Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhdE and mreD are separated by 8 nucleotides mreD and mreC overlap by 1 nucleotides mreC and mreB are separated by 65 nucleotides
b3248: yhdE - Maf-like protein (NCBI), at 3,395,807 to 3,396,400
yhdE
b3249: mreD - cell wall structural complex MreBCD transmembrane component MreD (NCBI), at 3,396,409 to 3,396,897
mreD
b3250: mreC - cell wall structural complex MreBCD transmembrane component MreC (NCBI), at 3,396,897 to 3,398,000
mreC
b3251: mreB - regulator of ftsI, penicillin binding protein 3, septation function (VIMSS), at 3,398,066 to 3,399,109
mreB
Position (kb)
3396
3397
3398
3399 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3395.897 kb on - strand, within yhdE at 3395.897 kb on - strand, within yhdE at 3395.923 kb on + strand, within yhdE at 3395.923 kb on + strand, within yhdE at 3395.941 kb on + strand, within yhdE at 3395.956 kb on - strand, within yhdE at 3395.964 kb on - strand, within yhdE at 3395.966 kb on + strand, within yhdE at 3395.987 kb on - strand, within yhdE at 3395.987 kb on - strand, within yhdE at 3396.058 kb on - strand, within yhdE at 3396.117 kb on - strand, within yhdE at 3396.139 kb on + strand, within yhdE at 3396.166 kb on - strand, within yhdE at 3396.166 kb on - strand, within yhdE at 3396.176 kb on - strand, within yhdE at 3396.186 kb on - strand, within yhdE at 3396.192 kb on + strand, within yhdE at 3396.231 kb on + strand, within yhdE at 3396.231 kb on + strand, within yhdE at 3396.256 kb on + strand, within yhdE at 3396.267 kb on + strand, within yhdE at 3396.269 kb on + strand, within yhdE at 3396.275 kb on - strand, within yhdE at 3396.275 kb on - strand, within yhdE at 3396.287 kb on - strand, within yhdE at 3396.287 kb on - strand, within yhdE at 3396.307 kb on - strand, within yhdE at 3396.317 kb on - strand, within yhdE at 3396.333 kb on - strand, within yhdE at 3396.344 kb on - strand at 3396.344 kb on - strand at 3396.364 kb on - strand at 3396.364 kb on - strand at 3396.417 kb on - strand at 3396.924 kb on - strand at 3396.924 kb on - strand at 3396.934 kb on - strand at 3396.962 kb on - strand at 3397.084 kb on - strand, within mreC at 3397.174 kb on - strand, within mreC at 3397.973 kb on - strand at 3397.974 kb on - strand at 3397.991 kb on - strand at 3398.003 kb on - strand at 3398.031 kb on - strand at 3398.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 3,395,897 - yhdE b3248 0.15 +0.2 3,395,897 - yhdE b3248 0.15 +1.2 3,395,923 + yhdE b3248 0.20 -0.1 3,395,923 + yhdE b3248 0.20 +0.2 3,395,941 + yhdE b3248 0.23 +0.3 3,395,956 - yhdE b3248 0.25 +0.4 3,395,964 - yhdE b3248 0.26 -0.6 3,395,966 + yhdE b3248 0.27 -0.1 3,395,987 - yhdE b3248 0.30 -0.8 3,395,987 - yhdE b3248 0.30 +0.5 3,396,058 - yhdE b3248 0.42 +0.0 3,396,117 - yhdE b3248 0.52 -0.2 3,396,139 + yhdE b3248 0.56 -0.2 3,396,166 - yhdE b3248 0.60 -1.2 3,396,166 - yhdE b3248 0.60 +0.3 3,396,176 - yhdE b3248 0.62 +0.1 3,396,186 - yhdE b3248 0.64 +0.2 3,396,192 + yhdE b3248 0.65 +1.1 3,396,231 + yhdE b3248 0.71 +1.3 3,396,231 + yhdE b3248 0.71 +0.0 3,396,256 + yhdE b3248 0.76 +0.3 3,396,267 + yhdE b3248 0.77 -0.4 3,396,269 + yhdE b3248 0.78 -0.5 3,396,275 - yhdE b3248 0.79 -0.3 3,396,275 - yhdE b3248 0.79 +0.1 3,396,287 - yhdE b3248 0.81 -1.5 3,396,287 - yhdE b3248 0.81 +0.4 3,396,307 - yhdE b3248 0.84 +1.6 3,396,317 - yhdE b3248 0.86 -0.6 3,396,333 - yhdE b3248 0.89 +0.4 3,396,344 - -0.7 3,396,344 - -0.5 3,396,364 - -0.1 3,396,364 - -1.0 3,396,417 - -0.7 3,396,924 - +0.1 3,396,924 - +0.1 3,396,934 - -1.8 3,396,962 - +0.7 3,397,084 - mreC b3250 0.17 -0.7 3,397,174 - mreC b3250 0.25 -3.8 3,397,973 - +0.2 3,397,974 - -0.6 3,397,991 - +0.9 3,398,003 - +0.7 3,398,031 - -0.4 3,398,032 - +0.2
Or see this region's nucleotide sequence