.877 kb on + strand at 2666.877 kb on + strand at 2666.931 kb on - strand at 2667.028 kb on - strand at 2667.028 kb on - strand at 2667.079 kb on + strand at 2667.079 kb on + strand at 2667.364 kb on - strand, within hcaE at 2667.364 kb on - strand, within hcaE at 2667.536 kb on + strand, within hcaE at 2667.646 kb on - strand, within hcaE at 2667.730 kb on - strand, within hcaE at 2667.827 kb on - strand, within hcaE at 2667.880 kb on - strand, within hcaE at 2667.930 kb on - strand, within hcaE at 2667.956 kb on + strand, within hcaE at 2668.118 kb on - strand, within hcaE at 2668.174 kb on - strand, within hcaE at 2668.253 kb on + strand, within hcaE at 2668.253 kb on + strand, within hcaE at 2668.363 kb on + strand at 2668.539 kb on - strand, within hcaF at 2668.551 kb on - strand, within hcaF at 2668.551 kb on - strand, within hcaF at 2668.676 kb on + strand, within hcaF at 2668.676 kb on + strand, within hcaF at 2668.709 kb on + strand, within hcaF at 2668.715 kb on + strand, within hcaF at 2668.736 kb on - strand, within hcaF at 2668.759 kb on + strand, within hcaF at 2668.888 kb on - strand at 2668.888 kb on - strand at 2668.925 kb on - strand at 2668.932 kb on - strand at 2668.979 kb on - strand, within hcaC at 2669.083 kb on + strand, within hcaC at 2669.112 kb on - strand, within hcaC at 2669.166 kb on + strand, within hcaC at 2669.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,666,111 - +0.5 2,666,203 - hcaR b2537 0.20 +0.2 2,666,213 + hcaR b2537 0.21 -0.3 2,666,213 + hcaR b2537 0.21 -1.1 2,666,216 - hcaR b2537 0.21 -0.6 2,666,269 - hcaR b2537 0.27 -0.2 2,666,334 - hcaR b2537 0.34 -0.2 2,666,359 + hcaR b2537 0.37 +0.0 2,666,361 + hcaR b2537 0.37 +0.1 2,666,389 - hcaR b2537 0.41 -0.5 2,666,389 - hcaR b2537 0.41 +0.1 2,666,551 + hcaR b2537 0.59 -0.1 2,666,563 + hcaR b2537 0.60 -0.5 2,666,824 + hcaR b2537 0.89 -0.6 2,666,839 - +0.4 2,666,877 + -0.9 2,666,877 + +0.1 2,666,931 - +0.3 2,667,028 - +0.5 2,667,028 - -0.2 2,667,079 + -0.5 2,667,079 + +0.5 2,667,364 - hcaE b2538 0.23 -0.1 2,667,364 - hcaE b2538 0.23 -0.7 2,667,536 + hcaE b2538 0.35 +0.6 2,667,646 - hcaE b2538 0.43 -0.0 2,667,730 - hcaE b2538 0.50 -0.1 2,667,827 - hcaE b2538 0.57 -0.4 2,667,880 - hcaE b2538 0.61 +0.8 2,667,930 - hcaE b2538 0.64 +0.9 2,667,956 + hcaE b2538 0.66 +0.3 2,668,118 - hcaE b2538 0.78 +0.0 2,668,174 - hcaE b2538 0.82 -0.8 2,668,253 + hcaE b2538 0.88 +2.2 2,668,253 + hcaE b2538 0.88 +5.8 2,668,363 + +0.2 2,668,539 - hcaF b2539 0.24 -0.2 2,668,551 - hcaF b2539 0.27 -0.7 2,668,551 - hcaF b2539 0.27 -0.0 2,668,676 + hcaF b2539 0.51 -0.4 2,668,676 + hcaF b2539 0.51 +1.4 2,668,709 + hcaF b2539 0.57 -1.9 2,668,715 + hcaF b2539 0.58 -0.9 2,668,736 - hcaF b2539 0.62 -0.9 2,668,759 + hcaF b2539 0.67 +0.2 2,668,888 - -0.6 2,668,888 - +0.2 2,668,925 - +0.1 2,668,932 - +0.3 2,668,979 - hcaC b2540 0.15 -0.3 2,669,083 + hcaC b2540 0.48 -1.4 2,669,112 - hcaC b2540 0.57 +0.5 2,669,166 + hcaC b2540 0.74 -0.2 2,669,295 + -0.9
Or see this region's nucleotide sequence