Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0432 and PP_0433 are separated by 99 nucleotides PP_0433 and PP_0434 are separated by 59 nucleotides PP_0434 and PP_0435 are separated by 3 nucleotides
PP_0432: PP_0432 - N-acetyl-gamma-glutamyl-phosphate reductase 1, at 518,612 to 519,646
_0432
PP_0433: PP_0433 - Iron-sulfur cluster insertion protein ErpA, at 519,746 to 520,144
_0433
PP_0434: PP_0434 - Anhydro-N-acetylmuramic acid kinase, at 520,204 to 521,295
_0434
PP_0435: PP_0435 - Peptidase, M23/M37 family, at 521,299 to 522,726
_0435
Position (kb) 520
521
522 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 519.367 kb on + strand, within PP_0432 at 519.764 kb on + strand at 520.142 kb on + strand at 520.143 kb on - strand at 520.155 kb on + strand at 520.155 kb on + strand at 520.205 kb on + strand at 520.337 kb on - strand, within PP_0434 at 520.343 kb on + strand, within PP_0434 at 520.375 kb on + strand, within PP_0434 at 520.375 kb on + strand, within PP_0434 at 520.376 kb on - strand, within PP_0434 at 520.692 kb on - strand, within PP_0434 at 520.692 kb on - strand, within PP_0434 at 520.692 kb on - strand, within PP_0434 at 520.807 kb on + strand, within PP_0434 at 520.807 kb on + strand, within PP_0434 at 520.808 kb on - strand, within PP_0434 at 520.813 kb on - strand, within PP_0434 at 520.978 kb on - strand, within PP_0434 at 521.076 kb on + strand, within PP_0434 at 521.077 kb on - strand, within PP_0434 at 521.195 kb on + strand at 521.196 kb on - strand at 521.285 kb on + strand at 521.285 kb on + strand at 521.285 kb on + strand at 521.286 kb on - strand at 521.301 kb on - strand at 521.466 kb on - strand, within PP_0435 at 521.507 kb on + strand, within PP_0435 at 521.507 kb on + strand, within PP_0435 at 521.508 kb on - strand, within PP_0435 at 521.580 kb on - strand, within PP_0435 at 521.591 kb on + strand, within PP_0435 at 521.592 kb on - strand, within PP_0435 at 521.837 kb on + strand, within PP_0435 at 521.837 kb on + strand, within PP_0435 at 521.837 kb on + strand, within PP_0435 at 521.838 kb on - strand, within PP_0435 at 521.838 kb on - strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.840 kb on + strand, within PP_0435 at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand, within PP_0435 at 521.841 kb on - strand at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.844 kb on + strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.845 kb on - strand, within PP_0435 at 521.882 kb on + strand, within PP_0435 at 521.883 kb on - strand, within PP_0435 at 521.883 kb on - strand, within PP_0435 at 521.963 kb on + strand, within PP_0435 at 521.964 kb on - strand, within PP_0435 at 522.017 kb on + strand, within PP_0435 at 522.113 kb on + strand, within PP_0435 at 522.113 kb on + strand, within PP_0435 at 522.125 kb on - strand, within PP_0435 at 522.159 kb on - strand, within PP_0435
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 519,367 + PP_0432 0.73 -0.1 519,764 + +0.5 520,142 + -2.2 520,143 - +0.3 520,155 + +0.2 520,155 + -0.6 520,205 + -1.1 520,337 - PP_0434 0.12 +2.0 520,343 + PP_0434 0.13 +2.6 520,375 + PP_0434 0.16 +2.0 520,375 + PP_0434 0.16 +1.3 520,376 - PP_0434 0.16 -0.8 520,692 - PP_0434 0.45 +1.0 520,692 - PP_0434 0.45 -0.3 520,692 - PP_0434 0.45 +1.2 520,807 + PP_0434 0.55 +0.6 520,807 + PP_0434 0.55 -0.6 520,808 - PP_0434 0.55 -2.2 520,813 - PP_0434 0.56 -2.3 520,978 - PP_0434 0.71 +2.1 521,076 + PP_0434 0.80 -1.1 521,077 - PP_0434 0.80 -1.1 521,195 + -2.6 521,196 - +0.3 521,285 + -0.5 521,285 + +2.0 521,285 + -1.0 521,286 - -0.0 521,301 - +0.4 521,466 - PP_0435 0.12 -2.2 521,507 + PP_0435 0.15 +2.0 521,507 + PP_0435 0.15 -0.5 521,508 - PP_0435 0.15 -1.0 521,580 - PP_0435 0.20 +0.9 521,591 + PP_0435 0.20 -1.2 521,592 - PP_0435 0.21 -1.1 521,837 + PP_0435 0.38 -2.3 521,837 + PP_0435 0.38 +0.3 521,837 + PP_0435 0.38 -0.3 521,838 - PP_0435 0.38 -1.9 521,838 - PP_0435 0.38 -2.4 521,840 + PP_0435 0.38 +0.4 521,840 + PP_0435 0.38 +3.0 521,840 + PP_0435 0.38 -2.7 521,840 + PP_0435 0.38 -0.5 521,840 + PP_0435 0.38 +1.0 521,841 - PP_0435 0.38 +0.5 521,841 - PP_0435 0.38 -3.0 521,841 - +2.0 521,841 - PP_0435 0.38 -2.1 521,841 - PP_0435 0.38 -0.6 521,841 - PP_0435 0.38 +1.0 521,841 - +3.0 521,844 + PP_0435 0.38 -0.7 521,844 + PP_0435 0.38 +0.3 521,844 + PP_0435 0.38 +1.4 521,844 + PP_0435 0.38 -0.6 521,844 + PP_0435 0.38 +2.5 521,844 + PP_0435 0.38 -1.9 521,845 - PP_0435 0.38 +0.7 521,845 - PP_0435 0.38 +2.5 521,845 - PP_0435 0.38 -1.3 521,845 - PP_0435 0.38 +1.4 521,845 - PP_0435 0.38 +2.4 521,882 + PP_0435 0.41 +2.0 521,883 - PP_0435 0.41 -0.2 521,883 - PP_0435 0.41 +0.9 521,963 + PP_0435 0.46 -2.2 521,964 - PP_0435 0.47 -2.1 522,017 + PP_0435 0.50 -0.8 522,113 + PP_0435 0.57 -1.8 522,113 + PP_0435 0.57 -0.9 522,125 - PP_0435 0.58 -1.1 522,159 - PP_0435 0.60 -2.5
Or see this region's nucleotide sequence