Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1977 and PP_t25 are separated by 280 nucleotides PP_t25 and PP_t26 are separated by 72 nucleotides PP_t26 and PP_t27 are separated by 51 nucleotides PP_t27 and PP_t28 are separated by 42 nucleotides PP_t28 and PP_t29 are separated by 61 nucleotides PP_t29 and PP_t30 are separated by 42 nucleotides PP_t30 and PP_1978 are separated by 170 nucleotides PP_1978 and PP_1979 are separated by 15 nucleotides
PP_1977: PP_1977 - Glutamate--tRNA ligase, at 2,239,772 to 2,241,253
_1977
PP_t25: PP_t25 - tRNA-Ala, at 2,241,534 to 2,241,609
_t25
PP_t26: PP_t26 - tRNA-Glu, at 2,241,682 to 2,241,757
_t26
PP_t27: PP_t27 - tRNA-Ala, at 2,241,809 to 2,241,884
_t27
PP_t28: PP_t28 - tRNA-Glu, at 2,241,927 to 2,242,002
_t28
PP_t29: PP_t29 - tRNA-Ala, at 2,242,064 to 2,242,139
_t29
PP_t30: PP_t30 - tRNA-Glu, at 2,242,182 to 2,242,257
_t30
PP_1978: PP_1978 - Transcriptional regulator, TetR family, at 2,242,428 to 2,242,967
_1978
PP_1979: PP_1979 - putative Hydrolase, at 2,242,983 to 2,243,864
_1979
Position (kb)
2242
2243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2241.268 kb on + strand at 2241.352 kb on + strand at 2241.391 kb on + strand at 2241.762 kb on + strand at 2241.778 kb on + strand at 2241.874 kb on + strand, within PP_t27 at 2242.026 kb on + strand at 2242.072 kb on - strand, within PP_t29 at 2242.072 kb on - strand at 2242.072 kb on - strand, within PP_t29 at 2242.072 kb on - strand, within PP_t29 at 2242.129 kb on + strand, within PP_t29 at 2242.180 kb on - strand at 2242.284 kb on - strand at 2242.379 kb on - strand at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.560 kb on + strand, within PP_1978 at 2242.561 kb on - strand, within PP_1978 at 2242.561 kb on - strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.589 kb on + strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.590 kb on - strand, within PP_1978 at 2242.695 kb on + strand, within PP_1978 at 2242.695 kb on + strand, within PP_1978 at 2242.696 kb on - strand, within PP_1978 at 2242.726 kb on + strand, within PP_1978 at 2242.726 kb on + strand, within PP_1978 at 2242.727 kb on - strand, within PP_1978 at 2242.988 kb on + strand at 2242.989 kb on - strand at 2242.989 kb on - strand at 2243.139 kb on - strand, within PP_1979 at 2243.139 kb on - strand, within PP_1979
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 2,241,268 + +0.6 2,241,352 + +0.0 2,241,391 + +1.0 2,241,762 + +2.8 2,241,778 + -1.0 2,241,874 + PP_t27 0.86 -0.2 2,242,026 + +0.3 2,242,072 - PP_t29 0.11 -2.0 2,242,072 - -1.6 2,242,072 - PP_t29 0.11 -1.7 2,242,072 - PP_t29 0.11 +0.1 2,242,129 + PP_t29 0.86 -0.1 2,242,180 - -2.1 2,242,284 - -1.6 2,242,379 - +0.7 2,242,560 + PP_1978 0.24 -0.2 2,242,560 + PP_1978 0.24 -1.8 2,242,560 + PP_1978 0.24 -0.3 2,242,560 + PP_1978 0.24 +0.5 2,242,561 - PP_1978 0.25 -1.8 2,242,561 - PP_1978 0.25 -2.8 2,242,589 + PP_1978 0.30 +1.0 2,242,589 + PP_1978 0.30 -2.0 2,242,589 + PP_1978 0.30 +0.1 2,242,589 + PP_1978 0.30 -1.0 2,242,590 - PP_1978 0.30 -0.1 2,242,590 - PP_1978 0.30 +1.5 2,242,590 - PP_1978 0.30 -0.1 2,242,590 - PP_1978 0.30 -0.5 2,242,695 + PP_1978 0.49 -2.2 2,242,695 + PP_1978 0.49 +0.7 2,242,696 - PP_1978 0.50 +0.3 2,242,726 + PP_1978 0.55 +3.2 2,242,726 + PP_1978 0.55 -0.1 2,242,727 - PP_1978 0.55 +0.4 2,242,988 + -1.1 2,242,989 - -0.5 2,242,989 - -0.1 2,243,139 - PP_1979 0.18 +0.6 2,243,139 - PP_1979 0.18 +0.8
Or see this region's nucleotide sequence