Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b0943 and ycbF overlap by 8 nucleotides ycbF and pyrD are separated by 110 nucleotides pyrD and ycbW are separated by 173 nucleotides ycbW and ycbX overlap by 4 nucleotides
b0943: b0943 - putative fimbrial-like protein (VIMSS), at 1,002,662 to 1,003,177
b0943
b0944: ycbF - putative chaperone (VIMSS), at 1,003,170 to 1,003,880
ycbF
b0945: pyrD - dihydroorotate dehydrogenase (NCBI), at 1,003,991 to 1,005,001
pyrD
b0946: ycbW - orf, hypothetical protein (VIMSS), at 1,005,175 to 1,005,717
ycbW
b0947: ycbX - predicted 2Fe-2S cluster-containing protein (NCBI), at 1,005,714 to 1,006,823
ycbX
Position (kb)
1003
1004
1005
1006 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1003.071 kb on - strand, within b0943 at 1003.071 kb on - strand, within b0943 at 1003.072 kb on - strand, within b0943 at 1003.109 kb on + strand, within b0943 at 1003.112 kb on + strand, within b0943 at 1003.338 kb on + strand, within ycbF at 1003.385 kb on + strand, within ycbF at 1003.393 kb on - strand, within ycbF at 1003.460 kb on - strand, within ycbF at 1003.616 kb on + strand, within ycbF at 1003.627 kb on + strand, within ycbF at 1003.688 kb on - strand, within ycbF at 1003.705 kb on - strand, within ycbF at 1003.705 kb on - strand, within ycbF at 1003.723 kb on + strand, within ycbF at 1003.723 kb on + strand, within ycbF at 1003.775 kb on + strand, within ycbF at 1003.775 kb on + strand, within ycbF at 1003.959 kb on - strand at 1004.039 kb on - strand at 1004.050 kb on - strand at 1004.119 kb on + strand, within pyrD at 1004.126 kb on + strand, within pyrD at 1004.126 kb on + strand, within pyrD at 1004.126 kb on + strand, within pyrD at 1004.134 kb on + strand, within pyrD at 1004.141 kb on + strand, within pyrD at 1004.174 kb on - strand, within pyrD at 1004.234 kb on + strand, within pyrD at 1004.239 kb on + strand, within pyrD at 1004.256 kb on - strand, within pyrD at 1004.256 kb on - strand, within pyrD at 1004.277 kb on - strand, within pyrD at 1004.307 kb on - strand, within pyrD at 1004.350 kb on + strand, within pyrD at 1004.403 kb on - strand, within pyrD at 1004.440 kb on + strand, within pyrD at 1004.513 kb on + strand, within pyrD at 1004.513 kb on + strand, within pyrD at 1004.523 kb on + strand, within pyrD at 1004.531 kb on - strand, within pyrD at 1004.545 kb on - strand, within pyrD at 1004.553 kb on - strand, within pyrD at 1004.568 kb on + strand, within pyrD at 1004.568 kb on + strand, within pyrD at 1004.614 kb on + strand, within pyrD at 1004.636 kb on + strand, within pyrD at 1004.689 kb on - strand, within pyrD at 1004.698 kb on + strand, within pyrD at 1004.698 kb on + strand, within pyrD at 1004.708 kb on - strand, within pyrD at 1004.717 kb on + strand, within pyrD at 1004.720 kb on - strand, within pyrD at 1004.722 kb on + strand, within pyrD at 1004.727 kb on + strand, within pyrD at 1004.727 kb on + strand, within pyrD at 1004.733 kb on - strand, within pyrD at 1004.733 kb on - strand, within pyrD at 1004.736 kb on - strand, within pyrD at 1004.780 kb on - strand, within pyrD at 1004.783 kb on + strand, within pyrD at 1004.785 kb on + strand, within pyrD at 1004.791 kb on - strand, within pyrD at 1004.791 kb on - strand, within pyrD at 1004.801 kb on - strand, within pyrD at 1004.871 kb on + strand, within pyrD at 1004.871 kb on + strand, within pyrD at 1004.871 kb on + strand, within pyrD at 1004.888 kb on - strand, within pyrD at 1004.938 kb on + strand at 1004.938 kb on + strand at 1005.020 kb on - strand at 1005.067 kb on - strand at 1005.108 kb on - strand at 1005.124 kb on + strand at 1005.137 kb on - strand at 1005.150 kb on + strand at 1005.152 kb on + strand at 1005.160 kb on + strand at 1005.166 kb on - strand at 1005.166 kb on - strand at 1005.168 kb on - strand at 1005.168 kb on - strand at 1005.170 kb on - strand at 1005.215 kb on - strand at 1005.227 kb on + strand at 1005.228 kb on - strand at 1005.228 kb on - strand at 1005.261 kb on - strand, within ycbW at 1005.331 kb on - strand, within ycbW at 1005.405 kb on + strand, within ycbW at 1005.408 kb on + strand, within ycbW at 1005.408 kb on + strand, within ycbW at 1005.413 kb on - strand, within ycbW at 1005.413 kb on - strand, within ycbW at 1005.414 kb on - strand, within ycbW at 1005.414 kb on - strand, within ycbW at 1005.425 kb on - strand, within ycbW at 1005.515 kb on + strand, within ycbW at 1005.522 kb on + strand, within ycbW at 1005.628 kb on + strand, within ycbW at 1005.712 kb on + strand at 1005.720 kb on - strand at 1005.973 kb on - strand, within ycbX at 1005.981 kb on - strand, within ycbX
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 remove 1,003,071 - b0943 b0943 0.79 +0.9 1,003,071 - b0943 b0943 0.79 -0.1 1,003,072 - b0943 b0943 0.79 -0.7 1,003,109 + b0943 b0943 0.87 -0.3 1,003,112 + b0943 b0943 0.87 +0.7 1,003,338 + ycbF b0944 0.24 +0.2 1,003,385 + ycbF b0944 0.30 -0.1 1,003,393 - ycbF b0944 0.31 +0.3 1,003,460 - ycbF b0944 0.41 -0.3 1,003,616 + ycbF b0944 0.63 +0.9 1,003,627 + ycbF b0944 0.64 -0.4 1,003,688 - ycbF b0944 0.73 +1.8 1,003,705 - ycbF b0944 0.75 +0.2 1,003,705 - ycbF b0944 0.75 +0.2 1,003,723 + ycbF b0944 0.78 -0.2 1,003,723 + ycbF b0944 0.78 -1.9 1,003,775 + ycbF b0944 0.85 -1.0 1,003,775 + ycbF b0944 0.85 -0.4 1,003,959 - -0.3 1,004,039 - -0.9 1,004,050 - -0.9 1,004,119 + pyrD b0945 0.13 -0.5 1,004,126 + pyrD b0945 0.13 -0.6 1,004,126 + pyrD b0945 0.13 -2.4 1,004,126 + pyrD b0945 0.13 -2.7 1,004,134 + pyrD b0945 0.14 -3.8 1,004,141 + pyrD b0945 0.15 -0.9 1,004,174 - pyrD b0945 0.18 -2.2 1,004,234 + pyrD b0945 0.24 -1.0 1,004,239 + pyrD b0945 0.25 +0.3 1,004,256 - pyrD b0945 0.26 -0.7 1,004,256 - pyrD b0945 0.26 -0.9 1,004,277 - pyrD b0945 0.28 -0.8 1,004,307 - pyrD b0945 0.31 -1.2 1,004,350 + pyrD b0945 0.36 -2.4 1,004,403 - pyrD b0945 0.41 +0.9 1,004,440 + pyrD b0945 0.44 -0.9 1,004,513 + pyrD b0945 0.52 +2.0 1,004,513 + pyrD b0945 0.52 -3.8 1,004,523 + pyrD b0945 0.53 -0.9 1,004,531 - pyrD b0945 0.53 +2.5 1,004,545 - pyrD b0945 0.55 -1.1 1,004,553 - pyrD b0945 0.56 +1.2 1,004,568 + pyrD b0945 0.57 -0.5 1,004,568 + pyrD b0945 0.57 -0.1 1,004,614 + pyrD b0945 0.62 +0.5 1,004,636 + pyrD b0945 0.64 -1.9 1,004,689 - pyrD b0945 0.69 -1.1 1,004,698 + pyrD b0945 0.70 -1.0 1,004,698 + pyrD b0945 0.70 -0.7 1,004,708 - pyrD b0945 0.71 -2.0 1,004,717 + pyrD b0945 0.72 -0.7 1,004,720 - pyrD b0945 0.72 -2.1 1,004,722 + pyrD b0945 0.72 -0.2 1,004,727 + pyrD b0945 0.73 -3.3 1,004,727 + pyrD b0945 0.73 -0.7 1,004,733 - pyrD b0945 0.73 -2.2 1,004,733 - pyrD b0945 0.73 -0.1 1,004,736 - pyrD b0945 0.74 -0.8 1,004,780 - pyrD b0945 0.78 -0.5 1,004,783 + pyrD b0945 0.78 -0.8 1,004,785 + pyrD b0945 0.79 -0.5 1,004,791 - pyrD b0945 0.79 -1.7 1,004,791 - pyrD b0945 0.79 -0.4 1,004,801 - pyrD b0945 0.80 -0.3 1,004,871 + pyrD b0945 0.87 -0.3 1,004,871 + pyrD b0945 0.87 -2.1 1,004,871 + pyrD b0945 0.87 -1.3 1,004,888 - pyrD b0945 0.89 +0.9 1,004,938 + -0.0 1,004,938 + -1.2 1,005,020 - -0.1 1,005,067 - -0.9 1,005,108 - +0.3 1,005,124 + +0.2 1,005,137 - +0.9 1,005,150 + -1.2 1,005,152 + +0.4 1,005,160 + -0.2 1,005,166 - +0.2 1,005,166 - +0.6 1,005,168 - -0.2 1,005,168 - +0.5 1,005,170 - -0.5 1,005,215 - +0.8 1,005,227 + -1.1 1,005,228 - -0.9 1,005,228 - -0.8 1,005,261 - ycbW b0946 0.16 +0.3 1,005,331 - ycbW b0946 0.29 +0.9 1,005,405 + ycbW b0946 0.42 +1.7 1,005,408 + ycbW b0946 0.43 -1.4 1,005,408 + ycbW b0946 0.43 -1.4 1,005,413 - ycbW b0946 0.44 +1.0 1,005,413 - ycbW b0946 0.44 +0.7 1,005,414 - ycbW b0946 0.44 -1.5 1,005,414 - ycbW b0946 0.44 -0.8 1,005,425 - ycbW b0946 0.46 -1.0 1,005,515 + ycbW b0946 0.63 +0.9 1,005,522 + ycbW b0946 0.64 -0.5 1,005,628 + ycbW b0946 0.83 +0.6 1,005,712 + +0.2 1,005,720 - -0.6 1,005,973 - ycbX b0947 0.23 +0.1 1,005,981 - ycbX b0947 0.24 -0.2
Or see this region's nucleotide sequence