Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0399 and PP_0400 overlap by 1 nucleotides PP_0400 and PP_0401 are separated by 197 nucleotides PP_0401 and PP_0402 overlap by 4 nucleotides PP_0402 and PP_0403 overlap by 4 nucleotides
PP_0399: PP_0399 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical, at 485,068 to 485,934
_0399
PP_0400: PP_0400 - CO2+/MG2+ efflux protein, at 485,934 to 486,314
_0400
PP_0401: PP_0401 - Ribosomal RNA small subunit methyltransferase A, at 486,512 to 487,315
_0401
PP_0402: PP_0402 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 487,312 to 488,301
_0402
PP_0403: PP_0403 - peptidyl-prolyl cis-trans isomerase, at 488,298 to 489,617
_0403
Position (kb) 486
487
488 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 486.017 kb on - strand, within PP_0400 at 486.067 kb on + strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.126 kb on + strand, within PP_0400 at 486.126 kb on + strand, within PP_0400 at 486.127 kb on - strand, within PP_0400 at 486.136 kb on - strand, within PP_0400 at 486.136 kb on - strand, within PP_0400 at 486.220 kb on + strand, within PP_0400 at 486.221 kb on - strand, within PP_0400 at 486.343 kb on - strand at 486.343 kb on - strand at 486.343 kb on - strand at 486.353 kb on - strand at 486.428 kb on + strand at 486.440 kb on + strand at 486.508 kb on + strand at 486.829 kb on - strand, within PP_0401 at 486.829 kb on - strand, within PP_0401 at 486.978 kb on + strand, within PP_0401 at 486.978 kb on + strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 487.302 kb on + strand at 487.303 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 486,017 - PP_0400 0.22 -0.1 486,067 + PP_0400 0.35 +0.7 486,068 - PP_0400 0.35 -0.5 486,068 - PP_0400 0.35 +0.1 486,068 - PP_0400 0.35 -1.0 486,068 - PP_0400 0.35 -2.9 486,126 + PP_0400 0.50 -2.5 486,126 + PP_0400 0.50 +2.3 486,127 - PP_0400 0.51 -0.6 486,136 - PP_0400 0.53 -1.4 486,136 - PP_0400 0.53 +0.5 486,220 + PP_0400 0.75 +0.2 486,221 - PP_0400 0.75 -1.5 486,343 - -0.6 486,343 - -0.4 486,343 - +0.3 486,353 - -1.6 486,428 + +0.8 486,440 + -1.1 486,508 + -1.0 486,829 - PP_0401 0.39 -2.1 486,829 - PP_0401 0.39 +0.2 486,978 + PP_0401 0.58 +0.4 486,978 + PP_0401 0.58 -1.6 486,979 - PP_0401 0.58 -2.2 486,979 - PP_0401 0.58 -2.2 486,979 - PP_0401 0.58 -0.2 487,302 + +0.9 487,303 - +0.5
Or see this region's nucleotide sequence