Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0124 and PP_0125 are separated by 201 nucleotides PP_0125 and PP_0126 are separated by 31 nucleotides PP_0126 and PP_0127 are separated by 151 nucleotides PP_0127 and PP_0128 are separated by 12 nucleotides
PP_0124: PP_0124 - probable GTP-binding protein, at 130,641 to 131,273
_0124
PP_0125: PP_0125 - putative cytochrome c-type protein, at 131,475 to 131,777
_0125
PP_0126: PP_0126 - Cytochrome c4, at 131,809 to 132,426
_0126
PP_0127: PP_0127 - Thiol:disulfide interchange protein, at 132,578 to 133,210
_0127
PP_0128: PP_0128 - conserved protein of unknown function, at 133,223 to 134,077
_0128
Position (kb)
131
132
133 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 131.355 kb on - strand at 131.355 kb on - strand at 131.356 kb on + strand at 131.356 kb on + strand at 131.356 kb on + strand at 131.357 kb on - strand at 131.357 kb on - strand at 131.357 kb on - strand at 131.357 kb on - strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.424 kb on - strand at 131.424 kb on - strand at 131.424 kb on - strand at 131.502 kb on - strand at 131.502 kb on - strand at 131.502 kb on - strand at 131.532 kb on - strand, within PP_0125 at 131.532 kb on - strand, within PP_0125 at 131.553 kb on + strand, within PP_0125 at 131.554 kb on - strand, within PP_0125 at 131.554 kb on - strand, within PP_0125 at 131.656 kb on + strand, within PP_0125 at 131.772 kb on - strand at 131.775 kb on + strand at 131.775 kb on + strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.789 kb on + strand at 131.789 kb on + strand at 131.789 kb on + strand at 131.790 kb on - strand at 131.803 kb on + strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.819 kb on + strand at 131.819 kb on + strand at 131.819 kb on + strand at 131.820 kb on - strand at 131.820 kb on - strand at 131.877 kb on + strand, within PP_0126 at 131.877 kb on + strand, within PP_0126 at 131.995 kb on + strand, within PP_0126 at 132.019 kb on - strand, within PP_0126 at 132.036 kb on + strand, within PP_0126 at 132.037 kb on - strand, within PP_0126 at 132.103 kb on + strand, within PP_0126 at 132.286 kb on + strand, within PP_0126 at 132.406 kb on + strand at 132.406 kb on + strand at 132.407 kb on - strand at 132.424 kb on + strand at 132.443 kb on + strand at 132.443 kb on + strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.572 kb on + strand at 132.572 kb on + strand at 132.572 kb on + strand at 132.573 kb on - strand at 132.583 kb on + strand at 132.584 kb on - strand at 132.584 kb on - strand at 132.584 kb on - strand at 132.671 kb on + strand, within PP_0127 at 132.671 kb on + strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.740 kb on + strand, within PP_0127 at 133.080 kb on - strand, within PP_0127 at 133.080 kb on - strand, within PP_0127 at 133.096 kb on - strand, within PP_0127 at 133.122 kb on - strand, within PP_0127 at 133.208 kb on + strand at 133.208 kb on + strand at 133.208 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.241 kb on - strand at 133.387 kb on + strand, within PP_0128 at 133.387 kb on + strand, within PP_0128 at 133.388 kb on - strand, within PP_0128
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 131,355 - -0.7 131,355 - -0.9 131,356 + -2.1 131,356 + +0.8 131,356 + +0.6 131,357 - +0.1 131,357 - +1.1 131,357 - -0.6 131,357 - -0.0 131,358 + +0.3 131,358 + -1.8 131,358 + -0.6 131,358 + -1.1 131,358 + -2.2 131,358 + +0.6 131,358 + +0.4 131,358 + -1.3 131,358 + +0.3 131,359 - -0.3 131,359 - -0.5 131,359 - +1.5 131,359 - -2.9 131,359 - +1.3 131,359 - -2.1 131,359 - -1.2 131,359 - -0.0 131,359 - -1.0 131,424 - +0.5 131,424 - +0.5 131,424 - -0.7 131,502 - -1.0 131,502 - +0.7 131,502 - -0.8 131,532 - PP_0125 0.19 -0.3 131,532 - PP_0125 0.19 +0.6 131,553 + PP_0125 0.26 -2.3 131,554 - PP_0125 0.26 -1.4 131,554 - PP_0125 0.26 +0.4 131,656 + PP_0125 0.60 +0.0 131,772 - -1.2 131,775 + +0.8 131,775 + -0.5 131,776 - +1.4 131,776 - +1.0 131,776 - -2.2 131,776 - +0.2 131,789 + -1.0 131,789 + -3.1 131,789 + +2.2 131,790 - -0.7 131,803 + -2.6 131,804 - -1.5 131,804 - +0.0 131,804 - -1.3 131,804 - -0.9 131,819 + -1.7 131,819 + +0.9 131,819 + +1.2 131,820 - -0.6 131,820 - +0.9 131,877 + PP_0126 0.11 +0.9 131,877 + PP_0126 0.11 +0.0 131,995 + PP_0126 0.30 +1.2 132,019 - PP_0126 0.34 +1.7 132,036 + PP_0126 0.37 -0.1 132,037 - PP_0126 0.37 +0.8 132,103 + PP_0126 0.48 +0.2 132,286 + PP_0126 0.77 -0.0 132,406 + -0.8 132,406 + -0.8 132,407 - -1.0 132,424 + -0.3 132,443 + +0.9 132,443 + -0.5 132,444 - +0.9 132,444 - +0.1 132,444 - +0.7 132,444 - -2.5 132,502 - +1.1 132,502 - +2.1 132,502 - -0.1 132,502 - -1.8 132,572 + -1.8 132,572 + +1.0 132,572 + +1.0 132,573 - -1.7 132,583 + +0.8 132,584 - +1.4 132,584 - -2.0 132,584 - -1.7 132,671 + PP_0127 0.15 -1.4 132,671 + PP_0127 0.15 -0.7 132,672 - PP_0127 0.15 +0.6 132,672 - PP_0127 0.15 +0.3 132,672 - PP_0127 0.15 -0.7 132,740 + PP_0127 0.26 +0.3 133,080 - PP_0127 0.79 -0.5 133,080 - PP_0127 0.79 -0.2 133,096 - PP_0127 0.82 -0.3 133,122 - PP_0127 0.86 -0.3 133,208 + -2.2 133,208 + -0.9 133,208 + +0.7 133,240 + +0.4 133,240 + +1.9 133,240 + +0.4 133,240 + -0.2 133,240 + +0.6 133,241 - -0.9 133,387 + PP_0128 0.19 +1.7 133,387 + PP_0128 0.19 -1.3 133,388 - PP_0128 0.19 +0.2
Or see this region's nucleotide sequence