Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0406 and PP_0407 are separated by 1 nucleotides PP_0407 and PP_0408 are separated by 27 nucleotides PP_0408 and PP_0409 are separated by 95 nucleotides
PP_0406: PP_0406 - Nucleotidyltransferase family protein, at 493,567 to 494,238
_0406
PP_0407: PP_0407 - DnaJ-like protein DjlA, at 494,240 to 495,007
_0407
PP_0408: PP_0408 - conserved exported protein of unknown function, at 495,035 to 496,033
_0408
PP_0409: PP_0409 - Sensory box histidine kinase, at 496,129 to 498,516
_0409
Position (kb) 495
496
497 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 494.147 kb on + strand, within PP_0406 at 494.148 kb on - strand, within PP_0406 at 494.161 kb on + strand, within PP_0406 at 494.161 kb on + strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.473 kb on + strand, within PP_0407 at 494.473 kb on + strand, within PP_0407 at 494.626 kb on + strand, within PP_0407 at 494.801 kb on + strand, within PP_0407 at 494.802 kb on - strand, within PP_0407 at 494.802 kb on - strand, within PP_0407 at 494.868 kb on - strand, within PP_0407 at 494.912 kb on - strand, within PP_0407 at 494.912 kb on - strand, within PP_0407 at 494.975 kb on + strand at 494.975 kb on + strand at 494.976 kb on - strand at 494.976 kb on - strand at 495.139 kb on + strand, within PP_0408 at 495.139 kb on + strand, within PP_0408 at 495.207 kb on + strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.214 kb on + strand, within PP_0408 at 495.214 kb on + strand, within PP_0408 at 495.308 kb on + strand, within PP_0408 at 495.336 kb on + strand, within PP_0408 at 495.337 kb on - strand, within PP_0408 at 495.351 kb on + strand, within PP_0408 at 495.361 kb on + strand, within PP_0408 at 495.362 kb on - strand, within PP_0408 at 495.378 kb on - strand, within PP_0408 at 496.068 kb on + strand at 496.069 kb on - strand at 496.074 kb on + strand at 496.075 kb on - strand at 496.075 kb on - strand at 496.126 kb on + strand at 496.126 kb on + strand at 496.126 kb on + strand at 496.127 kb on - strand at 496.127 kb on - strand at 496.291 kb on + strand at 496.324 kb on + strand at 496.351 kb on - strand at 496.351 kb on - strand at 496.389 kb on + strand, within PP_0409 at 496.389 kb on + strand, within PP_0409 at 496.390 kb on - strand, within PP_0409 at 496.390 kb on - strand, within PP_0409 at 496.390 kb on - strand, within PP_0409 at 496.490 kb on + strand at 496.637 kb on - strand, within PP_0409 at 496.694 kb on + strand, within PP_0409 at 496.694 kb on + strand, within PP_0409 at 496.694 kb on + strand, within PP_0409 at 496.694 kb on + strand, within PP_0409 at 496.694 kb on + strand, within PP_0409 at 496.695 kb on - strand, within PP_0409 at 496.695 kb on - strand, within PP_0409 at 496.695 kb on - strand, within PP_0409 at 496.850 kb on + strand, within PP_0409 at 496.924 kb on + strand, within PP_0409
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 494,147 + PP_0406 0.86 +1.6 494,148 - PP_0406 0.86 -0.1 494,161 + PP_0406 0.88 -0.1 494,161 + PP_0406 0.88 +1.1 494,162 - PP_0406 0.89 +0.8 494,162 - PP_0406 0.89 -1.1 494,162 - PP_0406 0.89 -1.8 494,473 + PP_0407 0.30 -1.2 494,473 + PP_0407 0.30 +0.4 494,626 + PP_0407 0.50 +1.5 494,801 + PP_0407 0.73 -0.5 494,802 - PP_0407 0.73 -1.1 494,802 - PP_0407 0.73 +0.8 494,868 - PP_0407 0.82 -0.7 494,912 - PP_0407 0.88 -0.4 494,912 - PP_0407 0.88 +0.6 494,975 + +1.0 494,975 + +0.2 494,976 - -0.9 494,976 - +0.1 495,139 + PP_0408 0.10 -1.3 495,139 + PP_0408 0.10 +1.0 495,207 + PP_0408 0.17 -2.4 495,208 - PP_0408 0.17 +0.9 495,208 - PP_0408 0.17 -0.3 495,208 - PP_0408 0.17 +0.5 495,208 - PP_0408 0.17 +1.0 495,210 + PP_0408 0.18 +1.9 495,210 + PP_0408 0.18 -1.2 495,210 + PP_0408 0.18 -1.3 495,210 + PP_0408 0.18 +1.0 495,210 + PP_0408 0.18 +0.8 495,210 + PP_0408 0.18 -1.6 495,210 + PP_0408 0.18 -1.7 495,210 + PP_0408 0.18 +1.1 495,211 - PP_0408 0.18 -3.1 495,211 - PP_0408 0.18 -2.7 495,211 - PP_0408 0.18 -1.2 495,211 - PP_0408 0.18 +2.4 495,211 - PP_0408 0.18 +0.9 495,214 + PP_0408 0.18 -2.9 495,214 + PP_0408 0.18 +0.7 495,308 + PP_0408 0.27 +0.3 495,336 + PP_0408 0.30 -1.1 495,337 - PP_0408 0.30 +2.6 495,351 + PP_0408 0.32 +1.6 495,361 + PP_0408 0.33 +1.0 495,362 - PP_0408 0.33 +2.1 495,378 - PP_0408 0.34 -0.5 496,068 + -0.4 496,069 - +0.1 496,074 + +1.0 496,075 - -2.2 496,075 - -2.3 496,126 + -0.2 496,126 + -2.2 496,126 + -1.6 496,127 - -0.9 496,127 - +1.6 496,291 + -1.9 496,324 + -2.8 496,351 - +0.6 496,351 - -0.9 496,389 + PP_0409 0.11 +1.2 496,389 + PP_0409 0.11 +1.8 496,390 - PP_0409 0.11 +0.1 496,390 - PP_0409 0.11 -0.6 496,390 - PP_0409 0.11 -0.0 496,490 + +1.2 496,637 - PP_0409 0.21 +1.1 496,694 + PP_0409 0.24 -1.3 496,694 + PP_0409 0.24 +0.0 496,694 + PP_0409 0.24 +4.3 496,694 + PP_0409 0.24 +0.2 496,694 + PP_0409 0.24 +0.7 496,695 - PP_0409 0.24 -2.1 496,695 - PP_0409 0.24 +1.2 496,695 - PP_0409 0.24 -1.3 496,850 + PP_0409 0.30 -0.4 496,924 + PP_0409 0.33 -2.3
Or see this region's nucleotide sequence