Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5421 and PP_0014 overlap by 20 nucleotides PP_0014 and PP_0015 overlap by 4 nucleotides
PP_5421: PP_5421 - conserved protein of unknown function, at 16,589 to 17,227
_5421
PP_0014: PP_0014 - putative transposase, at 17,208 to 19,166
_0014
PP_0015: PP_0015 - putative transposon protein, at 19,163 to 20,122
_0015
Position (kb) 17
18
19
20 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 16.270 kb on - strand at 16.389 kb on + strand at 16.389 kb on + strand at 16.472 kb on + strand at 16.597 kb on - strand at 16.781 kb on + strand, within PP_5421 at 16.781 kb on + strand, within PP_5421 at 16.791 kb on - strand, within PP_5421 at 16.791 kb on - strand, within PP_5421 at 16.791 kb on - strand, within PP_5421 at 16.804 kb on - strand, within PP_5421 at 16.804 kb on - strand, within PP_5421 at 16.907 kb on + strand, within PP_5421 at 16.984 kb on + strand, within PP_5421 at 16.984 kb on + strand, within PP_5421 at 16.984 kb on + strand, within PP_5421 at 16.984 kb on + strand, within PP_5421 at 16.984 kb on + strand, within PP_5421 at 16.984 kb on + strand, within PP_5421 at 16.985 kb on - strand, within PP_5421 at 16.985 kb on - strand, within PP_5421 at 16.997 kb on + strand, within PP_5421 at 16.997 kb on + strand, within PP_5421 at 16.997 kb on + strand, within PP_5421 at 17.043 kb on + strand, within PP_5421 at 17.044 kb on - strand, within PP_5421 at 17.044 kb on - strand, within PP_5421 at 17.044 kb on - strand, within PP_5421 at 17.044 kb on - strand, within PP_5421 at 17.044 kb on - strand, within PP_5421 at 17.071 kb on + strand, within PP_5421 at 17.093 kb on + strand, within PP_5421 at 17.093 kb on + strand, within PP_5421 at 17.093 kb on + strand, within PP_5421 at 17.094 kb on - strand, within PP_5421 at 17.094 kb on - strand, within PP_5421 at 17.127 kb on + strand, within PP_5421 at 17.158 kb on + strand, within PP_5421 at 17.158 kb on + strand, within PP_5421 at 17.158 kb on + strand, within PP_5421 at 17.159 kb on - strand, within PP_5421 at 17.159 kb on - strand, within PP_5421 at 17.159 kb on - strand, within PP_5421 at 17.159 kb on - strand, within PP_5421 at 17.257 kb on + strand at 17.257 kb on + strand at 17.283 kb on - strand at 17.283 kb on - strand at 17.283 kb on - strand at 17.349 kb on + strand at 17.349 kb on + strand at 17.349 kb on + strand at 17.349 kb on + strand at 17.349 kb on + strand at 17.358 kb on + strand at 17.358 kb on + strand at 17.358 kb on + strand at 17.359 kb on - strand at 17.397 kb on + strand at 17.415 kb on + strand, within PP_0014 at 17.415 kb on + strand, within PP_0014 at 17.415 kb on + strand, within PP_0014 at 17.416 kb on - strand, within PP_0014 at 17.416 kb on - strand, within PP_0014 at 17.504 kb on - strand, within PP_0014 at 17.504 kb on - strand, within PP_0014 at 17.508 kb on - strand, within PP_0014 at 17.521 kb on - strand, within PP_0014 at 17.528 kb on - strand, within PP_0014 at 17.558 kb on + strand, within PP_0014 at 17.559 kb on - strand, within PP_0014 at 17.565 kb on - strand, within PP_0014 at 17.647 kb on + strand, within PP_0014 at 17.647 kb on + strand, within PP_0014 at 17.647 kb on + strand, within PP_0014 at 17.647 kb on + strand, within PP_0014 at 17.650 kb on + strand, within PP_0014 at 17.650 kb on + strand, within PP_0014 at 17.650 kb on + strand, within PP_0014 at 17.651 kb on - strand, within PP_0014 at 17.658 kb on - strand, within PP_0014 at 17.658 kb on - strand, within PP_0014 at 17.733 kb on - strand, within PP_0014 at 17.735 kb on + strand, within PP_0014 at 17.736 kb on - strand, within PP_0014 at 17.743 kb on - strand, within PP_0014 at 17.872 kb on - strand, within PP_0014 at 17.893 kb on - strand, within PP_0014 at 17.962 kb on + strand, within PP_0014 at 17.962 kb on + strand, within PP_0014 at 17.962 kb on + strand, within PP_0014 at 17.962 kb on + strand, within PP_0014 at 17.962 kb on + strand, within PP_0014 at 17.962 kb on + strand, within PP_0014 at 17.963 kb on - strand, within PP_0014 at 18.105 kb on + strand, within PP_0014 at 18.106 kb on - strand, within PP_0014 at 18.106 kb on - strand, within PP_0014 at 18.106 kb on - strand, within PP_0014 at 18.132 kb on - strand, within PP_0014 at 18.133 kb on - strand, within PP_0014 at 18.244 kb on + strand, within PP_0014 at 18.244 kb on + strand, within PP_0014 at 18.245 kb on - strand, within PP_0014 at 18.321 kb on + strand, within PP_0014 at 18.322 kb on - strand, within PP_0014 at 18.322 kb on - strand, within PP_0014 at 18.392 kb on + strand, within PP_0014 at 18.578 kb on + strand, within PP_0014 at 18.578 kb on + strand, within PP_0014 at 18.579 kb on - strand, within PP_0014 at 18.704 kb on + strand, within PP_0014 at 18.725 kb on + strand, within PP_0014 at 18.751 kb on + strand, within PP_0014 at 18.752 kb on - strand, within PP_0014 at 18.901 kb on - strand, within PP_0014 at 18.902 kb on + strand, within PP_0014 at 18.902 kb on + strand, within PP_0014 at 18.914 kb on + strand, within PP_0014 at 18.915 kb on - strand, within PP_0014 at 18.915 kb on - strand, within PP_0014 at 18.915 kb on - strand, within PP_0014 at 18.926 kb on + strand, within PP_0014 at 18.926 kb on + strand, within PP_0014 at 18.939 kb on + strand, within PP_0014 at 18.940 kb on - strand, within PP_0014 at 18.950 kb on + strand, within PP_0014 at 18.951 kb on - strand, within PP_0014 at 19.066 kb on + strand at 19.066 kb on + strand at 19.066 kb on + strand at 19.067 kb on - strand at 19.097 kb on + strand at 19.097 kb on + strand at 19.097 kb on + strand at 19.097 kb on + strand at 19.098 kb on - strand at 19.098 kb on - strand at 19.098 kb on - strand at 19.098 kb on - strand at 19.103 kb on + strand at 19.104 kb on - strand at 19.157 kb on + strand at 19.157 kb on + strand at 19.158 kb on - strand at 19.158 kb on - strand at 19.158 kb on - strand at 19.181 kb on + strand at 19.205 kb on + strand at 19.205 kb on + strand at 19.206 kb on - strand at 19.206 kb on - strand at 19.206 kb on - strand at 19.213 kb on + strand at 19.213 kb on + strand at 19.213 kb on + strand at 19.213 kb on + strand at 19.213 kb on + strand at 19.214 kb on - strand at 19.231 kb on + strand at 19.232 kb on - strand at 19.269 kb on + strand, within PP_0015 at 19.270 kb on - strand, within PP_0015 at 19.309 kb on + strand, within PP_0015 at 19.309 kb on + strand, within PP_0015 at 19.310 kb on - strand, within PP_0015 at 19.367 kb on + strand, within PP_0015 at 19.367 kb on + strand, within PP_0015 at 19.367 kb on + strand, within PP_0015 at 19.368 kb on - strand, within PP_0015 at 19.368 kb on - strand, within PP_0015 at 19.529 kb on - strand, within PP_0015 at 19.529 kb on - strand, within PP_0015 at 19.542 kb on + strand, within PP_0015 at 19.543 kb on - strand, within PP_0015 at 19.610 kb on + strand, within PP_0015 at 19.618 kb on + strand, within PP_0015 at 19.618 kb on + strand, within PP_0015 at 19.618 kb on + strand, within PP_0015 at 19.619 kb on - strand, within PP_0015 at 19.619 kb on - strand, within PP_0015 at 19.619 kb on - strand, within PP_0015 at 19.628 kb on + strand, within PP_0015 at 19.628 kb on + strand, within PP_0015 at 19.629 kb on - strand, within PP_0015 at 19.629 kb on - strand, within PP_0015 at 19.629 kb on - strand, within PP_0015 at 19.629 kb on - strand, within PP_0015 at 19.629 kb on - strand, within PP_0015 at 19.672 kb on + strand, within PP_0015 at 19.720 kb on - strand, within PP_0015 at 19.789 kb on + strand, within PP_0015 at 19.789 kb on + strand, within PP_0015 at 19.789 kb on + strand, within PP_0015 at 19.790 kb on - strand, within PP_0015 at 19.790 kb on - strand, within PP_0015 at 19.790 kb on - strand, within PP_0015 at 19.790 kb on - strand, within PP_0015 at 19.860 kb on + strand, within PP_0015 at 19.876 kb on + strand, within PP_0015 at 19.877 kb on - strand, within PP_0015 at 19.877 kb on - strand, within PP_0015 at 19.886 kb on + strand, within PP_0015 at 19.886 kb on + strand, within PP_0015 at 19.886 kb on + strand, within PP_0015 at 19.887 kb on - strand, within PP_0015 at 19.898 kb on + strand, within PP_0015 at 19.913 kb on + strand, within PP_0015 at 19.913 kb on + strand, within PP_0015 at 19.914 kb on - strand at 20.021 kb on + strand, within PP_0015 at 20.021 kb on + strand, within PP_0015 at 20.022 kb on - strand, within PP_0015 at 20.022 kb on - strand, within PP_0015 at 20.022 kb on - strand, within PP_0015 at 20.022 kb on - strand, within PP_0015 at 20.116 kb on + strand at 20.116 kb on + strand at 20.117 kb on - strand at 20.117 kb on - strand at 20.117 kb on - strand at 20.117 kb on - strand at 20.154 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 16,270 - +1.1 16,389 + +1.6 16,389 + +1.0 16,472 + -0.4 16,597 - +0.8 16,781 + PP_5421 0.30 -0.3 16,781 + PP_5421 0.30 -0.9 16,791 - PP_5421 0.32 -1.1 16,791 - PP_5421 0.32 -1.7 16,791 - PP_5421 0.32 -0.2 16,804 - PP_5421 0.34 -2.1 16,804 - PP_5421 0.34 +1.4 16,907 + PP_5421 0.50 -1.2 16,984 + PP_5421 0.62 -0.8 16,984 + PP_5421 0.62 +0.9 16,984 + PP_5421 0.62 -1.2 16,984 + PP_5421 0.62 -1.1 16,984 + PP_5421 0.62 +0.9 16,984 + PP_5421 0.62 -2.0 16,985 - PP_5421 0.62 -1.2 16,985 - PP_5421 0.62 +0.5 16,997 + PP_5421 0.64 +0.8 16,997 + PP_5421 0.64 -1.6 16,997 + PP_5421 0.64 +0.5 17,043 + PP_5421 0.71 -1.7 17,044 - PP_5421 0.71 -1.3 17,044 - PP_5421 0.71 +0.8 17,044 - PP_5421 0.71 +1.1 17,044 - PP_5421 0.71 +0.4 17,044 - PP_5421 0.71 +1.1 17,071 + PP_5421 0.75 +2.0 17,093 + PP_5421 0.79 -2.4 17,093 + PP_5421 0.79 +0.1 17,093 + PP_5421 0.79 +0.5 17,094 - PP_5421 0.79 +0.2 17,094 - PP_5421 0.79 -1.0 17,127 + PP_5421 0.84 -1.9 17,158 + PP_5421 0.89 +1.3 17,158 + PP_5421 0.89 +0.2 17,158 + PP_5421 0.89 -1.1 17,159 - PP_5421 0.89 -1.8 17,159 - PP_5421 0.89 +1.7 17,159 - PP_5421 0.89 -2.1 17,159 - PP_5421 0.89 -1.6 17,257 + +1.0 17,257 + +0.1 17,283 - -1.9 17,283 - -2.5 17,283 - +0.1 17,349 + -1.0 17,349 + +0.6 17,349 + +1.4 17,349 + -0.2 17,349 + -0.2 17,358 + +1.2 17,358 + -0.2 17,358 + +1.4 17,359 - +2.4 17,397 + +1.5 17,415 + PP_0014 0.11 -2.5 17,415 + PP_0014 0.11 +0.8 17,415 + PP_0014 0.11 -0.8 17,416 - PP_0014 0.11 -1.4 17,416 - PP_0014 0.11 -0.6 17,504 - PP_0014 0.15 +0.9 17,504 - PP_0014 0.15 +2.6 17,508 - PP_0014 0.15 +0.6 17,521 - PP_0014 0.16 -1.6 17,528 - PP_0014 0.16 -0.2 17,558 + PP_0014 0.18 -0.2 17,559 - PP_0014 0.18 +0.8 17,565 - PP_0014 0.18 -0.1 17,647 + PP_0014 0.22 -2.5 17,647 + PP_0014 0.22 +2.4 17,647 + PP_0014 0.22 +0.6 17,647 + PP_0014 0.22 +1.8 17,650 + PP_0014 0.23 +1.4 17,650 + PP_0014 0.23 +0.5 17,650 + PP_0014 0.23 -1.5 17,651 - PP_0014 0.23 -0.3 17,658 - PP_0014 0.23 -1.5 17,658 - PP_0014 0.23 +0.5 17,733 - PP_0014 0.27 -0.3 17,735 + PP_0014 0.27 +2.3 17,736 - PP_0014 0.27 -0.4 17,743 - PP_0014 0.27 +1.2 17,872 - PP_0014 0.34 -2.6 17,893 - PP_0014 0.35 +0.1 17,962 + PP_0014 0.38 +0.2 17,962 + PP_0014 0.38 +2.1 17,962 + PP_0014 0.38 +0.6 17,962 + PP_0014 0.38 -0.5 17,962 + PP_0014 0.38 -1.1 17,962 + PP_0014 0.38 +1.2 17,963 - PP_0014 0.39 -1.9 18,105 + PP_0014 0.46 +1.9 18,106 - PP_0014 0.46 -0.4 18,106 - PP_0014 0.46 +2.0 18,106 - PP_0014 0.46 +1.1 18,132 - PP_0014 0.47 +2.4 18,133 - PP_0014 0.47 +0.2 18,244 + PP_0014 0.53 -0.5 18,244 + PP_0014 0.53 -1.2 18,245 - PP_0014 0.53 -1.5 18,321 + PP_0014 0.57 +1.9 18,322 - PP_0014 0.57 +0.4 18,322 - PP_0014 0.57 +0.1 18,392 + PP_0014 0.60 -1.4 18,578 + PP_0014 0.70 +0.3 18,578 + PP_0014 0.70 +1.4 18,579 - PP_0014 0.70 +0.2 18,704 + PP_0014 0.76 -0.5 18,725 + PP_0014 0.77 -1.7 18,751 + PP_0014 0.79 +1.1 18,752 - PP_0014 0.79 +0.8 18,901 - PP_0014 0.86 -1.7 18,902 + PP_0014 0.86 -0.4 18,902 + PP_0014 0.86 +1.4 18,914 + PP_0014 0.87 -1.8 18,915 - PP_0014 0.87 -1.0 18,915 - PP_0014 0.87 +0.9 18,915 - PP_0014 0.87 +0.7 18,926 + PP_0014 0.88 -0.6 18,926 + PP_0014 0.88 -0.7 18,939 + PP_0014 0.88 +1.2 18,940 - PP_0014 0.88 -2.5 18,950 + PP_0014 0.89 +0.2 18,951 - PP_0014 0.89 -0.3 19,066 + -1.9 19,066 + +0.2 19,066 + -1.1 19,067 - -0.3 19,097 + +0.5 19,097 + +1.0 19,097 + +0.4 19,097 + -2.2 19,098 - +0.9 19,098 - +0.4 19,098 - -0.1 19,098 - +0.2 19,103 + +1.2 19,104 - +0.4 19,157 + +0.8 19,157 + +2.9 19,158 - -0.2 19,158 - -2.7 19,158 - -1.2 19,181 + +1.2 19,205 + +1.1 19,205 + -0.4 19,206 - -2.7 19,206 - -0.4 19,206 - -1.8 19,213 + +0.3 19,213 + +2.2 19,213 + -1.6 19,213 + -1.7 19,213 + +0.1 19,214 - -2.7 19,231 + +1.9 19,232 - +1.2 19,269 + PP_0015 0.11 +0.9 19,270 - PP_0015 0.11 +0.3 19,309 + PP_0015 0.15 -0.1 19,309 + PP_0015 0.15 +0.3 19,310 - PP_0015 0.15 -0.1 19,367 + PP_0015 0.21 -0.6 19,367 + PP_0015 0.21 -0.9 19,367 + PP_0015 0.21 -1.2 19,368 - PP_0015 0.21 +0.2 19,368 - PP_0015 0.21 +0.8 19,529 - PP_0015 0.38 -2.3 19,529 - PP_0015 0.38 -0.4 19,542 + PP_0015 0.39 +0.9 19,543 - PP_0015 0.40 -1.4 19,610 + PP_0015 0.47 -0.5 19,618 + PP_0015 0.47 +2.4 19,618 + PP_0015 0.47 +1.0 19,618 + PP_0015 0.47 -0.2 19,619 - PP_0015 0.47 +1.9 19,619 - PP_0015 0.47 +0.0 19,619 - PP_0015 0.47 +0.2 19,628 + PP_0015 0.48 -0.4 19,628 + PP_0015 0.48 +0.1 19,629 - PP_0015 0.49 -2.4 19,629 - PP_0015 0.49 +0.8 19,629 - PP_0015 0.49 -1.3 19,629 - PP_0015 0.49 -1.6 19,629 - PP_0015 0.49 -0.4 19,672 + PP_0015 0.53 -1.7 19,720 - PP_0015 0.58 +2.2 19,789 + PP_0015 0.65 -0.2 19,789 + PP_0015 0.65 -1.6 19,789 + PP_0015 0.65 +0.4 19,790 - PP_0015 0.65 +1.3 19,790 - PP_0015 0.65 +0.9 19,790 - PP_0015 0.65 -2.2 19,790 - PP_0015 0.65 -0.5 19,860 + PP_0015 0.73 -0.7 19,876 + PP_0015 0.74 +0.7 19,877 - PP_0015 0.74 -2.0 19,877 - PP_0015 0.74 +1.1 19,886 + PP_0015 0.75 +0.8 19,886 + PP_0015 0.75 -1.0 19,886 + PP_0015 0.75 -1.0 19,887 - PP_0015 0.75 +0.3 19,898 + PP_0015 0.77 -0.0 19,913 + PP_0015 0.78 +2.0 19,913 + PP_0015 0.78 -0.8 19,914 - +0.3 20,021 + PP_0015 0.89 +0.5 20,021 + PP_0015 0.89 +1.6 20,022 - PP_0015 0.89 -2.3 20,022 - PP_0015 0.89 -1.1 20,022 - PP_0015 0.89 -0.5 20,022 - PP_0015 0.89 +1.5 20,116 + -2.1 20,116 + +0.6 20,117 - -2.2 20,117 - +0.8 20,117 - -0.5 20,117 - -0.4 20,154 + +1.9
Or see this region's nucleotide sequence