Strain Fitness in Escherichia coli BW25113 around b2717

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntascB and hycI are separated by 158 nucleotideshycI and hycH overlap by 8 nucleotideshycH and hycG overlap by 4 nucleotides b2716: ascB - cryptic 6-phospho-beta-glucosidase (NCBI), at 2,839,012 to 2,840,436 ascB b2717: hycI - protease involved in processing C-terminal end of HycE (NCBI), at 2,840,595 to 2,841,065 hycI b2718: hycH - protein required for maturation of hydrogenase 3 (NCBI), at 2,841,058 to 2,841,468 hycH b2719: hycG - hydrogenase 3 and formate hydrogenase complex, HycG subunit (NCBI), at 2,841,465 to 2,842,232 hycG Position (kb) 2840 2841 2842Strain fitness (log2 ratio) -2 -1 0 1at 2839.607 kb on + strand, within ascBat 2839.661 kb on - strand, within ascBat 2839.893 kb on - strand, within ascBat 2839.893 kb on - strand, within ascBat 2840.238 kb on + strand, within ascBat 2840.256 kb on - strand, within ascBat 2840.263 kb on + strand, within ascBat 2840.281 kb on - strand, within ascBat 2840.497 kb on - strandat 2840.551 kb on - strandat 2840.558 kb on + strandat 2840.644 kb on + strand, within hycIat 2840.688 kb on - strand, within hycIat 2840.699 kb on + strand, within hycIat 2840.699 kb on + strand, within hycIat 2840.711 kb on - strand, within hycIat 2840.711 kb on - strand, within hycIat 2840.731 kb on - strand, within hycIat 2840.731 kb on - strand, within hycIat 2841.008 kb on + strand, within hycIat 2841.071 kb on + strandat 2841.119 kb on + strand, within hycHat 2841.150 kb on + strand, within hycHat 2841.167 kb on + strand, within hycHat 2841.175 kb on - strand, within hycHat 2841.207 kb on + strand, within hycHat 2841.225 kb on + strand, within hycHat 2841.272 kb on - strand, within hycHat 2841.300 kb on + strand, within hycHat 2841.300 kb on + strand, within hycHat 2841.327 kb on - strand, within hycHat 2841.327 kb on - strand, within hycHat 2841.338 kb on - strand, within hycHat 2841.384 kb on - strand, within hycHat 2841.572 kb on + strand, within hycGat 2841.581 kb on - strand, within hycGat 2841.622 kb on - strand, within hycGat 2841.645 kb on - strand, within hycGat 2841.648 kb on - strand, within hycGat 2841.713 kb on + strand, within hycGat 2841.774 kb on - strand, within hycGat 2841.821 kb on - strand, within hycGat 2841.861 kb on - strand, within hycGat 2841.870 kb on - strand, within hycGat 2842.019 kb on + strand, within hycGat 2842.047 kb on - strand, within hycG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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2,839,607 + ascB b2716 0.42 +0.4
2,839,661 - ascB b2716 0.46 +0.7
2,839,893 - ascB b2716 0.62 -0.4
2,839,893 - ascB b2716 0.62 -0.1
2,840,238 + ascB b2716 0.86 +0.1
2,840,256 - ascB b2716 0.87 -0.2
2,840,263 + ascB b2716 0.88 +0.7
2,840,281 - ascB b2716 0.89 +0.2
2,840,497 - -0.2
2,840,551 - -0.8
2,840,558 + +0.0
2,840,644 + hycI b2717 0.10 -0.6
2,840,688 - hycI b2717 0.20 -0.5
2,840,699 + hycI b2717 0.22 -0.5
2,840,699 + hycI b2717 0.22 -1.1
2,840,711 - hycI b2717 0.25 -0.1
2,840,711 - hycI b2717 0.25 -0.4
2,840,731 - hycI b2717 0.29 -0.4
2,840,731 - hycI b2717 0.29 -0.3
2,841,008 + hycI b2717 0.88 -0.2
2,841,071 + +0.1
2,841,119 + hycH b2718 0.15 +0.4
2,841,150 + hycH b2718 0.22 +0.6
2,841,167 + hycH b2718 0.27 -0.3
2,841,175 - hycH b2718 0.28 +1.1
2,841,207 + hycH b2718 0.36 -0.3
2,841,225 + hycH b2718 0.41 -0.6
2,841,272 - hycH b2718 0.52 -0.3
2,841,300 + hycH b2718 0.59 -0.1
2,841,300 + hycH b2718 0.59 -0.2
2,841,327 - hycH b2718 0.65 -2.1
2,841,327 - hycH b2718 0.65 -0.2
2,841,338 - hycH b2718 0.68 -0.5
2,841,384 - hycH b2718 0.79 +1.3
2,841,572 + hycG b2719 0.14 -0.2
2,841,581 - hycG b2719 0.15 -0.4
2,841,622 - hycG b2719 0.20 +0.2
2,841,645 - hycG b2719 0.23 +0.2
2,841,648 - hycG b2719 0.24 -0.2
2,841,713 + hycG b2719 0.32 +0.1
2,841,774 - hycG b2719 0.40 +0.1
2,841,821 - hycG b2719 0.46 -0.7
2,841,861 - hycG b2719 0.52 -0.3
2,841,870 - hycG b2719 0.53 +0.4
2,842,019 + hycG b2719 0.72 +0.4
2,842,047 - hycG b2719 0.76 +0.1

Or see this region's nucleotide sequence