Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt htrL and rfaD are separated by 303 nucleotides rfaD and rfaF are separated by 9 nucleotides
b3618: htrL - involved in lipopolysaccharide biosynthesis (VIMSS), at 3,790,849 to 3,791,706
htrL
b3619: rfaD - ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI), at 3,792,010 to 3,792,942
rfaD
b3620: rfaF - ADP-heptose:LPS heptosyltransferase II (NCBI), at 3,792,952 to 3,793,998
rfaF
Position (kb)
3792
3793 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3791.016 kb on - strand, within htrL at 3791.016 kb on - strand, within htrL at 3791.156 kb on - strand, within htrL at 3791.156 kb on - strand, within htrL at 3791.164 kb on - strand, within htrL at 3791.183 kb on - strand, within htrL at 3791.191 kb on - strand, within htrL at 3791.191 kb on - strand, within htrL at 3791.193 kb on - strand, within htrL at 3791.203 kb on - strand, within htrL at 3791.246 kb on - strand, within htrL at 3791.247 kb on - strand, within htrL at 3791.270 kb on - strand, within htrL at 3791.271 kb on - strand, within htrL at 3791.304 kb on - strand, within htrL at 3791.304 kb on - strand, within htrL at 3791.304 kb on - strand, within htrL at 3791.316 kb on - strand, within htrL at 3791.316 kb on - strand, within htrL at 3791.347 kb on - strand, within htrL at 3791.359 kb on - strand, within htrL at 3791.360 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.361 kb on - strand, within htrL at 3791.419 kb on - strand, within htrL at 3791.571 kb on - strand, within htrL at 3791.571 kb on - strand, within htrL at 3791.693 kb on - strand at 3791.774 kb on - strand at 3791.774 kb on - strand at 3791.823 kb on + strand at 3791.841 kb on - strand at 3791.851 kb on - strand at 3791.851 kb on - strand at 3791.856 kb on + strand at 3791.857 kb on + strand at 3791.857 kb on + strand at 3791.859 kb on + strand at 3791.859 kb on + strand at 3791.864 kb on + strand at 3791.864 kb on - strand at 3791.864 kb on - strand at 3791.864 kb on - strand at 3791.877 kb on - strand at 3791.877 kb on - strand at 3791.877 kb on - strand at 3791.877 kb on - strand at 3791.896 kb on - strand at 3791.896 kb on - strand at 3791.898 kb on - strand at 3791.904 kb on - strand at 3791.913 kb on + strand at 3791.913 kb on + strand at 3791.914 kb on - strand at 3791.914 kb on - strand at 3791.919 kb on - strand at 3791.919 kb on - strand at 3791.919 kb on - strand at 3791.919 kb on - strand at 3791.919 kb on - strand at 3791.921 kb on - strand at 3791.922 kb on - strand at 3791.924 kb on - strand at 3791.924 kb on - strand at 3791.928 kb on + strand at 3791.931 kb on - strand at 3791.932 kb on - strand at 3791.952 kb on - strand at 3791.955 kb on - strand at 3791.959 kb on - strand at 3791.967 kb on + strand at 3791.974 kb on + strand at 3792.061 kb on - strand at 3792.061 kb on - strand at 3792.113 kb on - strand, within rfaD at 3792.113 kb on - strand, within rfaD at 3792.152 kb on - strand, within rfaD at 3792.270 kb on + strand, within rfaD at 3792.370 kb on + strand, within rfaD at 3792.380 kb on + strand, within rfaD at 3792.380 kb on + strand, within rfaD at 3792.389 kb on + strand, within rfaD at 3792.397 kb on - strand, within rfaD at 3792.462 kb on + strand, within rfaD at 3792.464 kb on + strand, within rfaD at 3792.479 kb on + strand, within rfaD at 3792.546 kb on - strand, within rfaD at 3792.588 kb on - strand, within rfaD at 3792.588 kb on - strand, within rfaD at 3792.661 kb on + strand, within rfaD at 3792.662 kb on - strand, within rfaD at 3792.670 kb on + strand, within rfaD at 3792.683 kb on - strand, within rfaD at 3792.705 kb on + strand, within rfaD at 3792.713 kb on - strand, within rfaD at 3792.714 kb on - strand, within rfaD at 3792.726 kb on + strand, within rfaD at 3792.730 kb on - strand, within rfaD at 3792.730 kb on - strand, within rfaD at 3792.736 kb on + strand, within rfaD at 3792.755 kb on - strand, within rfaD at 3792.771 kb on + strand, within rfaD at 3792.907 kb on + strand at 3792.915 kb on - strand at 3792.915 kb on - strand at 3792.946 kb on - strand at 3792.975 kb on + strand at 3792.975 kb on + strand at 3792.975 kb on + strand at 3793.011 kb on + strand at 3793.021 kb on + strand at 3793.021 kb on + strand at 3793.021 kb on + strand at 3793.034 kb on + strand at 3793.124 kb on - strand, within rfaF at 3793.169 kb on + strand, within rfaF at 3793.234 kb on - strand, within rfaF at 3793.259 kb on - strand, within rfaF at 3793.305 kb on - strand, within rfaF at 3793.307 kb on + strand, within rfaF at 3793.307 kb on + strand, within rfaF at 3793.307 kb on + strand, within rfaF at 3793.307 kb on - strand, within rfaF at 3793.311 kb on + strand, within rfaF at 3793.328 kb on - strand, within rfaF at 3793.350 kb on + strand at 3793.353 kb on + strand, within rfaF at 3793.358 kb on - strand, within rfaF at 3793.358 kb on - strand, within rfaF at 3793.393 kb on + strand, within rfaF at 3793.393 kb on + strand, within rfaF at 3793.393 kb on + strand, within rfaF at 3793.513 kb on + strand, within rfaF at 3793.543 kb on + strand, within rfaF at 3793.546 kb on + strand, within rfaF at 3793.554 kb on - strand, within rfaF at 3793.554 kb on - strand, within rfaF at 3793.589 kb on + strand, within rfaF at 3793.589 kb on + strand, within rfaF at 3793.595 kb on - strand, within rfaF at 3793.600 kb on + strand, within rfaF at 3793.663 kb on + strand, within rfaF at 3793.725 kb on - strand, within rfaF at 3793.725 kb on - strand, within rfaF at 3793.734 kb on - strand, within rfaF at 3793.734 kb on - strand, within rfaF at 3793.765 kb on - strand, within rfaF at 3793.775 kb on + strand, within rfaF at 3793.775 kb on + strand, within rfaF at 3793.813 kb on + strand, within rfaF at 3793.813 kb on + strand at 3793.821 kb on - strand, within rfaF at 3793.857 kb on - strand, within rfaF at 3793.857 kb on - strand, within rfaF at 3793.916 kb on + strand at 3793.921 kb on - strand at 3793.921 kb on - strand at 3793.926 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 3,791,016 - htrL b3618 0.19 +0.4 3,791,016 - htrL b3618 0.19 -0.7 3,791,156 - htrL b3618 0.36 +2.0 3,791,156 - htrL b3618 0.36 +1.0 3,791,164 - htrL b3618 0.37 -2.2 3,791,183 - htrL b3618 0.39 +1.4 3,791,191 - htrL b3618 0.40 +0.3 3,791,191 - htrL b3618 0.40 +0.7 3,791,193 - htrL b3618 0.40 -1.8 3,791,203 - htrL b3618 0.41 +1.4 3,791,246 - htrL b3618 0.46 -1.3 3,791,247 - htrL b3618 0.46 +1.0 3,791,270 - htrL b3618 0.49 +1.0 3,791,271 - htrL b3618 0.49 -1.4 3,791,304 - htrL b3618 0.53 +1.0 3,791,304 - htrL b3618 0.53 +1.0 3,791,304 - htrL b3618 0.53 +0.0 3,791,316 - htrL b3618 0.54 +1.0 3,791,316 - htrL b3618 0.54 -1.3 3,791,347 - htrL b3618 0.58 -2.2 3,791,359 - htrL b3618 0.59 +1.0 3,791,360 - htrL b3618 0.60 +1.0 3,791,361 - htrL b3618 0.60 -1.1 3,791,361 - htrL b3618 0.60 +2.6 3,791,361 - htrL b3618 0.60 +2.0 3,791,361 - htrL b3618 0.60 +2.6 3,791,361 - htrL b3618 0.60 +2.0 3,791,419 - htrL b3618 0.66 +0.0 3,791,571 - htrL b3618 0.84 +1.0 3,791,571 - htrL b3618 0.84 +1.0 3,791,693 - +1.0 3,791,774 - -0.3 3,791,774 - -0.8 3,791,823 + +1.0 3,791,841 - +0.2 3,791,851 - -2.1 3,791,851 - -0.7 3,791,856 + -0.7 3,791,857 + -0.6 3,791,857 + -1.7 3,791,859 + -1.3 3,791,859 + +0.1 3,791,864 + -0.4 3,791,864 - +0.2 3,791,864 - -0.8 3,791,864 - -0.4 3,791,877 - -1.2 3,791,877 - +0.2 3,791,877 - -1.6 3,791,877 - -0.3 3,791,896 - -0.1 3,791,896 - -0.3 3,791,898 - +0.9 3,791,904 - +0.1 3,791,913 + -3.0 3,791,913 + -1.3 3,791,914 - -0.2 3,791,914 - -0.7 3,791,919 - +0.6 3,791,919 - +1.0 3,791,919 - +1.1 3,791,919 - -0.5 3,791,919 - +0.1 3,791,921 - +0.4 3,791,922 - -0.4 3,791,924 - +0.2 3,791,924 - -0.9 3,791,928 + +1.3 3,791,931 - -0.8 3,791,932 - -0.3 3,791,952 - -1.2 3,791,955 - -1.3 3,791,959 - +0.7 3,791,967 + -1.2 3,791,974 + -1.4 3,792,061 - +1.0 3,792,061 - +2.6 3,792,113 - rfaD b3619 0.11 -1.6 3,792,113 - rfaD b3619 0.11 -2.6 3,792,152 - rfaD b3619 0.15 +2.6 3,792,270 + rfaD b3619 0.28 -2.3 3,792,370 + rfaD b3619 0.39 -2.3 3,792,380 + rfaD b3619 0.40 -2.3 3,792,380 + rfaD b3619 0.40 -0.4 3,792,389 + rfaD b3619 0.41 +0.0 3,792,397 - rfaD b3619 0.41 +0.0 3,792,462 + rfaD b3619 0.48 -2.7 3,792,464 + rfaD b3619 0.49 +0.0 3,792,479 + rfaD b3619 0.50 -0.7 3,792,546 - rfaD b3619 0.57 -1.6 3,792,588 - rfaD b3619 0.62 +1.0 3,792,588 - rfaD b3619 0.62 +1.0 3,792,661 + rfaD b3619 0.70 -1.8 3,792,662 - rfaD b3619 0.70 -1.9 3,792,670 + rfaD b3619 0.71 -2.7 3,792,683 - rfaD b3619 0.72 -2.2 3,792,705 + rfaD b3619 0.74 -1.3 3,792,713 - rfaD b3619 0.75 -0.6 3,792,714 - rfaD b3619 0.75 -1.8 3,792,726 + rfaD b3619 0.77 +1.0 3,792,730 - rfaD b3619 0.77 -1.0 3,792,730 - rfaD b3619 0.77 -1.0 3,792,736 + rfaD b3619 0.78 -2.5 3,792,755 - rfaD b3619 0.80 +0.0 3,792,771 + rfaD b3619 0.82 -3.2 3,792,907 + +1.0 3,792,915 - +0.0 3,792,915 - +1.0 3,792,946 - -4.5 3,792,975 + +0.0 3,792,975 + -1.3 3,792,975 + -1.2 3,793,011 + -0.8 3,793,021 + +1.0 3,793,021 + +1.0 3,793,021 + +1.0 3,793,034 + -2.0 3,793,124 - rfaF b3620 0.16 +0.4 3,793,169 + rfaF b3620 0.21 +0.7 3,793,234 - rfaF b3620 0.27 -0.9 3,793,259 - rfaF b3620 0.29 -3.5 3,793,305 - rfaF b3620 0.34 -2.0 3,793,307 + rfaF b3620 0.34 +0.0 3,793,307 + rfaF b3620 0.34 +1.0 3,793,307 + rfaF b3620 0.34 +1.0 3,793,307 - rfaF b3620 0.34 -1.0 3,793,311 + rfaF b3620 0.34 -1.8 3,793,328 - rfaF b3620 0.36 +2.6 3,793,350 + -1.3 3,793,353 + rfaF b3620 0.38 -2.2 3,793,358 - rfaF b3620 0.39 +0.3 3,793,358 - rfaF b3620 0.39 -2.9 3,793,393 + rfaF b3620 0.42 -1.3 3,793,393 + rfaF b3620 0.42 -0.6 3,793,393 + rfaF b3620 0.42 -1.0 3,793,513 + rfaF b3620 0.54 -1.8 3,793,543 + rfaF b3620 0.56 -0.9 3,793,546 + rfaF b3620 0.57 -2.7 3,793,554 - rfaF b3620 0.57 -1.3 3,793,554 - rfaF b3620 0.57 -1.3 3,793,589 + rfaF b3620 0.61 -2.8 3,793,589 + rfaF b3620 0.61 -1.6 3,793,595 - rfaF b3620 0.61 -0.3 3,793,600 + rfaF b3620 0.62 -1.3 3,793,663 + rfaF b3620 0.68 -1.8 3,793,725 - rfaF b3620 0.74 +0.0 3,793,725 - rfaF b3620 0.74 -2.0 3,793,734 - rfaF b3620 0.75 -0.8 3,793,734 - rfaF b3620 0.75 -2.6 3,793,765 - rfaF b3620 0.78 -1.9 3,793,775 + rfaF b3620 0.79 -0.2 3,793,775 + rfaF b3620 0.79 -2.2 3,793,813 + rfaF b3620 0.82 +0.0 3,793,813 + +3.0 3,793,821 - rfaF b3620 0.83 -1.3 3,793,857 - rfaF b3620 0.86 -1.0 3,793,857 - rfaF b3620 0.86 -2.6 3,793,916 + +0.0 3,793,921 - +2.0 3,793,921 - +0.4 3,793,926 - +0.0
Or see this region's nucleotide sequence