Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycbZ and ycbG are separated by 185 nucleotides ycbG and ompA are separated by 75 nucleotides ompA and sulA are separated by 356 nucleotides
b0955: ycbZ - predicted peptidase (NCBI), at 1,015,762 to 1,017,522
ycbZ
b0956: ycbG - hypothetical protein (NCBI), at 1,017,708 to 1,018,160
ycbG
b0957: ompA - outer membrane protein A (3a;II*;G;d) (NCBI), at 1,018,236 to 1,019,276
ompA
b0958: sulA - SOS cell division inhibitor (NCBI), at 1,019,633 to 1,020,142
sulA
Position (kb)
1018
1019
1020 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1017.303 kb on + strand, within ycbZ at 1017.321 kb on - strand, within ycbZ at 1017.335 kb on - strand, within ycbZ at 1017.425 kb on - strand at 1017.472 kb on + strand at 1017.607 kb on - strand at 1017.607 kb on - strand at 1018.221 kb on - strand at 1018.253 kb on - strand at 1018.253 kb on - strand at 1018.317 kb on - strand at 1018.327 kb on - strand at 1018.327 kb on - strand at 1018.327 kb on - strand at 1018.355 kb on - strand, within ompA at 1018.355 kb on - strand, within ompA at 1018.483 kb on - strand at 1018.549 kb on + strand, within ompA at 1018.649 kb on - strand, within ompA at 1018.740 kb on + strand, within ompA at 1018.759 kb on + strand, within ompA at 1018.987 kb on - strand, within ompA at 1019.129 kb on + strand, within ompA at 1019.195 kb on - strand at 1019.243 kb on + strand at 1019.247 kb on - strand at 1019.426 kb on - strand at 1019.426 kb on - strand at 1019.426 kb on - strand at 1019.430 kb on + strand at 1019.793 kb on - strand, within sulA at 1019.842 kb on + strand, within sulA at 1019.853 kb on + strand, within sulA at 1019.911 kb on - strand, within sulA at 1020.001 kb on - strand, within sulA at 1020.001 kb on - strand, within sulA at 1020.042 kb on + strand, within sulA at 1020.049 kb on + strand, within sulA at 1020.049 kb on + strand, within sulA at 1020.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 1,017,303 + ycbZ b0955 0.88 -0.3 1,017,321 - ycbZ b0955 0.89 +0.3 1,017,335 - ycbZ b0955 0.89 -0.3 1,017,425 - +0.2 1,017,472 + -1.2 1,017,607 - +0.2 1,017,607 - +0.8 1,018,221 - -1.0 1,018,253 - +0.1 1,018,253 - -0.7 1,018,317 - -4.0 1,018,327 - -1.4 1,018,327 - -4.3 1,018,327 - -2.7 1,018,355 - ompA b0957 0.11 -2.5 1,018,355 - ompA b0957 0.11 -1.5 1,018,483 - -2.8 1,018,549 + ompA b0957 0.30 +0.9 1,018,649 - ompA b0957 0.40 -0.5 1,018,740 + ompA b0957 0.48 -0.8 1,018,759 + ompA b0957 0.50 -1.7 1,018,987 - ompA b0957 0.72 -1.7 1,019,129 + ompA b0957 0.86 -2.6 1,019,195 - -2.5 1,019,243 + +1.9 1,019,247 - -2.2 1,019,426 - +1.0 1,019,426 - -1.3 1,019,426 - +0.3 1,019,430 + +0.1 1,019,793 - sulA b0958 0.31 +0.9 1,019,842 + sulA b0958 0.41 -1.2 1,019,853 + sulA b0958 0.43 +0.8 1,019,911 - sulA b0958 0.55 +0.6 1,020,001 - sulA b0958 0.72 +0.4 1,020,001 - sulA b0958 0.72 +0.1 1,020,042 + sulA b0958 0.80 +0.5 1,020,049 + sulA b0958 0.82 +1.3 1,020,049 + sulA b0958 0.82 +0.7 1,020,226 - +0.3
Or see this region's nucleotide sequence