Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2030 and PP_2031 overlap by 4 nucleotides PP_2031 and PP_2032 overlap by 8 nucleotides PP_2032 and PP_2033 are separated by 228 nucleotides
PP_2030: PP_2030 - conserved protein of unknown function, at 2,307,622 to 2,308,116
_2030
PP_2031: PP_2031 - conserved protein of unknown function, at 2,308,113 to 2,309,069
_2031
PP_2032: PP_2032 - putative ATPase, at 2,309,062 to 2,310,021
_2032
PP_2033: PP_2033 - conserved protein of unknown function, at 2,310,250 to 2,311,560
_2033
Position (kb)
2309
2310
2311 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2308.827 kb on - strand, within PP_2031 at 2308.877 kb on - strand, within PP_2031 at 2308.877 kb on - strand, within PP_2031 at 2309.348 kb on + strand, within PP_2032 at 2309.348 kb on + strand, within PP_2032 at 2309.372 kb on + strand, within PP_2032 at 2309.372 kb on + strand, within PP_2032 at 2309.372 kb on + strand, within PP_2032 at 2309.373 kb on - strand, within PP_2032 at 2309.373 kb on - strand, within PP_2032 at 2309.488 kb on + strand, within PP_2032 at 2309.516 kb on - strand at 2309.558 kb on + strand, within PP_2032 at 2309.559 kb on - strand, within PP_2032 at 2309.621 kb on + strand, within PP_2032 at 2309.622 kb on - strand, within PP_2032 at 2309.622 kb on - strand, within PP_2032 at 2309.622 kb on - strand, within PP_2032 at 2309.725 kb on - strand, within PP_2032 at 2309.733 kb on + strand, within PP_2032 at 2309.733 kb on + strand, within PP_2032 at 2309.734 kb on - strand, within PP_2032 at 2309.965 kb on + strand at 2309.965 kb on + strand at 2309.965 kb on + strand at 2309.977 kb on - strand at 2310.085 kb on - strand at 2310.085 kb on - strand at 2310.089 kb on + strand at 2310.089 kb on + strand at 2310.090 kb on - strand at 2310.090 kb on - strand at 2310.090 kb on - strand at 2310.090 kb on - strand at 2310.132 kb on + strand at 2310.286 kb on - strand at 2310.385 kb on + strand, within PP_2033 at 2310.386 kb on - strand, within PP_2033 at 2310.386 kb on - strand, within PP_2033 at 2310.428 kb on - strand, within PP_2033 at 2310.428 kb on - strand, within PP_2033 at 2310.428 kb on - strand, within PP_2033 at 2310.478 kb on + strand, within PP_2033 at 2310.478 kb on + strand, within PP_2033 at 2310.478 kb on + strand, within PP_2033 at 2310.479 kb on - strand, within PP_2033 at 2310.524 kb on - strand, within PP_2033 at 2310.524 kb on - strand, within PP_2033 at 2310.524 kb on - strand, within PP_2033 at 2310.806 kb on + strand, within PP_2033 at 2310.806 kb on + strand, within PP_2033 at 2310.807 kb on - strand, within PP_2033 at 2310.918 kb on + strand, within PP_2033 at 2310.919 kb on - strand, within PP_2033 at 2310.975 kb on + strand, within PP_2033
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 2,308,827 - PP_2031 0.75 +0.8 2,308,877 - PP_2031 0.80 +1.7 2,308,877 - PP_2031 0.80 +0.5 2,309,348 + PP_2032 0.30 +0.8 2,309,348 + PP_2032 0.30 +1.6 2,309,372 + PP_2032 0.32 +1.4 2,309,372 + PP_2032 0.32 +2.1 2,309,372 + PP_2032 0.32 +0.8 2,309,373 - PP_2032 0.32 -0.7 2,309,373 - PP_2032 0.32 +2.1 2,309,488 + PP_2032 0.44 +1.6 2,309,516 - +1.4 2,309,558 + PP_2032 0.52 +0.3 2,309,559 - PP_2032 0.52 +1.5 2,309,621 + PP_2032 0.58 +0.6 2,309,622 - PP_2032 0.58 +0.6 2,309,622 - PP_2032 0.58 +1.0 2,309,622 - PP_2032 0.58 +1.6 2,309,725 - PP_2032 0.69 +2.8 2,309,733 + PP_2032 0.70 +0.3 2,309,733 + PP_2032 0.70 +1.5 2,309,734 - PP_2032 0.70 +0.6 2,309,965 + +1.5 2,309,965 + +2.3 2,309,965 + +1.7 2,309,977 - -0.1 2,310,085 - -1.2 2,310,085 - -0.1 2,310,089 + +1.3 2,310,089 + +1.3 2,310,090 - -2.8 2,310,090 - -0.1 2,310,090 - -0.3 2,310,090 - +0.4 2,310,132 + +1.4 2,310,286 - -0.2 2,310,385 + PP_2033 0.10 +0.5 2,310,386 - PP_2033 0.10 -0.0 2,310,386 - PP_2033 0.10 +0.4 2,310,428 - PP_2033 0.14 -1.7 2,310,428 - PP_2033 0.14 -0.7 2,310,428 - PP_2033 0.14 +0.5 2,310,478 + PP_2033 0.17 +0.5 2,310,478 + PP_2033 0.17 -0.6 2,310,478 + PP_2033 0.17 -0.6 2,310,479 - PP_2033 0.17 -0.1 2,310,524 - PP_2033 0.21 -1.5 2,310,524 - PP_2033 0.21 +0.3 2,310,524 - PP_2033 0.21 +0.6 2,310,806 + PP_2033 0.42 -0.4 2,310,806 + PP_2033 0.42 -1.0 2,310,807 - PP_2033 0.42 +0.2 2,310,918 + PP_2033 0.51 -0.9 2,310,919 - PP_2033 0.51 -0.0 2,310,975 + PP_2033 0.55 +0.1
Or see this region's nucleotide sequence