Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2250 and yfaO are separated by 278 nucleotides yfaO and ais are separated by 38 nucleotides ais and arnB are separated by 307 nucleotides
b2250: b2250 - orf, hypothetical protein (VIMSS), at 2,361,755 to 2,362,297
b2250
b2251: yfaO - predicted NUDIX hydrolase (NCBI), at 2,362,576 to 2,363,001
yfaO
b2252: ais - hypothetical protein (NCBI), at 2,363,040 to 2,363,642
ais
b2253: arnB - uridine 5'-(beta-1-threo-pentapyranosyl-4-ulose diphosphate) aminotransferase, PLP-dependent (RefSeq), at 2,363,950 to 2,365,089
arnB
Position (kb)
2363
2364 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2362.040 kb on + strand, within b2250 at 2362.209 kb on + strand, within b2250 at 2362.214 kb on + strand, within b2250 at 2362.221 kb on + strand, within b2250 at 2362.239 kb on - strand, within b2250 at 2362.244 kb on - strand at 2362.244 kb on - strand at 2362.246 kb on + strand at 2362.307 kb on + strand at 2362.307 kb on + strand at 2362.316 kb on - strand at 2362.345 kb on + strand at 2362.345 kb on + strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.357 kb on + strand at 2362.361 kb on + strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.365 kb on - strand at 2362.366 kb on + strand at 2362.366 kb on + strand at 2362.399 kb on + strand at 2362.475 kb on + strand at 2362.486 kb on - strand at 2362.488 kb on - strand at 2362.488 kb on - strand at 2362.528 kb on + strand at 2362.531 kb on - strand at 2362.533 kb on + strand at 2362.534 kb on + strand at 2362.534 kb on + strand at 2362.541 kb on - strand at 2362.586 kb on + strand at 2362.590 kb on - strand at 2362.590 kb on - strand at 2362.591 kb on + strand at 2362.599 kb on - strand at 2362.599 kb on - strand at 2362.600 kb on - strand at 2362.600 kb on - strand at 2362.601 kb on - strand at 2362.601 kb on - strand at 2362.634 kb on + strand, within yfaO at 2362.688 kb on - strand, within yfaO at 2362.691 kb on + strand, within yfaO at 2362.691 kb on + strand, within yfaO at 2362.691 kb on + strand, within yfaO at 2362.691 kb on + strand, within yfaO at 2362.691 kb on + strand, within yfaO at 2362.699 kb on - strand, within yfaO at 2362.757 kb on + strand, within yfaO at 2362.772 kb on + strand, within yfaO at 2362.772 kb on + strand, within yfaO at 2362.815 kb on + strand, within yfaO at 2362.815 kb on + strand, within yfaO at 2362.822 kb on - strand, within yfaO at 2362.822 kb on - strand, within yfaO at 2362.919 kb on - strand, within yfaO at 2362.931 kb on - strand, within yfaO at 2362.994 kb on + strand at 2363.002 kb on + strand at 2363.002 kb on + strand at 2363.003 kb on + strand at 2363.008 kb on + strand at 2363.008 kb on + strand at 2363.082 kb on + strand at 2363.082 kb on + strand at 2363.084 kb on + strand at 2363.088 kb on + strand at 2363.093 kb on + strand at 2363.093 kb on + strand at 2363.126 kb on - strand, within ais at 2363.142 kb on - strand, within ais at 2363.153 kb on + strand, within ais at 2363.244 kb on + strand, within ais at 2363.244 kb on + strand, within ais at 2363.275 kb on + strand, within ais at 2363.275 kb on + strand, within ais at 2363.299 kb on + strand, within ais at 2363.320 kb on + strand, within ais at 2363.328 kb on - strand, within ais at 2363.373 kb on - strand, within ais at 2363.375 kb on + strand, within ais at 2363.375 kb on + strand, within ais at 2363.375 kb on + strand, within ais at 2363.383 kb on - strand, within ais at 2363.385 kb on + strand, within ais at 2363.387 kb on + strand, within ais at 2363.387 kb on + strand, within ais at 2363.387 kb on + strand, within ais at 2363.387 kb on + strand, within ais at 2363.393 kb on - strand, within ais at 2363.395 kb on - strand, within ais at 2363.404 kb on - strand, within ais at 2363.418 kb on + strand, within ais at 2363.418 kb on + strand, within ais at 2363.426 kb on + strand, within ais at 2363.427 kb on + strand, within ais at 2363.430 kb on - strand, within ais at 2363.434 kb on - strand, within ais at 2363.445 kb on - strand, within ais at 2363.451 kb on + strand, within ais at 2363.468 kb on + strand, within ais at 2363.468 kb on + strand, within ais at 2363.492 kb on + strand, within ais at 2363.500 kb on - strand, within ais at 2363.505 kb on - strand, within ais at 2363.505 kb on - strand, within ais at 2363.507 kb on + strand, within ais at 2363.507 kb on + strand, within ais at 2363.510 kb on - strand, within ais at 2363.525 kb on + strand, within ais at 2363.533 kb on + strand, within ais at 2363.533 kb on + strand, within ais at 2363.536 kb on + strand, within ais at 2363.536 kb on + strand, within ais at 2363.536 kb on + strand, within ais at 2363.536 kb on + strand, within ais at 2363.536 kb on + strand, within ais at 2363.536 kb on + strand, within ais at 2363.543 kb on - strand, within ais at 2363.544 kb on - strand, within ais at 2363.544 kb on - strand, within ais at 2363.544 kb on - strand, within ais at 2363.549 kb on - strand, within ais at 2363.554 kb on + strand, within ais at 2363.562 kb on - strand, within ais at 2363.645 kb on + strand at 2363.669 kb on + strand at 2363.699 kb on - strand at 2363.855 kb on - strand at 2364.000 kb on + strand at 2364.013 kb on - strand at 2364.016 kb on + strand at 2364.026 kb on - strand at 2364.036 kb on - strand at 2364.041 kb on - strand at 2364.041 kb on - strand at 2364.168 kb on - strand, within arnB at 2364.168 kb on - strand, within arnB at 2364.185 kb on - strand, within arnB at 2364.192 kb on + strand, within arnB at 2364.195 kb on - strand, within arnB at 2364.244 kb on - strand, within arnB at 2364.301 kb on + strand, within arnB at 2364.314 kb on + strand, within arnB at 2364.333 kb on + strand, within arnB at 2364.514 kb on - strand, within arnB at 2364.522 kb on + strand, within arnB at 2364.590 kb on - strand, within arnB at 2364.590 kb on - strand, within arnB
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 2,362,040 + b2250 b2250 0.52 -2.5 2,362,209 + b2250 b2250 0.84 -0.4 2,362,214 + b2250 b2250 0.85 -0.2 2,362,221 + b2250 b2250 0.86 +0.1 2,362,239 - b2250 b2250 0.89 +0.7 2,362,244 - +0.2 2,362,244 - +0.0 2,362,246 + -0.4 2,362,307 + -1.8 2,362,307 + -0.4 2,362,316 - +1.0 2,362,345 + +0.6 2,362,345 + +0.9 2,362,351 - +0.6 2,362,351 - -0.1 2,362,351 - -0.6 2,362,351 - -0.1 2,362,351 - +0.7 2,362,351 - +0.2 2,362,351 - -0.6 2,362,351 - -0.1 2,362,351 - -0.7 2,362,351 - +0.8 2,362,353 + +0.2 2,362,353 + -0.1 2,362,353 + -0.3 2,362,353 + +0.3 2,362,353 + +0.5 2,362,353 + +0.4 2,362,357 + +0.3 2,362,361 + -0.8 2,362,361 - -0.1 2,362,361 - -0.5 2,362,361 - +2.2 2,362,361 - -0.5 2,362,361 - -0.6 2,362,361 - -0.9 2,362,361 - +0.5 2,362,365 - +0.4 2,362,366 + -0.3 2,362,366 + -1.7 2,362,399 + +0.4 2,362,475 + +0.4 2,362,486 - -0.5 2,362,488 - +0.9 2,362,488 - -0.1 2,362,528 + -0.6 2,362,531 - -1.5 2,362,533 + +0.1 2,362,534 + +1.2 2,362,534 + -0.2 2,362,541 - -2.3 2,362,586 + -1.0 2,362,590 - -1.7 2,362,590 - -0.5 2,362,591 + -0.7 2,362,599 - +0.6 2,362,599 - -1.8 2,362,600 - +2.2 2,362,600 - +0.9 2,362,601 - -0.3 2,362,601 - +0.4 2,362,634 + yfaO b2251 0.14 -0.6 2,362,688 - yfaO b2251 0.26 +0.9 2,362,691 + yfaO b2251 0.27 -0.5 2,362,691 + yfaO b2251 0.27 +0.2 2,362,691 + yfaO b2251 0.27 -0.0 2,362,691 + yfaO b2251 0.27 +0.7 2,362,691 + yfaO b2251 0.27 -0.5 2,362,699 - yfaO b2251 0.29 -0.9 2,362,757 + yfaO b2251 0.42 +1.0 2,362,772 + yfaO b2251 0.46 +0.3 2,362,772 + yfaO b2251 0.46 -0.4 2,362,815 + yfaO b2251 0.56 +0.5 2,362,815 + yfaO b2251 0.56 -0.2 2,362,822 - yfaO b2251 0.58 +0.9 2,362,822 - yfaO b2251 0.58 +1.5 2,362,919 - yfaO b2251 0.81 -2.3 2,362,931 - yfaO b2251 0.83 +0.9 2,362,994 + -1.1 2,363,002 + +0.2 2,363,002 + -2.3 2,363,003 + -0.8 2,363,008 + -1.4 2,363,008 + -1.1 2,363,082 + +0.4 2,363,082 + +0.9 2,363,084 + +0.9 2,363,088 + -1.4 2,363,093 + -0.1 2,363,093 + -3.0 2,363,126 - ais b2252 0.14 -2.7 2,363,142 - ais b2252 0.17 -1.7 2,363,153 + ais b2252 0.19 +0.9 2,363,244 + ais b2252 0.34 -1.4 2,363,244 + ais b2252 0.34 +0.2 2,363,275 + ais b2252 0.39 -2.3 2,363,275 + ais b2252 0.39 -2.5 2,363,299 + ais b2252 0.43 -0.4 2,363,320 + ais b2252 0.46 +0.1 2,363,328 - ais b2252 0.48 +1.9 2,363,373 - ais b2252 0.55 +0.1 2,363,375 + ais b2252 0.56 -1.1 2,363,375 + ais b2252 0.56 -0.4 2,363,375 + ais b2252 0.56 +0.1 2,363,383 - ais b2252 0.57 +1.9 2,363,385 + ais b2252 0.57 +0.9 2,363,387 + ais b2252 0.58 -2.3 2,363,387 + ais b2252 0.58 -0.7 2,363,387 + ais b2252 0.58 -0.1 2,363,387 + ais b2252 0.58 -0.7 2,363,393 - ais b2252 0.59 -1.7 2,363,395 - ais b2252 0.59 +0.9 2,363,404 - ais b2252 0.60 -1.4 2,363,418 + ais b2252 0.63 +0.3 2,363,418 + ais b2252 0.63 -1.0 2,363,426 + ais b2252 0.64 -2.1 2,363,427 + ais b2252 0.64 -2.5 2,363,430 - ais b2252 0.65 -0.4 2,363,434 - ais b2252 0.65 +0.9 2,363,445 - ais b2252 0.67 -0.3 2,363,451 + ais b2252 0.68 +0.9 2,363,468 + ais b2252 0.71 -2.1 2,363,468 + ais b2252 0.71 +0.5 2,363,492 + ais b2252 0.75 +0.9 2,363,500 - ais b2252 0.76 +0.9 2,363,505 - ais b2252 0.77 -1.4 2,363,505 - ais b2252 0.77 -1.4 2,363,507 + ais b2252 0.77 -0.7 2,363,507 + ais b2252 0.77 +0.9 2,363,510 - ais b2252 0.78 +0.9 2,363,525 + ais b2252 0.80 -0.1 2,363,533 + ais b2252 0.82 -1.8 2,363,533 + ais b2252 0.82 -0.9 2,363,536 + ais b2252 0.82 -1.9 2,363,536 + ais b2252 0.82 -0.7 2,363,536 + ais b2252 0.82 -0.1 2,363,536 + ais b2252 0.82 +0.9 2,363,536 + ais b2252 0.82 -0.4 2,363,536 + ais b2252 0.82 +0.9 2,363,543 - ais b2252 0.83 -0.8 2,363,544 - ais b2252 0.84 +0.3 2,363,544 - ais b2252 0.84 +0.9 2,363,544 - ais b2252 0.84 +0.9 2,363,549 - ais b2252 0.84 -1.1 2,363,554 + ais b2252 0.85 -1.1 2,363,562 - ais b2252 0.87 +0.9 2,363,645 + -0.1 2,363,669 + +0.5 2,363,699 - +0.9 2,363,855 - +0.9 2,364,000 + +0.9 2,364,013 - +0.6 2,364,016 + +0.9 2,364,026 - +1.0 2,364,036 - +0.5 2,364,041 - -0.0 2,364,041 - +0.1 2,364,168 - arnB b2253 0.19 +0.1 2,364,168 - arnB b2253 0.19 +0.1 2,364,185 - arnB b2253 0.21 +0.1 2,364,192 + arnB b2253 0.21 +0.4 2,364,195 - arnB b2253 0.21 -1.1 2,364,244 - arnB b2253 0.26 +0.3 2,364,301 + arnB b2253 0.31 +0.1 2,364,314 + arnB b2253 0.32 +0.1 2,364,333 + arnB b2253 0.34 +0.1 2,364,514 - arnB b2253 0.49 -0.2 2,364,522 + arnB b2253 0.50 -0.5 2,364,590 - arnB b2253 0.56 -1.7 2,364,590 - arnB b2253 0.56 -0.1
Or see this region's nucleotide sequence