Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoF and nuoE overlap by 4 nucleotides nuoE and nuoC are separated by 2 nucleotides nuoC and nuoB are separated by 105 nucleotides nuoB and nuoA are separated by 15 nucleotides
b2284: nuoF - NADH:ubiquinone oxidoreductase, chain F (NCBI), at 2,398,240 to 2,399,577
nuoF
b2285: nuoE - NADH dehydrogenase subunit E (NCBI), at 2,399,574 to 2,400,074
nuoE
b2286: nuoC - NADH:ubiquinone oxidoreductase, chain C,D (RefSeq), at 2,400,077 to 2,401,867
nuoC
b2287: nuoB - NADH dehydrogenase subunit B (NCBI), at 2,401,973 to 2,402,635
nuoB
b2288: nuoA - NADH dehydrogenase I chain A (VIMSS), at 2,402,651 to 2,403,094
nuoA
Position (kb)
2400
2401
2402 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2399.113 kb on - strand, within nuoF at 2399.283 kb on - strand, within nuoF at 2399.293 kb on - strand, within nuoF at 2399.314 kb on - strand, within nuoF at 2399.405 kb on - strand, within nuoF at 2399.405 kb on - strand, within nuoF at 2399.406 kb on - strand, within nuoF at 2399.461 kb on + strand at 2399.461 kb on + strand at 2399.508 kb on - strand at 2399.626 kb on + strand at 2399.626 kb on + strand, within nuoE at 2399.725 kb on + strand, within nuoE at 2399.863 kb on - strand, within nuoE at 2399.948 kb on - strand, within nuoE at 2399.972 kb on + strand, within nuoE at 2400.031 kb on - strand at 2400.113 kb on + strand at 2400.113 kb on + strand at 2400.221 kb on - strand at 2400.221 kb on - strand at 2400.254 kb on + strand at 2400.254 kb on - strand at 2400.327 kb on - strand, within nuoC at 2400.349 kb on - strand, within nuoC at 2400.357 kb on - strand, within nuoC at 2400.431 kb on + strand, within nuoC at 2400.565 kb on - strand, within nuoC at 2400.683 kb on - strand, within nuoC at 2400.683 kb on - strand, within nuoC at 2401.060 kb on + strand, within nuoC at 2401.073 kb on - strand, within nuoC at 2401.073 kb on - strand, within nuoC at 2401.078 kb on - strand, within nuoC at 2401.197 kb on + strand, within nuoC at 2401.420 kb on - strand, within nuoC at 2401.492 kb on + strand, within nuoC at 2401.492 kb on + strand, within nuoC at 2401.504 kb on - strand, within nuoC at 2401.625 kb on + strand, within nuoC at 2401.665 kb on + strand, within nuoC at 2401.730 kb on - strand at 2401.730 kb on - strand at 2401.732 kb on - strand at 2401.732 kb on - strand at 2401.762 kb on - strand at 2401.767 kb on - strand at 2402.000 kb on + strand at 2402.085 kb on - strand, within nuoB at 2402.169 kb on + strand, within nuoB at 2402.169 kb on + strand, within nuoB at 2402.179 kb on - strand, within nuoB at 2402.212 kb on - strand, within nuoB at 2402.284 kb on + strand, within nuoB at 2402.378 kb on - strand, within nuoB at 2402.386 kb on - strand, within nuoB at 2402.386 kb on - strand, within nuoB at 2402.434 kb on + strand, within nuoB at 2402.434 kb on + strand, within nuoB at 2402.583 kb on + strand at 2402.621 kb on - strand at 2402.624 kb on + strand at 2402.662 kb on + strand at 2402.662 kb on + strand at 2402.665 kb on + strand at 2402.665 kb on + strand at 2402.694 kb on - strand at 2402.723 kb on + strand, within nuoA at 2402.723 kb on + strand, within nuoA at 2402.745 kb on + strand at 2402.805 kb on - strand, within nuoA at 2402.805 kb on - strand, within nuoA at 2402.819 kb on + strand, within nuoA
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 2,399,113 - nuoF b2284 0.65 -0.8 2,399,283 - nuoF b2284 0.78 -2.3 2,399,293 - nuoF b2284 0.79 -1.8 2,399,314 - nuoF b2284 0.80 -2.1 2,399,405 - nuoF b2284 0.87 -1.4 2,399,405 - nuoF b2284 0.87 -2.3 2,399,406 - nuoF b2284 0.87 -0.3 2,399,461 + -0.5 2,399,461 + -0.1 2,399,508 - +0.3 2,399,626 + +0.5 2,399,626 + nuoE b2285 0.10 +1.6 2,399,725 + nuoE b2285 0.30 -0.9 2,399,863 - nuoE b2285 0.58 +0.9 2,399,948 - nuoE b2285 0.75 -1.7 2,399,972 + nuoE b2285 0.79 +0.4 2,400,031 - -1.5 2,400,113 + -2.1 2,400,113 + -1.9 2,400,221 - -1.8 2,400,221 - -2.4 2,400,254 + -2.7 2,400,254 - +0.3 2,400,327 - nuoC b2286 0.14 -2.4 2,400,349 - nuoC b2286 0.15 -3.5 2,400,357 - nuoC b2286 0.16 -0.3 2,400,431 + nuoC b2286 0.20 -0.2 2,400,565 - nuoC b2286 0.27 -1.7 2,400,683 - nuoC b2286 0.34 -0.5 2,400,683 - nuoC b2286 0.34 -1.1 2,401,060 + nuoC b2286 0.55 +0.6 2,401,073 - nuoC b2286 0.56 +0.1 2,401,073 - nuoC b2286 0.56 -1.6 2,401,078 - nuoC b2286 0.56 -0.9 2,401,197 + nuoC b2286 0.63 -0.8 2,401,420 - nuoC b2286 0.75 -2.2 2,401,492 + nuoC b2286 0.79 +0.3 2,401,492 + nuoC b2286 0.79 -0.2 2,401,504 - nuoC b2286 0.80 -0.1 2,401,625 + nuoC b2286 0.86 -2.5 2,401,665 + nuoC b2286 0.89 -1.4 2,401,730 - -0.4 2,401,730 - -1.2 2,401,732 - +0.1 2,401,732 - -1.3 2,401,762 - -2.1 2,401,767 - -3.3 2,402,000 + -0.7 2,402,085 - nuoB b2287 0.17 +0.9 2,402,169 + nuoB b2287 0.30 -0.9 2,402,169 + nuoB b2287 0.30 -0.1 2,402,179 - nuoB b2287 0.31 -0.4 2,402,212 - nuoB b2287 0.36 -0.9 2,402,284 + nuoB b2287 0.47 +1.2 2,402,378 - nuoB b2287 0.61 +0.2 2,402,386 - nuoB b2287 0.62 -0.1 2,402,386 - nuoB b2287 0.62 -1.1 2,402,434 + nuoB b2287 0.70 -2.6 2,402,434 + nuoB b2287 0.70 -0.8 2,402,583 + -0.4 2,402,621 - +1.2 2,402,624 + -1.4 2,402,662 + +0.1 2,402,662 + -0.4 2,402,665 + -0.1 2,402,665 + -1.5 2,402,694 - -0.3 2,402,723 + nuoA b2288 0.16 +0.5 2,402,723 + nuoA b2288 0.16 +0.9 2,402,745 + +0.9 2,402,805 - nuoA b2288 0.35 +2.5 2,402,805 - nuoA b2288 0.35 -1.1 2,402,819 + nuoA b2288 0.38 -1.5
Or see this region's nucleotide sequence