Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yedA and vsr overlap by 12 nucleotides vsr and dcm overlap by 20 nucleotides dcm and yedJ are separated by 66 nucleotides yedJ and b1963 are separated by 39 nucleotides
b1959: yedA - predicted inner membrane protein (NCBI), at 2,027,563 to 2,028,483
yedA
b1960: vsr - DNA mismatch endonuclease of very short patch repair (NCBI), at 2,028,472 to 2,028,942
vsr
b1961: dcm - DNA cytosine methylase (NCBI), at 2,028,923 to 2,030,341
dcm
b1962: yedJ - predicted phosphohydrolase (NCBI), at 2,030,408 to 2,031,103
yedJ
b1963: b1963 - orf, hypothetical protein (VIMSS), at 2,031,143 to 2,031,508
b1963
Position (kb)
2028
2029
2030
2031 Strain fitness (log2 ratio)
-1
0
1
2 at 2028.038 kb on + strand, within yedA at 2028.081 kb on - strand, within yedA at 2028.322 kb on + strand, within yedA at 2028.398 kb on + strand at 2028.717 kb on + strand, within vsr at 2028.727 kb on - strand, within vsr at 2028.727 kb on - strand, within vsr at 2028.753 kb on + strand, within vsr at 2028.753 kb on + strand, within vsr at 2028.819 kb on - strand, within vsr at 2028.819 kb on - strand, within vsr at 2028.943 kb on + strand at 2028.966 kb on + strand at 2028.966 kb on - strand at 2029.342 kb on + strand at 2029.361 kb on - strand, within dcm at 2030.164 kb on + strand, within dcm at 2030.226 kb on + strand at 2030.226 kb on + strand at 2030.231 kb on + strand at 2030.451 kb on + strand at 2030.564 kb on + strand, within yedJ at 2030.595 kb on + strand, within yedJ at 2030.690 kb on + strand, within yedJ at 2030.849 kb on + strand, within yedJ at 2031.000 kb on + strand, within yedJ at 2031.033 kb on - strand, within yedJ at 2031.117 kb on + strand at 2031.157 kb on + strand at 2031.157 kb on + strand at 2031.174 kb on + strand at 2031.179 kb on + strand at 2031.179 kb on + strand at 2031.181 kb on - strand, within b1963 at 2031.181 kb on - strand, within b1963 at 2031.226 kb on + strand, within b1963 at 2031.226 kb on + strand, within b1963 at 2031.244 kb on + strand, within b1963
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 2,028,038 + yedA b1959 0.52 -0.6 2,028,081 - yedA b1959 0.56 +2.5 2,028,322 + yedA b1959 0.82 -0.5 2,028,398 + -0.0 2,028,717 + vsr b1960 0.52 -1.7 2,028,727 - vsr b1960 0.54 -0.2 2,028,727 - vsr b1960 0.54 +0.1 2,028,753 + vsr b1960 0.60 -0.8 2,028,753 + vsr b1960 0.60 -0.0 2,028,819 - vsr b1960 0.74 +0.9 2,028,819 - vsr b1960 0.74 +0.4 2,028,943 + -0.0 2,028,966 + +0.4 2,028,966 - -0.8 2,029,342 + +0.6 2,029,361 - dcm b1961 0.31 -1.4 2,030,164 + dcm b1961 0.87 -1.5 2,030,226 + +1.3 2,030,226 + -1.3 2,030,231 + -0.1 2,030,451 + +0.6 2,030,564 + yedJ b1962 0.22 -0.1 2,030,595 + yedJ b1962 0.27 -0.5 2,030,690 + yedJ b1962 0.41 +0.4 2,030,849 + yedJ b1962 0.63 -0.7 2,031,000 + yedJ b1962 0.85 +0.2 2,031,033 - yedJ b1962 0.90 -0.8 2,031,117 + -0.1 2,031,157 + -0.6 2,031,157 + +0.7 2,031,174 + +0.3 2,031,179 + -0.5 2,031,179 + -1.6 2,031,181 - b1963 b1963 0.10 -0.3 2,031,181 - b1963 b1963 0.10 -1.6 2,031,226 + b1963 b1963 0.23 +0.4 2,031,226 + b1963 b1963 0.23 +0.6 2,031,244 + b1963 b1963 0.28 +1.1
Or see this region's nucleotide sequence