Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoL and nuoK overlap by 4 nucleotides nuoK and nuoJ overlap by 4 nucleotides nuoJ and nuoI are separated by 11 nucleotides nuoI and nuoH are separated by 14 nucleotides
b2278: nuoL - NADH dehydrogenase subunit L (NCBI), at 2,391,227 to 2,393,068
nuoL
b2279: nuoK - NADH dehydrogenase subunit K (NCBI), at 2,393,065 to 2,393,367
nuoK
b2280: nuoJ - NADH dehydrogenase subunit J (NCBI), at 2,393,364 to 2,393,918
nuoJ
b2281: nuoI - NADH dehydrogenase subunit I (NCBI), at 2,393,930 to 2,394,472
nuoI
b2282: nuoH - NADH dehydrogenase subunit H (NCBI), at 2,394,487 to 2,395,464
nuoH
Position (kb)
2393
2394 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2392.403 kb on - strand, within nuoL at 2392.403 kb on - strand, within nuoL at 2392.419 kb on - strand, within nuoL at 2392.424 kb on - strand, within nuoL at 2392.488 kb on - strand, within nuoL at 2392.498 kb on - strand, within nuoL at 2392.537 kb on - strand, within nuoL at 2392.555 kb on - strand, within nuoL at 2392.578 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.620 kb on + strand, within nuoL at 2392.627 kb on + strand, within nuoL at 2392.694 kb on - strand, within nuoL at 2392.723 kb on + strand, within nuoL at 2392.723 kb on + strand, within nuoL at 2392.728 kb on + strand, within nuoL at 2392.728 kb on + strand, within nuoL at 2392.771 kb on + strand, within nuoL at 2392.776 kb on + strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.874 kb on - strand, within nuoL at 2392.895 kb on - strand at 2393.077 kb on + strand at 2393.077 kb on + strand at 2393.106 kb on + strand, within nuoK at 2393.152 kb on - strand, within nuoK at 2393.213 kb on - strand, within nuoK at 2393.323 kb on + strand, within nuoK at 2393.346 kb on + strand at 2393.410 kb on + strand at 2393.412 kb on + strand at 2393.412 kb on + strand at 2393.419 kb on + strand at 2393.456 kb on - strand, within nuoJ at 2393.463 kb on - strand, within nuoJ at 2393.530 kb on - strand, within nuoJ at 2393.530 kb on - strand, within nuoJ at 2393.536 kb on + strand, within nuoJ at 2393.559 kb on + strand, within nuoJ at 2393.581 kb on - strand, within nuoJ at 2393.586 kb on - strand, within nuoJ at 2393.606 kb on + strand, within nuoJ at 2393.611 kb on - strand, within nuoJ at 2393.617 kb on + strand, within nuoJ at 2393.637 kb on + strand, within nuoJ at 2393.638 kb on + strand, within nuoJ at 2393.638 kb on + strand, within nuoJ at 2393.645 kb on + strand, within nuoJ at 2393.724 kb on + strand, within nuoJ at 2393.731 kb on - strand, within nuoJ at 2393.839 kb on - strand, within nuoJ at 2393.840 kb on + strand, within nuoJ at 2393.876 kb on + strand at 2393.898 kb on - strand at 2393.915 kb on + strand at 2393.915 kb on + strand at 2393.967 kb on - strand at 2393.967 kb on - strand at 2394.043 kb on + strand, within nuoI at 2394.124 kb on - strand, within nuoI at 2394.163 kb on + strand, within nuoI at 2394.320 kb on + strand, within nuoI at 2394.328 kb on - strand, within nuoI at 2394.345 kb on - strand, within nuoI at 2394.347 kb on - strand, within nuoI at 2394.411 kb on - strand, within nuoI at 2394.482 kb on - strand at 2394.495 kb on - strand at 2394.495 kb on - strand at 2394.603 kb on + strand, within nuoH at 2394.603 kb on + strand, within nuoH at 2394.624 kb on + strand, within nuoH at 2394.634 kb on - strand, within nuoH at 2394.712 kb on - strand at 2394.804 kb on + strand, within nuoH at 2394.815 kb on + strand, within nuoH at 2394.834 kb on + strand, within nuoH
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 2,392,403 - nuoL b2278 0.64 -1.1 2,392,403 - nuoL b2278 0.64 +1.9 2,392,419 - nuoL b2278 0.65 -1.6 2,392,424 - nuoL b2278 0.65 -2.8 2,392,488 - nuoL b2278 0.68 -1.1 2,392,498 - nuoL b2278 0.69 -0.4 2,392,537 - nuoL b2278 0.71 -2.1 2,392,555 - nuoL b2278 0.72 -1.2 2,392,578 + nuoL b2278 0.73 -2.3 2,392,593 + nuoL b2278 0.74 -0.4 2,392,593 + nuoL b2278 0.74 -2.1 2,392,593 + nuoL b2278 0.74 -1.9 2,392,601 - nuoL b2278 0.75 +0.2 2,392,601 - nuoL b2278 0.75 +0.9 2,392,601 - nuoL b2278 0.75 +0.3 2,392,620 + nuoL b2278 0.76 -0.5 2,392,627 + nuoL b2278 0.76 -2.5 2,392,694 - nuoL b2278 0.80 -0.8 2,392,723 + nuoL b2278 0.81 -0.3 2,392,723 + nuoL b2278 0.81 -1.9 2,392,728 + nuoL b2278 0.81 -3.1 2,392,728 + nuoL b2278 0.81 -1.8 2,392,771 + nuoL b2278 0.84 -0.3 2,392,776 + nuoL b2278 0.84 -0.5 2,392,831 - nuoL b2278 0.87 -1.3 2,392,831 - nuoL b2278 0.87 +0.2 2,392,831 - nuoL b2278 0.87 -1.3 2,392,831 - nuoL b2278 0.87 -0.8 2,392,874 - nuoL b2278 0.89 -2.6 2,392,895 - -0.5 2,393,077 + -1.1 2,393,077 + -1.5 2,393,106 + nuoK b2279 0.14 -1.1 2,393,152 - nuoK b2279 0.29 +0.5 2,393,213 - nuoK b2279 0.49 +0.0 2,393,323 + nuoK b2279 0.85 -3.7 2,393,346 + -3.7 2,393,410 + -0.7 2,393,412 + -1.2 2,393,412 + -2.5 2,393,419 + -1.4 2,393,456 - nuoJ b2280 0.17 -0.9 2,393,463 - nuoJ b2280 0.18 -0.6 2,393,530 - nuoJ b2280 0.30 -3.2 2,393,530 - nuoJ b2280 0.30 -4.0 2,393,536 + nuoJ b2280 0.31 -1.7 2,393,559 + nuoJ b2280 0.35 -0.9 2,393,581 - nuoJ b2280 0.39 -2.3 2,393,586 - nuoJ b2280 0.40 -2.5 2,393,606 + nuoJ b2280 0.44 -1.8 2,393,611 - nuoJ b2280 0.45 -2.3 2,393,617 + nuoJ b2280 0.46 -0.6 2,393,637 + nuoJ b2280 0.49 -3.0 2,393,638 + nuoJ b2280 0.49 +0.9 2,393,638 + nuoJ b2280 0.49 -2.3 2,393,645 + nuoJ b2280 0.51 -2.2 2,393,724 + nuoJ b2280 0.65 -1.6 2,393,731 - nuoJ b2280 0.66 -0.8 2,393,839 - nuoJ b2280 0.86 -1.5 2,393,840 + nuoJ b2280 0.86 -0.7 2,393,876 + -1.4 2,393,898 - -1.9 2,393,915 + -2.0 2,393,915 + -1.1 2,393,967 - -1.3 2,393,967 - -1.0 2,394,043 + nuoI b2281 0.21 -0.1 2,394,124 - nuoI b2281 0.36 -0.1 2,394,163 + nuoI b2281 0.43 -0.8 2,394,320 + nuoI b2281 0.72 -1.2 2,394,328 - nuoI b2281 0.73 +0.2 2,394,345 - nuoI b2281 0.76 -1.7 2,394,347 - nuoI b2281 0.77 -0.9 2,394,411 - nuoI b2281 0.89 -1.2 2,394,482 - -0.3 2,394,495 - -0.1 2,394,495 - -0.1 2,394,603 + nuoH b2282 0.12 +0.9 2,394,603 + nuoH b2282 0.12 -1.8 2,394,624 + nuoH b2282 0.14 -1.7 2,394,634 - nuoH b2282 0.15 -0.7 2,394,712 - +1.9 2,394,804 + nuoH b2282 0.32 +0.1 2,394,815 + nuoH b2282 0.34 -2.7 2,394,834 + nuoH b2282 0.35 -1.9
Or see this region's nucleotide sequence