Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0125 and PP_0126 are separated by 31 nucleotides PP_0126 and PP_0127 are separated by 151 nucleotides PP_0127 and PP_0128 are separated by 12 nucleotides PP_0128 and PP_0129 are separated by 27 nucleotides
PP_0125: PP_0125 - putative cytochrome c-type protein, at 131,475 to 131,777
_0125
PP_0126: PP_0126 - Cytochrome c4, at 131,809 to 132,426
_0126
PP_0127: PP_0127 - Thiol:disulfide interchange protein, at 132,578 to 133,210
_0127
PP_0128: PP_0128 - conserved protein of unknown function, at 133,223 to 134,077
_0128
PP_0129: PP_0129 - GGDEF domain protein, at 134,105 to 136,042
_0129
Position (kb) 132
133
134 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 131.656 kb on + strand, within PP_0125 at 131.772 kb on - strand at 131.775 kb on + strand at 131.775 kb on + strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.789 kb on + strand at 131.789 kb on + strand at 131.789 kb on + strand at 131.790 kb on - strand at 131.803 kb on + strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.819 kb on + strand at 131.819 kb on + strand at 131.819 kb on + strand at 131.820 kb on - strand at 131.820 kb on - strand at 131.877 kb on + strand, within PP_0126 at 131.877 kb on + strand, within PP_0126 at 131.995 kb on + strand, within PP_0126 at 132.019 kb on - strand, within PP_0126 at 132.036 kb on + strand, within PP_0126 at 132.037 kb on - strand, within PP_0126 at 132.103 kb on + strand, within PP_0126 at 132.286 kb on + strand, within PP_0126 at 132.406 kb on + strand at 132.406 kb on + strand at 132.407 kb on - strand at 132.424 kb on + strand at 132.443 kb on + strand at 132.443 kb on + strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.572 kb on + strand at 132.572 kb on + strand at 132.572 kb on + strand at 132.573 kb on - strand at 132.583 kb on + strand at 132.584 kb on - strand at 132.584 kb on - strand at 132.584 kb on - strand at 132.671 kb on + strand, within PP_0127 at 132.671 kb on + strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.740 kb on + strand, within PP_0127 at 133.080 kb on - strand, within PP_0127 at 133.080 kb on - strand, within PP_0127 at 133.096 kb on - strand, within PP_0127 at 133.122 kb on - strand, within PP_0127 at 133.208 kb on + strand at 133.208 kb on + strand at 133.208 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.241 kb on - strand at 133.387 kb on + strand, within PP_0128 at 133.387 kb on + strand, within PP_0128 at 133.388 kb on - strand, within PP_0128 at 133.511 kb on + strand, within PP_0128 at 133.512 kb on - strand, within PP_0128 at 133.562 kb on + strand, within PP_0128 at 133.562 kb on + strand, within PP_0128 at 133.563 kb on - strand, within PP_0128 at 133.720 kb on - strand, within PP_0128 at 133.775 kb on + strand, within PP_0128 at 133.775 kb on + strand, within PP_0128 at 133.775 kb on + strand, within PP_0128 at 133.776 kb on - strand, within PP_0128 at 133.777 kb on + strand, within PP_0128 at 133.778 kb on - strand, within PP_0128 at 133.800 kb on - strand, within PP_0128 at 133.942 kb on + strand, within PP_0128 at 133.942 kb on + strand, within PP_0128 at 133.943 kb on - strand, within PP_0128 at 133.943 kb on - strand, within PP_0128 at 133.943 kb on - strand, within PP_0128 at 133.943 kb on - strand, within PP_0128 at 134.056 kb on + strand at 134.056 kb on + strand at 134.056 kb on + strand at 134.075 kb on + strand at 134.076 kb on - strand at 134.076 kb on - strand at 134.139 kb on - strand at 134.143 kb on + strand at 134.143 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 131,656 + PP_0125 0.60 -0.9 131,772 - -1.4 131,775 + +1.8 131,775 + +0.4 131,776 - +0.4 131,776 - +0.9 131,776 - -0.0 131,776 - -1.4 131,789 + +0.0 131,789 + +1.2 131,789 + +0.5 131,790 - +0.1 131,803 + -2.8 131,804 - -0.1 131,804 - +1.8 131,804 - -0.9 131,804 - -1.1 131,819 + +0.4 131,819 + -1.2 131,819 + +0.0 131,820 - -1.8 131,820 - -1.6 131,877 + PP_0126 0.11 -0.5 131,877 + PP_0126 0.11 +0.5 131,995 + PP_0126 0.30 -1.1 132,019 - PP_0126 0.34 -0.1 132,036 + PP_0126 0.37 +0.9 132,037 - PP_0126 0.37 -2.4 132,103 + PP_0126 0.48 -2.0 132,286 + PP_0126 0.77 -1.8 132,406 + -2.0 132,406 + -1.0 132,407 - -2.8 132,424 + -0.1 132,443 + +1.0 132,443 + -1.3 132,444 - +1.6 132,444 - -1.7 132,444 - -0.0 132,444 - -2.7 132,502 - +0.7 132,502 - +2.3 132,502 - +1.1 132,502 - -1.0 132,572 + -0.2 132,572 + +0.8 132,572 + +1.2 132,573 - +0.1 132,583 + +2.8 132,584 - -0.2 132,584 - -0.3 132,584 - +0.1 132,671 + PP_0127 0.15 +1.2 132,671 + PP_0127 0.15 +1.5 132,672 - PP_0127 0.15 +0.4 132,672 - PP_0127 0.15 +2.6 132,672 - PP_0127 0.15 +1.8 132,740 + PP_0127 0.26 -1.1 133,080 - PP_0127 0.79 +0.3 133,080 - PP_0127 0.79 +1.7 133,096 - PP_0127 0.82 +1.1 133,122 - PP_0127 0.86 +1.1 133,208 + +0.5 133,208 + -2.1 133,208 + -0.3 133,240 + +0.2 133,240 + -0.9 133,240 + +0.8 133,240 + -0.9 133,240 + +1.3 133,241 - -1.1 133,387 + PP_0128 0.19 +1.6 133,387 + PP_0128 0.19 -0.2 133,388 - PP_0128 0.19 +1.0 133,511 + PP_0128 0.34 +0.8 133,512 - PP_0128 0.34 +0.3 133,562 + PP_0128 0.40 +0.8 133,562 + PP_0128 0.40 +0.1 133,563 - PP_0128 0.40 -2.2 133,720 - PP_0128 0.58 +0.5 133,775 + PP_0128 0.65 +0.5 133,775 + PP_0128 0.65 +0.5 133,775 + PP_0128 0.65 +0.8 133,776 - PP_0128 0.65 +1.2 133,777 + PP_0128 0.65 +1.2 133,778 - PP_0128 0.65 -0.2 133,800 - PP_0128 0.67 -1.1 133,942 + PP_0128 0.84 -0.6 133,942 + PP_0128 0.84 +0.3 133,943 - PP_0128 0.84 -0.3 133,943 - PP_0128 0.84 +1.0 133,943 - PP_0128 0.84 +0.3 133,943 - PP_0128 0.84 -1.9 134,056 + -0.4 134,056 + -1.7 134,056 + +0.8 134,075 + +0.4 134,076 - -0.6 134,076 - -1.3 134,139 - +0.1 134,143 + -0.7 134,143 + -2.3
Or see this region's nucleotide sequence