Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1501 and PP_1502 are separated by 141 nucleotides PP_1502 and PP_1503 are separated by 50 nucleotides PP_1503 and PP_1504 are separated by 219 nucleotides PP_1504 and PP_1505 are separated by 148 nucleotides
PP_1501: PP_1501 - conserved protein of unknown function, at 1,706,856 to 1,707,182
_1501
PP_1502: PP_1502 - OmpA family protein, at 1,707,324 to 1,708,115
_1502
PP_1503: PP_1503 - conserved protein of unknown function, at 1,708,166 to 1,708,636
_1503
PP_1504: PP_1504 - conserved protein of unknown function, at 1,708,856 to 1,709,233
_1504
PP_1505: PP_1505 - Phosphoenolpyruvate carboxylase, at 1,709,382 to 1,712,009
_1505
Position (kb)
1708
1709 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1707.547 kb on + strand, within PP_1502 at 1707.547 kb on + strand, within PP_1502 at 1707.548 kb on - strand, within PP_1502 at 1707.548 kb on - strand, within PP_1502 at 1707.574 kb on + strand, within PP_1502 at 1707.574 kb on + strand, within PP_1502 at 1707.574 kb on + strand, within PP_1502 at 1707.574 kb on + strand, within PP_1502 at 1707.656 kb on + strand, within PP_1502 at 1707.828 kb on - strand, within PP_1502 at 1707.828 kb on - strand, within PP_1502 at 1707.878 kb on - strand at 1707.920 kb on - strand, within PP_1502 at 1707.920 kb on - strand, within PP_1502 at 1708.102 kb on - strand at 1708.115 kb on + strand at 1708.115 kb on + strand at 1708.115 kb on + strand at 1708.116 kb on - strand at 1708.116 kb on - strand at 1708.116 kb on - strand at 1708.116 kb on - strand at 1708.196 kb on + strand at 1708.197 kb on - strand at 1708.443 kb on + strand, within PP_1503 at 1708.444 kb on - strand, within PP_1503 at 1708.444 kb on - strand, within PP_1503 at 1708.489 kb on + strand, within PP_1503 at 1708.490 kb on - strand, within PP_1503 at 1708.550 kb on - strand, within PP_1503 at 1708.610 kb on - strand at 1708.636 kb on + strand at 1708.726 kb on + strand at 1708.727 kb on - strand at 1708.758 kb on + strand at 1708.758 kb on + strand at 1708.759 kb on - strand at 1708.759 kb on - strand at 1708.931 kb on - strand, within PP_1504 at 1708.948 kb on - strand, within PP_1504 at 1708.994 kb on + strand, within PP_1504 at 1708.994 kb on + strand, within PP_1504 at 1708.994 kb on + strand, within PP_1504 at 1708.994 kb on + strand, within PP_1504 at 1708.994 kb on + strand, within PP_1504 at 1708.994 kb on + strand, within PP_1504 at 1708.994 kb on + strand, within PP_1504 at 1708.995 kb on - strand at 1708.995 kb on - strand, within PP_1504 at 1708.995 kb on - strand, within PP_1504 at 1708.995 kb on - strand, within PP_1504 at 1708.998 kb on + strand, within PP_1504 at 1708.998 kb on + strand, within PP_1504 at 1708.998 kb on + strand, within PP_1504 at 1708.998 kb on + strand, within PP_1504 at 1708.998 kb on + strand, within PP_1504 at 1708.998 kb on + strand, within PP_1504 at 1708.998 kb on + strand, within PP_1504 at 1709.076 kb on + strand, within PP_1504 at 1709.077 kb on - strand, within PP_1504 at 1709.358 kb on - strand at 1709.358 kb on - strand at 1709.358 kb on - strand at 1709.411 kb on - strand at 1709.634 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 1,707,547 + PP_1502 0.28 -2.1 1,707,547 + PP_1502 0.28 +0.3 1,707,548 - PP_1502 0.28 -3.0 1,707,548 - PP_1502 0.28 -0.4 1,707,574 + PP_1502 0.32 -2.5 1,707,574 + PP_1502 0.32 -2.4 1,707,574 + PP_1502 0.32 -1.1 1,707,574 + PP_1502 0.32 -0.4 1,707,656 + PP_1502 0.42 +1.5 1,707,828 - PP_1502 0.64 +1.6 1,707,828 - PP_1502 0.64 -0.5 1,707,878 - +1.4 1,707,920 - PP_1502 0.75 -0.9 1,707,920 - PP_1502 0.75 -0.0 1,708,102 - -0.1 1,708,115 + -0.5 1,708,115 + +0.5 1,708,115 + +0.2 1,708,116 - -2.1 1,708,116 - -0.6 1,708,116 - +0.4 1,708,116 - -0.8 1,708,196 + +0.1 1,708,197 - +1.3 1,708,443 + PP_1503 0.59 +0.1 1,708,444 - PP_1503 0.59 +1.1 1,708,444 - PP_1503 0.59 +1.4 1,708,489 + PP_1503 0.69 -2.9 1,708,490 - PP_1503 0.69 -1.9 1,708,550 - PP_1503 0.82 +1.7 1,708,610 - -0.9 1,708,636 + +0.9 1,708,726 + +0.5 1,708,727 - +0.3 1,708,758 + +0.1 1,708,758 + +0.5 1,708,759 - -0.9 1,708,759 - +0.6 1,708,931 - PP_1504 0.20 -2.6 1,708,948 - PP_1504 0.24 +0.4 1,708,994 + PP_1504 0.37 -2.1 1,708,994 + PP_1504 0.37 -0.2 1,708,994 + PP_1504 0.37 -0.3 1,708,994 + PP_1504 0.37 +1.1 1,708,994 + PP_1504 0.37 +1.7 1,708,994 + PP_1504 0.37 +1.5 1,708,994 + PP_1504 0.37 -1.3 1,708,995 - -1.4 1,708,995 - PP_1504 0.37 +0.3 1,708,995 - PP_1504 0.37 -0.4 1,708,995 - PP_1504 0.37 -1.9 1,708,998 + PP_1504 0.38 -0.1 1,708,998 + PP_1504 0.38 -0.1 1,708,998 + PP_1504 0.38 -1.7 1,708,998 + PP_1504 0.38 -0.7 1,708,998 + PP_1504 0.38 -0.3 1,708,998 + PP_1504 0.38 +0.4 1,708,998 + PP_1504 0.38 -0.5 1,709,076 + PP_1504 0.58 -0.2 1,709,077 - PP_1504 0.58 +0.1 1,709,358 - -2.0 1,709,358 - -0.1 1,709,358 - +1.6 1,709,411 - -0.5 1,709,634 + -1.7
Or see this region's nucleotide sequence