Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2174 and spr are separated by 411 nucleotides spr and rtn are separated by 180 nucleotides
b2174: b2174 - orf, hypothetical protein (VIMSS), at 2,266,876 to 2,267,589
b2174
b2175: spr - predicted peptidase, outer membrane lipoprotein (NCBI), at 2,268,001 to 2,268,567
spr
b2176: rtn - hypothetical protein (NCBI), at 2,268,748 to 2,270,304
rtn
Position (kb)
2268
2269 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2267.048 kb on - strand, within b2174 at 2267.073 kb on - strand, within b2174 at 2267.090 kb on - strand, within b2174 at 2267.090 kb on - strand, within b2174 at 2267.171 kb on - strand, within b2174 at 2267.174 kb on + strand, within b2174 at 2267.185 kb on + strand, within b2174 at 2267.185 kb on + strand, within b2174 at 2267.185 kb on + strand, within b2174 at 2267.185 kb on + strand, within b2174 at 2267.231 kb on + strand, within b2174 at 2267.231 kb on + strand, within b2174 at 2267.323 kb on - strand, within b2174 at 2267.428 kb on + strand, within b2174 at 2267.457 kb on - strand, within b2174 at 2267.490 kb on - strand, within b2174 at 2267.496 kb on + strand, within b2174 at 2267.496 kb on + strand, within b2174 at 2267.496 kb on + strand, within b2174 at 2267.497 kb on + strand, within b2174 at 2267.515 kb on + strand, within b2174 at 2267.515 kb on + strand, within b2174 at 2267.522 kb on - strand at 2267.523 kb on - strand at 2267.535 kb on - strand at 2267.618 kb on + strand at 2267.628 kb on - strand at 2267.628 kb on - strand at 2267.638 kb on - strand at 2267.638 kb on - strand at 2267.644 kb on - strand at 2267.644 kb on - strand at 2267.645 kb on + strand at 2267.659 kb on - strand at 2267.659 kb on - strand at 2267.661 kb on - strand at 2267.793 kb on - strand at 2267.806 kb on - strand at 2267.817 kb on + strand at 2267.819 kb on - strand at 2267.853 kb on + strand at 2268.026 kb on + strand at 2268.026 kb on + strand at 2268.056 kb on + strand at 2268.058 kb on - strand, within spr at 2268.072 kb on - strand, within spr at 2268.142 kb on - strand, within spr at 2268.216 kb on + strand, within spr at 2268.216 kb on + strand, within spr at 2268.216 kb on + strand, within spr at 2268.306 kb on - strand, within spr at 2268.416 kb on - strand, within spr at 2268.468 kb on - strand, within spr at 2268.516 kb on + strand at 2268.516 kb on + strand at 2268.536 kb on - strand at 2268.574 kb on - strand at 2268.574 kb on - strand at 2268.579 kb on + strand at 2268.594 kb on - strand at 2268.660 kb on + strand at 2268.660 kb on + strand at 2268.684 kb on + strand at 2268.694 kb on - strand at 2268.694 kb on - strand at 2268.813 kb on - strand at 2268.813 kb on - strand at 2268.895 kb on + strand at 2268.895 kb on + strand at 2268.933 kb on + strand, within rtn at 2268.933 kb on + strand, within rtn at 2268.971 kb on - strand, within rtn at 2269.018 kb on - strand, within rtn at 2269.083 kb on - strand, within rtn at 2269.083 kb on - strand, within rtn at 2269.086 kb on + strand, within rtn at 2269.147 kb on + strand, within rtn at 2269.147 kb on + strand, within rtn at 2269.147 kb on + strand, within rtn at 2269.155 kb on - strand, within rtn at 2269.155 kb on - strand, within rtn at 2269.338 kb on + strand, within rtn at 2269.358 kb on + strand, within rtn at 2269.358 kb on + strand, within rtn at 2269.415 kb on - strand, within rtn at 2269.425 kb on - strand, within rtn at 2269.447 kb on - strand, within rtn at 2269.447 kb on - strand, within rtn at 2269.511 kb on - strand, within rtn at 2269.511 kb on - strand, within rtn at 2269.516 kb on - strand, within rtn
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 2,267,048 - b2174 b2174 0.24 -0.7 2,267,073 - b2174 b2174 0.28 -0.2 2,267,090 - b2174 b2174 0.30 -0.5 2,267,090 - b2174 b2174 0.30 -2.9 2,267,171 - b2174 b2174 0.41 +0.4 2,267,174 + b2174 b2174 0.42 -0.3 2,267,185 + b2174 b2174 0.43 +0.4 2,267,185 + b2174 b2174 0.43 +0.2 2,267,185 + b2174 b2174 0.43 -0.7 2,267,185 + b2174 b2174 0.43 +0.5 2,267,231 + b2174 b2174 0.50 +0.1 2,267,231 + b2174 b2174 0.50 -0.3 2,267,323 - b2174 b2174 0.63 -0.7 2,267,428 + b2174 b2174 0.77 +1.1 2,267,457 - b2174 b2174 0.81 +0.3 2,267,490 - b2174 b2174 0.86 +0.7 2,267,496 + b2174 b2174 0.87 +0.6 2,267,496 + b2174 b2174 0.87 -0.3 2,267,496 + b2174 b2174 0.87 -1.4 2,267,497 + b2174 b2174 0.87 -1.6 2,267,515 + b2174 b2174 0.89 -0.4 2,267,515 + b2174 b2174 0.89 -0.0 2,267,522 - -1.4 2,267,523 - -0.3 2,267,535 - +0.3 2,267,618 + -1.4 2,267,628 - -1.1 2,267,628 - +3.2 2,267,638 - -0.3 2,267,638 - -1.2 2,267,644 - -1.7 2,267,644 - +0.3 2,267,645 + +1.9 2,267,659 - -0.6 2,267,659 - +0.4 2,267,661 - -0.7 2,267,793 - +0.2 2,267,806 - -0.5 2,267,817 + +0.6 2,267,819 - -0.2 2,267,853 + +1.2 2,268,026 + -2.7 2,268,026 + -1.9 2,268,056 + -1.3 2,268,058 - spr b2175 0.10 -2.7 2,268,072 - spr b2175 0.13 -1.7 2,268,142 - spr b2175 0.25 -1.5 2,268,216 + spr b2175 0.38 -1.8 2,268,216 + spr b2175 0.38 -2.9 2,268,216 + spr b2175 0.38 -1.5 2,268,306 - spr b2175 0.54 -1.1 2,268,416 - spr b2175 0.73 +0.9 2,268,468 - spr b2175 0.82 -1.1 2,268,516 + -2.4 2,268,516 + -1.1 2,268,536 - -0.1 2,268,574 - -0.7 2,268,574 - +2.7 2,268,579 + -0.1 2,268,594 - -0.7 2,268,660 + -1.7 2,268,660 + -1.9 2,268,684 + -0.9 2,268,694 - -0.4 2,268,694 - +0.2 2,268,813 - +0.0 2,268,813 - -0.6 2,268,895 + +0.9 2,268,895 + +0.6 2,268,933 + rtn b2176 0.12 +0.6 2,268,933 + rtn b2176 0.12 -0.8 2,268,971 - rtn b2176 0.14 +0.4 2,269,018 - rtn b2176 0.17 +1.2 2,269,083 - rtn b2176 0.22 -2.4 2,269,083 - rtn b2176 0.22 -0.5 2,269,086 + rtn b2176 0.22 +0.6 2,269,147 + rtn b2176 0.26 +0.1 2,269,147 + rtn b2176 0.26 -0.8 2,269,147 + rtn b2176 0.26 -0.0 2,269,155 - rtn b2176 0.26 -0.7 2,269,155 - rtn b2176 0.26 +0.2 2,269,338 + rtn b2176 0.38 +0.4 2,269,358 + rtn b2176 0.39 -0.9 2,269,358 + rtn b2176 0.39 -0.1 2,269,415 - rtn b2176 0.43 -0.8 2,269,425 - rtn b2176 0.43 +0.2 2,269,447 - rtn b2176 0.45 +0.1 2,269,447 - rtn b2176 0.45 -0.4 2,269,511 - rtn b2176 0.49 +0.0 2,269,511 - rtn b2176 0.49 +0.6 2,269,516 - rtn b2176 0.49 +0.5
Or see this region's nucleotide sequence