Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metB and metL are separated by 2 nucleotides metL and metF are separated by 348 nucleotides
b3939: metB - cystathionine gamma-synthase (NCBI), at 4,126,695 to 4,127,855
metB
b3940: metL - bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI), at 4,127,858 to 4,130,290
metL
b3941: metF - 5,10-methylenetetrahydrofolate reductase (NCBI), at 4,130,639 to 4,131,529
metF
Position (kb)
4127
4128
4129
4130
4131 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4126.900 kb on + strand, within metB at 4126.900 kb on + strand, within metB at 4126.902 kb on - strand, within metB at 4126.908 kb on - strand, within metB at 4126.910 kb on + strand, within metB at 4126.913 kb on - strand, within metB at 4127.015 kb on + strand, within metB at 4127.023 kb on + strand, within metB at 4127.042 kb on + strand, within metB at 4127.042 kb on + strand, within metB at 4127.112 kb on - strand, within metB at 4127.120 kb on + strand, within metB at 4127.152 kb on - strand, within metB at 4127.203 kb on - strand, within metB at 4127.232 kb on + strand, within metB at 4127.273 kb on + strand, within metB at 4127.273 kb on + strand, within metB at 4127.373 kb on - strand, within metB at 4127.400 kb on + strand, within metB at 4127.400 kb on + strand, within metB at 4127.410 kb on + strand, within metB at 4127.453 kb on - strand, within metB at 4127.592 kb on - strand, within metB at 4127.604 kb on - strand, within metB at 4127.663 kb on + strand, within metB at 4127.663 kb on + strand, within metB at 4127.709 kb on + strand, within metB at 4127.719 kb on + strand, within metB at 4127.720 kb on + strand, within metB at 4127.720 kb on + strand, within metB at 4127.725 kb on + strand, within metB at 4127.737 kb on - strand, within metB at 4127.737 kb on - strand, within metB at 4127.788 kb on + strand at 4127.796 kb on - strand at 4127.796 kb on - strand at 4127.796 kb on - strand at 4127.926 kb on - strand at 4127.978 kb on - strand at 4128.062 kb on - strand at 4128.238 kb on - strand, within metL at 4128.370 kb on + strand, within metL at 4128.487 kb on + strand, within metL at 4129.055 kb on + strand, within metL at 4129.114 kb on - strand, within metL at 4129.155 kb on + strand, within metL at 4129.197 kb on - strand, within metL at 4129.201 kb on + strand, within metL at 4129.236 kb on + strand, within metL at 4129.236 kb on + strand, within metL at 4129.236 kb on + strand, within metL at 4129.243 kb on - strand, within metL at 4129.244 kb on - strand, within metL at 4129.283 kb on - strand, within metL at 4129.290 kb on + strand, within metL at 4129.317 kb on + strand, within metL at 4129.332 kb on - strand, within metL at 4129.367 kb on + strand, within metL at 4129.444 kb on + strand, within metL at 4129.444 kb on + strand, within metL at 4129.444 kb on - strand, within metL at 4129.444 kb on - strand, within metL at 4129.452 kb on - strand, within metL at 4129.472 kb on - strand, within metL at 4129.472 kb on - strand, within metL at 4129.498 kb on - strand, within metL at 4129.596 kb on + strand, within metL at 4129.607 kb on + strand, within metL at 4129.607 kb on + strand, within metL at 4129.641 kb on + strand, within metL at 4129.641 kb on + strand, within metL at 4129.649 kb on - strand, within metL at 4129.649 kb on - strand, within metL at 4129.656 kb on - strand, within metL at 4129.684 kb on + strand, within metL at 4129.684 kb on + strand, within metL at 4129.684 kb on + strand, within metL at 4129.690 kb on - strand, within metL at 4129.690 kb on - strand, within metL at 4129.722 kb on + strand, within metL at 4129.759 kb on + strand, within metL at 4129.759 kb on + strand, within metL at 4129.759 kb on + strand, within metL at 4129.759 kb on + strand, within metL at 4129.783 kb on + strand, within metL at 4129.805 kb on + strand, within metL at 4129.811 kb on + strand, within metL at 4129.830 kb on + strand, within metL at 4129.836 kb on - strand, within metL at 4129.866 kb on + strand, within metL at 4129.866 kb on + strand, within metL at 4129.879 kb on + strand, within metL at 4129.879 kb on + strand, within metL at 4129.925 kb on - strand, within metL at 4129.928 kb on + strand, within metL at 4129.928 kb on + strand, within metL at 4129.938 kb on + strand, within metL at 4129.938 kb on + strand, within metL at 4129.953 kb on + strand, within metL at 4130.020 kb on - strand, within metL at 4130.079 kb on + strand at 4130.097 kb on - strand at 4130.097 kb on - strand at 4130.116 kb on - strand at 4130.194 kb on + strand at 4130.196 kb on - strand at 4130.202 kb on - strand at 4130.202 kb on - strand at 4130.211 kb on + strand at 4130.219 kb on + strand at 4130.229 kb on + strand at 4130.284 kb on - strand at 4130.309 kb on + strand at 4130.314 kb on + strand at 4130.325 kb on - strand at 4130.330 kb on - strand at 4130.378 kb on + strand at 4130.378 kb on + strand at 4130.386 kb on - strand at 4130.416 kb on + strand at 4130.479 kb on - strand at 4130.523 kb on - strand at 4130.609 kb on - strand at 4130.609 kb on - strand at 4130.614 kb on - strand at 4130.620 kb on - strand at 4130.631 kb on + strand at 4130.631 kb on + strand at 4130.678 kb on - strand at 4130.690 kb on - strand at 4130.787 kb on + strand, within metF at 4130.787 kb on + strand, within metF at 4130.795 kb on - strand, within metF at 4130.849 kb on - strand, within metF at 4130.853 kb on + strand, within metF at 4130.853 kb on + strand, within metF at 4130.856 kb on + strand, within metF at 4130.861 kb on - strand, within metF at 4130.882 kb on + strand, within metF at 4130.883 kb on + strand, within metF at 4130.885 kb on - strand, within metF at 4130.885 kb on - strand, within metF at 4130.940 kb on + strand, within metF at 4131.034 kb on - strand, within metF at 4131.079 kb on + strand, within metF at 4131.236 kb on - strand, within metF at 4131.286 kb on - strand, within metF
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 4,126,900 + metB b3939 0.18 +0.7 4,126,900 + metB b3939 0.18 +0.2 4,126,902 - metB b3939 0.18 -0.2 4,126,908 - metB b3939 0.18 +0.9 4,126,910 + metB b3939 0.19 -1.0 4,126,913 - metB b3939 0.19 +0.4 4,127,015 + metB b3939 0.28 +0.2 4,127,023 + metB b3939 0.28 +1.2 4,127,042 + metB b3939 0.30 +0.8 4,127,042 + metB b3939 0.30 +0.3 4,127,112 - metB b3939 0.36 +0.2 4,127,120 + metB b3939 0.37 +0.3 4,127,152 - metB b3939 0.39 -2.0 4,127,203 - metB b3939 0.44 -0.3 4,127,232 + metB b3939 0.46 -0.6 4,127,273 + metB b3939 0.50 -1.3 4,127,273 + metB b3939 0.50 -0.1 4,127,373 - metB b3939 0.58 -0.5 4,127,400 + metB b3939 0.61 +0.5 4,127,400 + metB b3939 0.61 +0.4 4,127,410 + metB b3939 0.62 -0.4 4,127,453 - metB b3939 0.65 -0.5 4,127,592 - metB b3939 0.77 +0.6 4,127,604 - metB b3939 0.78 -1.7 4,127,663 + metB b3939 0.83 -2.0 4,127,663 + metB b3939 0.83 -0.4 4,127,709 + metB b3939 0.87 +1.0 4,127,719 + metB b3939 0.88 +1.0 4,127,720 + metB b3939 0.88 -1.1 4,127,720 + metB b3939 0.88 -0.7 4,127,725 + metB b3939 0.89 +0.2 4,127,737 - metB b3939 0.90 +0.5 4,127,737 - metB b3939 0.90 -1.1 4,127,788 + -0.4 4,127,796 - +1.1 4,127,796 - +0.0 4,127,796 - -0.5 4,127,926 - -2.0 4,127,978 - -2.1 4,128,062 - -2.1 4,128,238 - metL b3940 0.16 -2.9 4,128,370 + metL b3940 0.21 -1.3 4,128,487 + metL b3940 0.26 +1.0 4,129,055 + metL b3940 0.49 -2.0 4,129,114 - metL b3940 0.52 -2.4 4,129,155 + metL b3940 0.53 +1.0 4,129,197 - metL b3940 0.55 -1.0 4,129,201 + metL b3940 0.55 +0.3 4,129,236 + metL b3940 0.57 +0.3 4,129,236 + metL b3940 0.57 -0.1 4,129,236 + metL b3940 0.57 -0.0 4,129,243 - metL b3940 0.57 -2.5 4,129,244 - metL b3940 0.57 -0.3 4,129,283 - metL b3940 0.59 -1.5 4,129,290 + metL b3940 0.59 -2.1 4,129,317 + metL b3940 0.60 -2.1 4,129,332 - metL b3940 0.61 -2.9 4,129,367 + metL b3940 0.62 -2.7 4,129,444 + metL b3940 0.65 -2.5 4,129,444 + metL b3940 0.65 -3.1 4,129,444 - metL b3940 0.65 -1.3 4,129,444 - metL b3940 0.65 -1.9 4,129,452 - metL b3940 0.66 -1.0 4,129,472 - metL b3940 0.66 -1.3 4,129,472 - metL b3940 0.66 +0.0 4,129,498 - metL b3940 0.67 -1.0 4,129,596 + metL b3940 0.71 -0.5 4,129,607 + metL b3940 0.72 -0.9 4,129,607 + metL b3940 0.72 +0.2 4,129,641 + metL b3940 0.73 -0.4 4,129,641 + metL b3940 0.73 +1.2 4,129,649 - metL b3940 0.74 -1.4 4,129,649 - metL b3940 0.74 -1.5 4,129,656 - metL b3940 0.74 -2.2 4,129,684 + metL b3940 0.75 -2.5 4,129,684 + metL b3940 0.75 -0.8 4,129,684 + metL b3940 0.75 -0.5 4,129,690 - metL b3940 0.75 -0.9 4,129,690 - metL b3940 0.75 -2.0 4,129,722 + metL b3940 0.77 -0.5 4,129,759 + metL b3940 0.78 +0.0 4,129,759 + metL b3940 0.78 -1.1 4,129,759 + metL b3940 0.78 -0.5 4,129,759 + metL b3940 0.78 +0.0 4,129,783 + metL b3940 0.79 -1.8 4,129,805 + metL b3940 0.80 -0.8 4,129,811 + metL b3940 0.80 -0.0 4,129,830 + metL b3940 0.81 -0.2 4,129,836 - metL b3940 0.81 +0.2 4,129,866 + metL b3940 0.83 +0.1 4,129,866 + metL b3940 0.83 +0.3 4,129,879 + metL b3940 0.83 -2.2 4,129,879 + metL b3940 0.83 +0.0 4,129,925 - metL b3940 0.85 -2.8 4,129,928 + metL b3940 0.85 +1.0 4,129,928 + metL b3940 0.85 +0.7 4,129,938 + metL b3940 0.85 -0.9 4,129,938 + metL b3940 0.85 -0.7 4,129,953 + metL b3940 0.86 -1.6 4,130,020 - metL b3940 0.89 -1.7 4,130,079 + +0.1 4,130,097 - +0.7 4,130,097 - +1.0 4,130,116 - -1.4 4,130,194 + -0.8 4,130,196 - -2.2 4,130,202 - +0.7 4,130,202 - -3.3 4,130,211 + +0.2 4,130,219 + -0.1 4,130,229 + +0.4 4,130,284 - -1.1 4,130,309 + -0.4 4,130,314 + -0.1 4,130,325 - +0.1 4,130,330 - +0.3 4,130,378 + +0.3 4,130,378 + +0.6 4,130,386 - +1.5 4,130,416 + -0.1 4,130,479 - +1.1 4,130,523 - +0.8 4,130,609 - -0.2 4,130,609 - +0.7 4,130,614 - -0.4 4,130,620 - +0.4 4,130,631 + +0.2 4,130,631 + +0.1 4,130,678 - +0.3 4,130,690 - -0.2 4,130,787 + metF b3941 0.17 -1.3 4,130,787 + metF b3941 0.17 -0.5 4,130,795 - metF b3941 0.18 -0.0 4,130,849 - metF b3941 0.24 +0.0 4,130,853 + metF b3941 0.24 -0.1 4,130,853 + metF b3941 0.24 -0.3 4,130,856 + metF b3941 0.24 -0.2 4,130,861 - metF b3941 0.25 +0.1 4,130,882 + metF b3941 0.27 +0.7 4,130,883 + metF b3941 0.27 +0.3 4,130,885 - metF b3941 0.28 +0.7 4,130,885 - metF b3941 0.28 +1.3 4,130,940 + metF b3941 0.34 +0.1 4,131,034 - metF b3941 0.44 +0.2 4,131,079 + metF b3941 0.49 +0.1 4,131,236 - metF b3941 0.67 -0.6 4,131,286 - metF b3941 0.73 +0.5
Or see this region's nucleotide sequence