Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadH and ygjM are separated by 44 nucleotides ygjM and ygjN overlap by 4 nucleotides ygjN and ygjO are separated by 283 nucleotides
b3081: fadH - 2,4-dienoyl-CoA reductase, NADH and FMN-linked (NCBI), at 3,229,687 to 3,231,705
fadH
b3082: ygjM - predicted DNA-binding transcriptional regulator (NCBI), at 3,231,750 to 3,232,166
ygjM
b3083: ygjN - hypothetical protein (NCBI), at 3,232,163 to 3,232,477
ygjN
b3084: ygjO - putative enzyme (VIMSS), at 3,232,761 to 3,233,897
ygjO
Position (kb)
3231
3232
3233 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3230.922 kb on + strand, within fadH at 3230.928 kb on - strand, within fadH at 3231.019 kb on + strand, within fadH at 3231.139 kb on + strand, within fadH at 3231.163 kb on - strand, within fadH at 3231.199 kb on - strand, within fadH at 3231.267 kb on + strand, within fadH at 3231.267 kb on + strand, within fadH at 3231.356 kb on + strand, within fadH at 3231.399 kb on - strand, within fadH at 3231.427 kb on - strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.504 kb on - strand at 3231.530 kb on + strand at 3231.530 kb on + strand at 3231.547 kb on + strand at 3231.552 kb on + strand at 3231.643 kb on + strand at 3231.673 kb on + strand at 3231.812 kb on - strand, within ygjM at 3231.855 kb on - strand, within ygjM at 3231.856 kb on - strand, within ygjM at 3231.892 kb on - strand, within ygjM at 3231.892 kb on - strand, within ygjM at 3232.179 kb on + strand at 3232.179 kb on + strand at 3232.181 kb on + strand at 3232.186 kb on + strand at 3232.189 kb on - strand at 3232.233 kb on + strand, within ygjN at 3232.267 kb on + strand, within ygjN at 3232.272 kb on - strand, within ygjN at 3232.302 kb on + strand, within ygjN at 3232.394 kb on + strand, within ygjN at 3232.416 kb on + strand, within ygjN at 3232.465 kb on - strand at 3232.465 kb on - strand at 3232.465 kb on - strand at 3232.471 kb on + strand at 3232.603 kb on + strand at 3232.603 kb on + strand at 3232.704 kb on - strand at 3232.704 kb on - strand at 3232.783 kb on + strand at 3232.783 kb on + strand at 3232.815 kb on + strand at 3232.830 kb on - strand at 3232.862 kb on + strand at 3232.862 kb on + strand at 3232.924 kb on - strand, within ygjO at 3232.967 kb on - strand, within ygjO at 3232.979 kb on - strand, within ygjO at 3232.979 kb on - strand, within ygjO at 3232.999 kb on - strand, within ygjO at 3232.999 kb on - strand, within ygjO
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 3,230,922 + fadH b3081 0.61 +0.7 3,230,928 - fadH b3081 0.61 +0.0 3,231,019 + fadH b3081 0.66 +1.1 3,231,139 + fadH b3081 0.72 +0.0 3,231,163 - fadH b3081 0.73 +0.1 3,231,199 - fadH b3081 0.75 -0.5 3,231,267 + fadH b3081 0.78 -0.9 3,231,267 + fadH b3081 0.78 -0.3 3,231,356 + fadH b3081 0.83 +0.6 3,231,399 - fadH b3081 0.85 +0.5 3,231,427 - fadH b3081 0.86 +0.5 3,231,428 + fadH b3081 0.86 +0.3 3,231,428 + fadH b3081 0.86 +0.3 3,231,428 + fadH b3081 0.86 -0.9 3,231,428 + fadH b3081 0.86 -0.6 3,231,504 - -0.3 3,231,530 + +0.2 3,231,530 + +0.2 3,231,547 + -0.1 3,231,552 + -0.9 3,231,643 + +0.9 3,231,673 + +0.9 3,231,812 - ygjM b3082 0.15 -1.6 3,231,855 - ygjM b3082 0.25 +1.0 3,231,856 - ygjM b3082 0.25 -2.3 3,231,892 - ygjM b3082 0.34 -1.3 3,231,892 - ygjM b3082 0.34 +1.0 3,232,179 + +0.9 3,232,179 + +0.1 3,232,181 + +0.6 3,232,186 + +0.6 3,232,189 - +0.2 3,232,233 + ygjN b3083 0.22 +0.1 3,232,267 + ygjN b3083 0.33 +0.6 3,232,272 - ygjN b3083 0.35 +0.6 3,232,302 + ygjN b3083 0.44 +0.0 3,232,394 + ygjN b3083 0.73 -1.2 3,232,416 + ygjN b3083 0.80 -0.2 3,232,465 - +0.6 3,232,465 - +0.2 3,232,465 - +0.7 3,232,471 + +1.0 3,232,603 + -0.6 3,232,603 + -0.2 3,232,704 - +0.8 3,232,704 - -0.0 3,232,783 + +0.8 3,232,783 + +0.4 3,232,815 + +0.3 3,232,830 - -0.7 3,232,862 + -1.2 3,232,862 + -0.6 3,232,924 - ygjO b3084 0.14 -0.3 3,232,967 - ygjO b3084 0.18 +0.1 3,232,979 - ygjO b3084 0.19 -0.5 3,232,979 - ygjO b3084 0.19 -0.1 3,232,999 - ygjO b3084 0.21 -0.3 3,232,999 - ygjO b3084 0.21 -4.9
Or see this region's nucleotide sequence