Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt envZ and ompR overlap by 4 nucleotides ompR and greB are separated by 227 nucleotides greB and yhgF are separated by 96 nucleotides
b3404: envZ - osmolarity sensor protein (NCBI), at 3,532,538 to 3,533,890
envZ
b3405: ompR - osmolarity response regulator (NCBI), at 3,533,887 to 3,534,606
ompR
b3406: greB - transcription elongation factor GreB (NCBI), at 3,534,834 to 3,535,310
greB
b3407: yhgF - orf, hypothetical protein (VIMSS), at 3,535,407 to 3,537,728
yhgF
Position (kb)
3533
3534
3535 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3532.888 kb on - strand, within envZ at 3532.919 kb on + strand, within envZ at 3532.970 kb on - strand, within envZ at 3532.973 kb on + strand, within envZ at 3533.031 kb on - strand, within envZ at 3533.093 kb on + strand, within envZ at 3533.139 kb on - strand, within envZ at 3533.159 kb on + strand, within envZ at 3533.203 kb on + strand, within envZ at 3533.264 kb on + strand, within envZ at 3533.306 kb on + strand, within envZ at 3533.314 kb on - strand, within envZ at 3533.326 kb on - strand, within envZ at 3533.342 kb on + strand, within envZ at 3533.350 kb on - strand, within envZ at 3533.350 kb on - strand, within envZ at 3533.360 kb on - strand, within envZ at 3533.360 kb on - strand, within envZ at 3533.398 kb on + strand, within envZ at 3533.400 kb on + strand, within envZ at 3533.406 kb on - strand, within envZ at 3533.458 kb on + strand, within envZ at 3533.460 kb on + strand, within envZ at 3533.492 kb on + strand, within envZ at 3533.519 kb on + strand, within envZ at 3533.523 kb on - strand, within envZ at 3533.571 kb on - strand, within envZ at 3533.571 kb on - strand, within envZ at 3533.572 kb on - strand, within envZ at 3533.599 kb on - strand, within envZ at 3533.629 kb on - strand, within envZ at 3533.676 kb on + strand, within envZ at 3533.693 kb on + strand, within envZ at 3533.723 kb on + strand, within envZ at 3533.832 kb on + strand at 3533.853 kb on - strand at 3533.853 kb on - strand at 3533.871 kb on - strand at 3533.892 kb on + strand at 3533.900 kb on - strand at 3533.900 kb on - strand at 3533.900 kb on - strand at 3533.900 kb on - strand at 3533.907 kb on + strand at 3533.926 kb on + strand at 3533.933 kb on - strand at 3533.950 kb on - strand at 3534.010 kb on - strand, within ompR at 3534.010 kb on - strand, within ompR at 3534.057 kb on - strand, within ompR at 3534.093 kb on + strand, within ompR at 3534.097 kb on + strand, within ompR at 3534.097 kb on + strand, within ompR at 3534.097 kb on + strand, within ompR at 3534.105 kb on - strand, within ompR at 3534.105 kb on - strand, within ompR at 3534.129 kb on - strand, within ompR at 3534.168 kb on - strand, within ompR at 3534.206 kb on + strand, within ompR at 3534.228 kb on - strand, within ompR at 3534.257 kb on - strand, within ompR at 3534.260 kb on - strand, within ompR at 3534.267 kb on - strand, within ompR at 3534.267 kb on - strand, within ompR at 3534.358 kb on - strand, within ompR at 3534.436 kb on + strand, within ompR at 3534.447 kb on + strand, within ompR at 3534.456 kb on - strand, within ompR at 3534.509 kb on + strand, within ompR at 3534.514 kb on + strand, within ompR at 3534.514 kb on + strand, within ompR at 3534.522 kb on - strand, within ompR at 3534.541 kb on - strand at 3534.541 kb on - strand at 3534.544 kb on + strand at 3534.606 kb on - strand at 3534.608 kb on + strand at 3534.608 kb on + strand at 3534.616 kb on + strand at 3534.625 kb on - strand at 3534.632 kb on + strand at 3534.684 kb on - strand at 3534.724 kb on - strand at 3534.783 kb on - strand at 3534.851 kb on - strand at 3534.851 kb on - strand at 3534.852 kb on + strand at 3534.861 kb on - strand at 3534.861 kb on - strand at 3534.889 kb on + strand, within greB at 3534.889 kb on + strand, within greB at 3534.940 kb on - strand, within greB at 3534.940 kb on - strand, within greB at 3534.988 kb on - strand, within greB at 3535.024 kb on + strand, within greB at 3535.027 kb on - strand, within greB at 3535.027 kb on - strand, within greB at 3535.030 kb on - strand, within greB at 3535.066 kb on - strand, within greB at 3535.094 kb on - strand, within greB at 3535.099 kb on + strand, within greB at 3535.149 kb on + strand, within greB at 3535.257 kb on - strand, within greB at 3535.257 kb on - strand, within greB at 3535.274 kb on + strand at 3535.287 kb on + strand at 3535.297 kb on + strand at 3535.297 kb on + strand at 3535.299 kb on + strand at 3535.305 kb on - strand at 3535.305 kb on - strand at 3535.317 kb on + strand at 3535.317 kb on - strand at 3535.325 kb on - strand at 3535.325 kb on - strand at 3535.359 kb on + strand at 3535.359 kb on + strand at 3535.361 kb on + strand at 3535.397 kb on + strand at 3535.485 kb on - strand at 3535.496 kb on + strand at 3535.496 kb on + strand at 3535.498 kb on + strand at 3535.546 kb on + strand at 3535.563 kb on - strand at 3535.563 kb on - strand at 3535.573 kb on - strand at 3535.581 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 3,532,888 - envZ b3404 0.26 -1.1 3,532,919 + envZ b3404 0.28 -1.0 3,532,970 - envZ b3404 0.32 -2.0 3,532,973 + envZ b3404 0.32 -3.7 3,533,031 - envZ b3404 0.36 +0.4 3,533,093 + envZ b3404 0.41 -0.6 3,533,139 - envZ b3404 0.44 -2.0 3,533,159 + envZ b3404 0.46 -2.7 3,533,203 + envZ b3404 0.49 -1.0 3,533,264 + envZ b3404 0.54 -1.0 3,533,306 + envZ b3404 0.57 -0.6 3,533,314 - envZ b3404 0.57 +0.3 3,533,326 - envZ b3404 0.58 -0.4 3,533,342 + envZ b3404 0.59 -3.7 3,533,350 - envZ b3404 0.60 -0.5 3,533,350 - envZ b3404 0.60 +1.5 3,533,360 - envZ b3404 0.61 -1.3 3,533,360 - envZ b3404 0.61 -0.8 3,533,398 + envZ b3404 0.64 -1.4 3,533,400 + envZ b3404 0.64 -3.1 3,533,406 - envZ b3404 0.64 -0.9 3,533,458 + envZ b3404 0.68 -1.3 3,533,460 + envZ b3404 0.68 -0.6 3,533,492 + envZ b3404 0.71 -3.1 3,533,519 + envZ b3404 0.73 -0.8 3,533,523 - envZ b3404 0.73 -2.1 3,533,571 - envZ b3404 0.76 -1.1 3,533,571 - envZ b3404 0.76 -1.9 3,533,572 - envZ b3404 0.76 -0.8 3,533,599 - envZ b3404 0.78 -1.6 3,533,629 - envZ b3404 0.81 -0.8 3,533,676 + envZ b3404 0.84 -0.7 3,533,693 + envZ b3404 0.85 -2.0 3,533,723 + envZ b3404 0.88 -1.2 3,533,832 + -1.2 3,533,853 - -2.9 3,533,853 - -2.0 3,533,871 - -1.1 3,533,892 + -2.7 3,533,900 - +0.7 3,533,900 - +0.0 3,533,900 - +2.1 3,533,900 - -0.3 3,533,907 + -2.9 3,533,926 + -1.3 3,533,933 - -1.6 3,533,950 - -0.6 3,534,010 - ompR b3405 0.17 -1.0 3,534,010 - ompR b3405 0.17 -2.2 3,534,057 - ompR b3405 0.24 -0.0 3,534,093 + ompR b3405 0.29 -1.7 3,534,097 + ompR b3405 0.29 -2.9 3,534,097 + ompR b3405 0.29 +1.0 3,534,097 + ompR b3405 0.29 -3.2 3,534,105 - ompR b3405 0.30 -2.2 3,534,105 - ompR b3405 0.30 -1.6 3,534,129 - ompR b3405 0.34 -3.8 3,534,168 - ompR b3405 0.39 -3.0 3,534,206 + ompR b3405 0.44 -0.6 3,534,228 - ompR b3405 0.47 -2.9 3,534,257 - ompR b3405 0.51 -2.3 3,534,260 - ompR b3405 0.52 -1.0 3,534,267 - ompR b3405 0.53 -0.6 3,534,267 - ompR b3405 0.53 -1.3 3,534,358 - ompR b3405 0.65 +0.1 3,534,436 + ompR b3405 0.76 +1.0 3,534,447 + ompR b3405 0.78 +2.0 3,534,456 - ompR b3405 0.79 -2.5 3,534,509 + ompR b3405 0.86 -2.2 3,534,514 + ompR b3405 0.87 -2.0 3,534,514 + ompR b3405 0.87 -1.8 3,534,522 - ompR b3405 0.88 -2.9 3,534,541 - -1.6 3,534,541 - -1.0 3,534,544 + -1.6 3,534,606 - -0.9 3,534,608 + +0.3 3,534,608 + -1.5 3,534,616 + +0.4 3,534,625 - -1.3 3,534,632 + +1.3 3,534,684 - +0.3 3,534,724 - +0.2 3,534,783 - +0.6 3,534,851 - -1.0 3,534,851 - -1.1 3,534,852 + -1.3 3,534,861 - +1.2 3,534,861 - +0.6 3,534,889 + greB b3406 0.12 -0.7 3,534,889 + greB b3406 0.12 -1.1 3,534,940 - greB b3406 0.22 -1.9 3,534,940 - greB b3406 0.22 -0.1 3,534,988 - greB b3406 0.32 +0.2 3,535,024 + greB b3406 0.40 +0.4 3,535,027 - greB b3406 0.40 -1.7 3,535,027 - greB b3406 0.40 -0.2 3,535,030 - greB b3406 0.41 -0.1 3,535,066 - greB b3406 0.49 +0.6 3,535,094 - greB b3406 0.55 +0.1 3,535,099 + greB b3406 0.56 +0.4 3,535,149 + greB b3406 0.66 -0.5 3,535,257 - greB b3406 0.89 +0.3 3,535,257 - greB b3406 0.89 +0.2 3,535,274 + +0.4 3,535,287 + -0.1 3,535,297 + +0.1 3,535,297 + +0.7 3,535,299 + -0.4 3,535,305 - +0.4 3,535,305 - +1.4 3,535,317 + -0.1 3,535,317 - +0.0 3,535,325 - +1.0 3,535,325 - -0.5 3,535,359 + +0.0 3,535,359 + -1.0 3,535,361 + +0.6 3,535,397 + +0.4 3,535,485 - +0.1 3,535,496 + -0.2 3,535,496 + +2.0 3,535,498 + -0.7 3,535,546 + +0.6 3,535,563 - +1.0 3,535,563 - +0.5 3,535,573 - -0.2 3,535,581 + -1.7
Or see this region's nucleotide sequence